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TupA: A Tungstate Binding Protein in the Periplasm of Desulfovibrio alaskensis G20

The TupABC system is involved in the cellular uptake of tungsten and belongs to the ABC (ATP binding cassette)-type transporter systems. The TupA component is a periplasmic protein that binds tungstate anions, which are then transported through the membrane by the TupB component using ATP hydrolysis...

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Autores principales: Otrelo-Cardoso, Ana Rita, Nair, Rashmi R., Correia, Márcia A. S., Rivas, Maria G., Santos-Silva, Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139814/
https://www.ncbi.nlm.nih.gov/pubmed/24992597
http://dx.doi.org/10.3390/ijms150711783
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author Otrelo-Cardoso, Ana Rita
Nair, Rashmi R.
Correia, Márcia A. S.
Rivas, Maria G.
Santos-Silva, Teresa
author_facet Otrelo-Cardoso, Ana Rita
Nair, Rashmi R.
Correia, Márcia A. S.
Rivas, Maria G.
Santos-Silva, Teresa
author_sort Otrelo-Cardoso, Ana Rita
collection PubMed
description The TupABC system is involved in the cellular uptake of tungsten and belongs to the ABC (ATP binding cassette)-type transporter systems. The TupA component is a periplasmic protein that binds tungstate anions, which are then transported through the membrane by the TupB component using ATP hydrolysis as the energy source (the reaction catalyzed by the ModC component). We report the heterologous expression, purification, determination of affinity binding constants and crystallization of the Desulfovibrio alaskensis G20 TupA. The tupA gene (locus tag Dde_0234) was cloned in the pET46 Enterokinase/Ligation-Independent Cloning (LIC) expression vector, and the construct was used to transform BL21 (DE3) cells. TupA expression and purification were optimized to a final yield of 10 mg of soluble pure protein per liter of culture medium. Native polyacrylamide gel electrophoresis was carried out showing that TupA binds both tungstate and molybdate ions and has no significant interaction with sulfate, phosphate or perchlorate. Quantitative analysis of metal binding by isothermal titration calorimetry was in agreement with these results, but in addition, shows that TupA has higher affinity to tungstate than molybdate. The protein crystallizes in the presence of 30% (w/v) polyethylene glycol 3350 using the hanging-drop vapor diffusion method. The crystals diffract X-rays beyond 1.4 Å resolution and belong to the P2(1) space group, with cell parameters a = 52.25 Å, b = 42.50 Å, c = 54.71 Å, β = 95.43°. A molecular replacement solution was found, and the structure is currently under refinement.
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spelling pubmed-41398142014-08-21 TupA: A Tungstate Binding Protein in the Periplasm of Desulfovibrio alaskensis G20 Otrelo-Cardoso, Ana Rita Nair, Rashmi R. Correia, Márcia A. S. Rivas, Maria G. Santos-Silva, Teresa Int J Mol Sci Article The TupABC system is involved in the cellular uptake of tungsten and belongs to the ABC (ATP binding cassette)-type transporter systems. The TupA component is a periplasmic protein that binds tungstate anions, which are then transported through the membrane by the TupB component using ATP hydrolysis as the energy source (the reaction catalyzed by the ModC component). We report the heterologous expression, purification, determination of affinity binding constants and crystallization of the Desulfovibrio alaskensis G20 TupA. The tupA gene (locus tag Dde_0234) was cloned in the pET46 Enterokinase/Ligation-Independent Cloning (LIC) expression vector, and the construct was used to transform BL21 (DE3) cells. TupA expression and purification were optimized to a final yield of 10 mg of soluble pure protein per liter of culture medium. Native polyacrylamide gel electrophoresis was carried out showing that TupA binds both tungstate and molybdate ions and has no significant interaction with sulfate, phosphate or perchlorate. Quantitative analysis of metal binding by isothermal titration calorimetry was in agreement with these results, but in addition, shows that TupA has higher affinity to tungstate than molybdate. The protein crystallizes in the presence of 30% (w/v) polyethylene glycol 3350 using the hanging-drop vapor diffusion method. The crystals diffract X-rays beyond 1.4 Å resolution and belong to the P2(1) space group, with cell parameters a = 52.25 Å, b = 42.50 Å, c = 54.71 Å, β = 95.43°. A molecular replacement solution was found, and the structure is currently under refinement. MDPI 2014-07-02 /pmc/articles/PMC4139814/ /pubmed/24992597 http://dx.doi.org/10.3390/ijms150711783 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Otrelo-Cardoso, Ana Rita
Nair, Rashmi R.
Correia, Márcia A. S.
Rivas, Maria G.
Santos-Silva, Teresa
TupA: A Tungstate Binding Protein in the Periplasm of Desulfovibrio alaskensis G20
title TupA: A Tungstate Binding Protein in the Periplasm of Desulfovibrio alaskensis G20
title_full TupA: A Tungstate Binding Protein in the Periplasm of Desulfovibrio alaskensis G20
title_fullStr TupA: A Tungstate Binding Protein in the Periplasm of Desulfovibrio alaskensis G20
title_full_unstemmed TupA: A Tungstate Binding Protein in the Periplasm of Desulfovibrio alaskensis G20
title_short TupA: A Tungstate Binding Protein in the Periplasm of Desulfovibrio alaskensis G20
title_sort tupa: a tungstate binding protein in the periplasm of desulfovibrio alaskensis g20
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139814/
https://www.ncbi.nlm.nih.gov/pubmed/24992597
http://dx.doi.org/10.3390/ijms150711783
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