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Value of Micro-CT for Monitoring Spinal Microvascular Changes after Chronic Spinal Cord Compression

Neurological degeneration can occur after compression of the spinal cord. It is widely accepted that spinal cord compression leads to ischemic lesions and ultimately neurological dysfunction due to a narrowed spinal canal. Therefore, an in-depth understanding of the pathogenesis of spinal cord compr...

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Autores principales: Long, Hou-Qing, Xie, Wen-Han, Chen, Wen-Li, Xie, Wen-Lin, Xu, Jing-Hui, Hu, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139829/
https://www.ncbi.nlm.nih.gov/pubmed/25003643
http://dx.doi.org/10.3390/ijms150712061
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author Long, Hou-Qing
Xie, Wen-Han
Chen, Wen-Li
Xie, Wen-Lin
Xu, Jing-Hui
Hu, Yong
author_facet Long, Hou-Qing
Xie, Wen-Han
Chen, Wen-Li
Xie, Wen-Lin
Xu, Jing-Hui
Hu, Yong
author_sort Long, Hou-Qing
collection PubMed
description Neurological degeneration can occur after compression of the spinal cord. It is widely accepted that spinal cord compression leads to ischemic lesions and ultimately neurological dysfunction due to a narrowed spinal canal. Therefore, an in-depth understanding of the pathogenesis of spinal cord compression injury is required to help develop effective clinical interventions. In the present study, we propose a new method of quantitative 3D micro-CT to observe microvascular events in a chronic spinal cord compression rat model. A total of 36 rats were divided into two groups: sham control group (n = 12) and compressive spinal cord injury group (n = 24). Rats were scarified at four weeks after surgery. In each group, CD34 micro-vessel immunohistochemical staining was performed in half of the animals, while micro-CT scanning was performed in the other half. Microvessel density (MVD) was measured after immunohistochemical staining, while the vascular index (VI) was measured in 3D micro-CT. In comparison with sham control, abnormal somatosensory evoked potentials (SEP) can be seen in all 24 cases of the compression group, and VI shows the amount of microvessels reduced consistently and significantly (p < 0.01). A significant correlation is also found between MVD and VI (r = 0.95, p < 0.01). These data suggest that quantitative 3D micro-CT is a sensitive and promising tool for investigating microvascular changes during chronic compressive spinal cord injury.
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spelling pubmed-41398292014-08-21 Value of Micro-CT for Monitoring Spinal Microvascular Changes after Chronic Spinal Cord Compression Long, Hou-Qing Xie, Wen-Han Chen, Wen-Li Xie, Wen-Lin Xu, Jing-Hui Hu, Yong Int J Mol Sci Article Neurological degeneration can occur after compression of the spinal cord. It is widely accepted that spinal cord compression leads to ischemic lesions and ultimately neurological dysfunction due to a narrowed spinal canal. Therefore, an in-depth understanding of the pathogenesis of spinal cord compression injury is required to help develop effective clinical interventions. In the present study, we propose a new method of quantitative 3D micro-CT to observe microvascular events in a chronic spinal cord compression rat model. A total of 36 rats were divided into two groups: sham control group (n = 12) and compressive spinal cord injury group (n = 24). Rats were scarified at four weeks after surgery. In each group, CD34 micro-vessel immunohistochemical staining was performed in half of the animals, while micro-CT scanning was performed in the other half. Microvessel density (MVD) was measured after immunohistochemical staining, while the vascular index (VI) was measured in 3D micro-CT. In comparison with sham control, abnormal somatosensory evoked potentials (SEP) can be seen in all 24 cases of the compression group, and VI shows the amount of microvessels reduced consistently and significantly (p < 0.01). A significant correlation is also found between MVD and VI (r = 0.95, p < 0.01). These data suggest that quantitative 3D micro-CT is a sensitive and promising tool for investigating microvascular changes during chronic compressive spinal cord injury. MDPI 2014-07-07 /pmc/articles/PMC4139829/ /pubmed/25003643 http://dx.doi.org/10.3390/ijms150712061 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Long, Hou-Qing
Xie, Wen-Han
Chen, Wen-Li
Xie, Wen-Lin
Xu, Jing-Hui
Hu, Yong
Value of Micro-CT for Monitoring Spinal Microvascular Changes after Chronic Spinal Cord Compression
title Value of Micro-CT for Monitoring Spinal Microvascular Changes after Chronic Spinal Cord Compression
title_full Value of Micro-CT for Monitoring Spinal Microvascular Changes after Chronic Spinal Cord Compression
title_fullStr Value of Micro-CT for Monitoring Spinal Microvascular Changes after Chronic Spinal Cord Compression
title_full_unstemmed Value of Micro-CT for Monitoring Spinal Microvascular Changes after Chronic Spinal Cord Compression
title_short Value of Micro-CT for Monitoring Spinal Microvascular Changes after Chronic Spinal Cord Compression
title_sort value of micro-ct for monitoring spinal microvascular changes after chronic spinal cord compression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139829/
https://www.ncbi.nlm.nih.gov/pubmed/25003643
http://dx.doi.org/10.3390/ijms150712061
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