Cargando…
Role of auxin during intercellular infection of Discaria trinervis by Frankia
Nitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the mo...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139986/ https://www.ncbi.nlm.nih.gov/pubmed/25191330 http://dx.doi.org/10.3389/fpls.2014.00399 |
_version_ | 1782331446657024000 |
---|---|
author | Imanishi, Leandro Perrine-Walker, Francine M. Ndour, Adama Vayssières, Alice Conejero, Genevieve Lucas, Mikaël Champion, Antony Laplaze, Laurent Wall, Luis Svistoonoff, Sergio |
author_facet | Imanishi, Leandro Perrine-Walker, Francine M. Ndour, Adama Vayssières, Alice Conejero, Genevieve Lucas, Mikaël Champion, Antony Laplaze, Laurent Wall, Luis Svistoonoff, Sergio |
author_sort | Imanishi, Leandro |
collection | PubMed |
description | Nitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected actinorhizal plant Casuarina glauca. Immunolocalisation experiments showed that auxin accumulated in Frankia-infected cells in both systems. We then characterized the expression of auxin transporters in D. trinervis nodules. No activation of the heterologous CgAUX1 promoter was detected in infected cells in D. trinervis. These results were confirmed with the endogenous D. trinervis gene, DtAUX1. However, DtAUX1 was expressed in the nodule meristem. Consistently, transgenic D. trinervis plants containing the auxin response marker DR5:VENUS showed expression of the reporter gene in the meristem. Immunolocalisation experiments using an antibody against the auxin efflux carrier PIN1, revealed the presence of this transporter in the plasma membrane of infected cells. Finally, we used in silico cellular models to analyse auxin fluxes in D. trinervis nodules. Our results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses. |
format | Online Article Text |
id | pubmed-4139986 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-41399862014-09-04 Role of auxin during intercellular infection of Discaria trinervis by Frankia Imanishi, Leandro Perrine-Walker, Francine M. Ndour, Adama Vayssières, Alice Conejero, Genevieve Lucas, Mikaël Champion, Antony Laplaze, Laurent Wall, Luis Svistoonoff, Sergio Front Plant Sci Plant Science Nitrogen-fixing nodules induced by Frankia in the actinorhizal plant Discaria trinervis result from a primitive intercellular root invasion pathway that does not involve root hair deformation and infection threads. Here, we analyzed the role of auxin in this intercellular infection pathway at the molecular level and compared it with our previous work in the intracellular infected actinorhizal plant Casuarina glauca. Immunolocalisation experiments showed that auxin accumulated in Frankia-infected cells in both systems. We then characterized the expression of auxin transporters in D. trinervis nodules. No activation of the heterologous CgAUX1 promoter was detected in infected cells in D. trinervis. These results were confirmed with the endogenous D. trinervis gene, DtAUX1. However, DtAUX1 was expressed in the nodule meristem. Consistently, transgenic D. trinervis plants containing the auxin response marker DR5:VENUS showed expression of the reporter gene in the meristem. Immunolocalisation experiments using an antibody against the auxin efflux carrier PIN1, revealed the presence of this transporter in the plasma membrane of infected cells. Finally, we used in silico cellular models to analyse auxin fluxes in D. trinervis nodules. Our results point to the existence of divergent roles of auxin in intercellularly- and intracellularly-infected actinorhizal plants, an ancestral infection pathways leading to root nodule symbioses. Frontiers Media S.A. 2014-08-21 /pmc/articles/PMC4139986/ /pubmed/25191330 http://dx.doi.org/10.3389/fpls.2014.00399 Text en Copyright © 2014 Imanishi, Perrine-Walker, Ndour, Vayssières, Conejero, Lucas, Champion, Laplaze, Wall and Svistoonoff. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Imanishi, Leandro Perrine-Walker, Francine M. Ndour, Adama Vayssières, Alice Conejero, Genevieve Lucas, Mikaël Champion, Antony Laplaze, Laurent Wall, Luis Svistoonoff, Sergio Role of auxin during intercellular infection of Discaria trinervis by Frankia |
title | Role of auxin during intercellular infection of Discaria trinervis by Frankia |
title_full | Role of auxin during intercellular infection of Discaria trinervis by Frankia |
title_fullStr | Role of auxin during intercellular infection of Discaria trinervis by Frankia |
title_full_unstemmed | Role of auxin during intercellular infection of Discaria trinervis by Frankia |
title_short | Role of auxin during intercellular infection of Discaria trinervis by Frankia |
title_sort | role of auxin during intercellular infection of discaria trinervis by frankia |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4139986/ https://www.ncbi.nlm.nih.gov/pubmed/25191330 http://dx.doi.org/10.3389/fpls.2014.00399 |
work_keys_str_mv | AT imanishileandro roleofauxinduringintercellularinfectionofdiscariatrinervisbyfrankia AT perrinewalkerfrancinem roleofauxinduringintercellularinfectionofdiscariatrinervisbyfrankia AT ndouradama roleofauxinduringintercellularinfectionofdiscariatrinervisbyfrankia AT vayssieresalice roleofauxinduringintercellularinfectionofdiscariatrinervisbyfrankia AT conejerogenevieve roleofauxinduringintercellularinfectionofdiscariatrinervisbyfrankia AT lucasmikael roleofauxinduringintercellularinfectionofdiscariatrinervisbyfrankia AT championantony roleofauxinduringintercellularinfectionofdiscariatrinervisbyfrankia AT laplazelaurent roleofauxinduringintercellularinfectionofdiscariatrinervisbyfrankia AT wallluis roleofauxinduringintercellularinfectionofdiscariatrinervisbyfrankia AT svistoonoffsergio roleofauxinduringintercellularinfectionofdiscariatrinervisbyfrankia |