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Rapid Diagnosis of Childhood Pulmonary Tuberculosis by Xpert MTB/RIF Assay Using Bronchoalveolar Lavage Fluid

In order to evaluate the diagnostic accuracy of the Xpert MTB/RIF assay on childhood pulmonary tuberculosis (PTB) using bronchoalveolar lavage fluid (BALF), we evaluated the sensitivity, specificity, positive predictive value, and negative predictive value of Xpert MTB/RIF assay using BALF in compar...

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Autores principales: Yin, Qing-Qin, Jiao, Wei-Wei, Han, Rui, Jiao, An-Xia, Sun, Lin, Tian, Jian-Ling, Ma, Yu-Yan, Rao, Xiao-Chun, Shen, Chen, Li, Qin-Jing, Shen, A-Dong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140106/
https://www.ncbi.nlm.nih.gov/pubmed/25165698
http://dx.doi.org/10.1155/2014/310194
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author Yin, Qing-Qin
Jiao, Wei-Wei
Han, Rui
Jiao, An-Xia
Sun, Lin
Tian, Jian-Ling
Ma, Yu-Yan
Rao, Xiao-Chun
Shen, Chen
Li, Qin-Jing
Shen, A-Dong
author_facet Yin, Qing-Qin
Jiao, Wei-Wei
Han, Rui
Jiao, An-Xia
Sun, Lin
Tian, Jian-Ling
Ma, Yu-Yan
Rao, Xiao-Chun
Shen, Chen
Li, Qin-Jing
Shen, A-Dong
author_sort Yin, Qing-Qin
collection PubMed
description In order to evaluate the diagnostic accuracy of the Xpert MTB/RIF assay on childhood pulmonary tuberculosis (PTB) using bronchoalveolar lavage fluid (BALF), we evaluated the sensitivity, specificity, positive predictive value, and negative predictive value of Xpert MTB/RIF assay using BALF in comparison with acid-fast bacilli (AFB) microscopy and Mycobacterium tuberculosis (MTB) culture for diagnosing childhood PTB using Chinese “composite clinical reference standard” (CCRS) as reference standard. Two hundred fifty-five children with suspected PTB were enrolled at Beijing Children's Hospital from September 2010 to July 2013. Compared with Chinese CCRS, the sensitivity of AFB microscopy, MTB culture, and Xpert MTB/RIF assay was 8.4%, 28.9%, and 53.0%, respectively. The specificity of three assays was all 100%. Xpert MTB/RIF assay could detect 33.9% of cases with negative MTB culture, and 48.7% of cases with negative AFB microscopy. Younger age (<3 years), absence of BCG scar, and contact with TB patient were found significantly associated with a positive result of Xpert MTB/RIF assay. In conclusion, Xpert MTB/RIF assay using BALF can assist in diagnosing childhood PTB much faster when fiberoptic bronchoscopy is necessary according to the chest radiograph.
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spelling pubmed-41401062014-08-27 Rapid Diagnosis of Childhood Pulmonary Tuberculosis by Xpert MTB/RIF Assay Using Bronchoalveolar Lavage Fluid Yin, Qing-Qin Jiao, Wei-Wei Han, Rui Jiao, An-Xia Sun, Lin Tian, Jian-Ling Ma, Yu-Yan Rao, Xiao-Chun Shen, Chen Li, Qin-Jing Shen, A-Dong Biomed Res Int Research Article In order to evaluate the diagnostic accuracy of the Xpert MTB/RIF assay on childhood pulmonary tuberculosis (PTB) using bronchoalveolar lavage fluid (BALF), we evaluated the sensitivity, specificity, positive predictive value, and negative predictive value of Xpert MTB/RIF assay using BALF in comparison with acid-fast bacilli (AFB) microscopy and Mycobacterium tuberculosis (MTB) culture for diagnosing childhood PTB using Chinese “composite clinical reference standard” (CCRS) as reference standard. Two hundred fifty-five children with suspected PTB were enrolled at Beijing Children's Hospital from September 2010 to July 2013. Compared with Chinese CCRS, the sensitivity of AFB microscopy, MTB culture, and Xpert MTB/RIF assay was 8.4%, 28.9%, and 53.0%, respectively. The specificity of three assays was all 100%. Xpert MTB/RIF assay could detect 33.9% of cases with negative MTB culture, and 48.7% of cases with negative AFB microscopy. Younger age (<3 years), absence of BCG scar, and contact with TB patient were found significantly associated with a positive result of Xpert MTB/RIF assay. In conclusion, Xpert MTB/RIF assay using BALF can assist in diagnosing childhood PTB much faster when fiberoptic bronchoscopy is necessary according to the chest radiograph. Hindawi Publishing Corporation 2014 2014-08-06 /pmc/articles/PMC4140106/ /pubmed/25165698 http://dx.doi.org/10.1155/2014/310194 Text en Copyright © 2014 Qing-Qin Yin et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yin, Qing-Qin
Jiao, Wei-Wei
Han, Rui
Jiao, An-Xia
Sun, Lin
Tian, Jian-Ling
Ma, Yu-Yan
Rao, Xiao-Chun
Shen, Chen
Li, Qin-Jing
Shen, A-Dong
Rapid Diagnosis of Childhood Pulmonary Tuberculosis by Xpert MTB/RIF Assay Using Bronchoalveolar Lavage Fluid
title Rapid Diagnosis of Childhood Pulmonary Tuberculosis by Xpert MTB/RIF Assay Using Bronchoalveolar Lavage Fluid
title_full Rapid Diagnosis of Childhood Pulmonary Tuberculosis by Xpert MTB/RIF Assay Using Bronchoalveolar Lavage Fluid
title_fullStr Rapid Diagnosis of Childhood Pulmonary Tuberculosis by Xpert MTB/RIF Assay Using Bronchoalveolar Lavage Fluid
title_full_unstemmed Rapid Diagnosis of Childhood Pulmonary Tuberculosis by Xpert MTB/RIF Assay Using Bronchoalveolar Lavage Fluid
title_short Rapid Diagnosis of Childhood Pulmonary Tuberculosis by Xpert MTB/RIF Assay Using Bronchoalveolar Lavage Fluid
title_sort rapid diagnosis of childhood pulmonary tuberculosis by xpert mtb/rif assay using bronchoalveolar lavage fluid
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140106/
https://www.ncbi.nlm.nih.gov/pubmed/25165698
http://dx.doi.org/10.1155/2014/310194
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