Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA

[Image: see text] Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine...

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Detalles Bibliográficos
Autores principales: Fu, Lijuan, Guerrero, Candace R., Zhong, Na, Amato, Nicholas J., Liu, Yunhua, Liu, Shuo, Cai, Qian, Ji, Debin, Jin, Seung-Gi, Niedernhofer, Laura J., Pfeifer, Gerd P., Xu, Guo-Liang, Wang, Yinsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140497/
https://www.ncbi.nlm.nih.gov/pubmed/25073028
http://dx.doi.org/10.1021/ja505305z
Descripción
Sumario:[Image: see text] Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.

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