Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA

[Image: see text] Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine...

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Autores principales: Fu, Lijuan, Guerrero, Candace R., Zhong, Na, Amato, Nicholas J., Liu, Yunhua, Liu, Shuo, Cai, Qian, Ji, Debin, Jin, Seung-Gi, Niedernhofer, Laura J., Pfeifer, Gerd P., Xu, Guo-Liang, Wang, Yinsheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140497/
https://www.ncbi.nlm.nih.gov/pubmed/25073028
http://dx.doi.org/10.1021/ja505305z
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author Fu, Lijuan
Guerrero, Candace R.
Zhong, Na
Amato, Nicholas J.
Liu, Yunhua
Liu, Shuo
Cai, Qian
Ji, Debin
Jin, Seung-Gi
Niedernhofer, Laura J.
Pfeifer, Gerd P.
Xu, Guo-Liang
Wang, Yinsheng
author_facet Fu, Lijuan
Guerrero, Candace R.
Zhong, Na
Amato, Nicholas J.
Liu, Yunhua
Liu, Shuo
Cai, Qian
Ji, Debin
Jin, Seung-Gi
Niedernhofer, Laura J.
Pfeifer, Gerd P.
Xu, Guo-Liang
Wang, Yinsheng
author_sort Fu, Lijuan
collection PubMed
description [Image: see text] Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology.
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spelling pubmed-41404972015-07-29 Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA Fu, Lijuan Guerrero, Candace R. Zhong, Na Amato, Nicholas J. Liu, Yunhua Liu, Shuo Cai, Qian Ji, Debin Jin, Seung-Gi Niedernhofer, Laura J. Pfeifer, Gerd P. Xu, Guo-Liang Wang, Yinsheng J Am Chem Soc [Image: see text] Oxidation of 5-methylcytosine in DNA by ten-eleven translocation (Tet) family of enzymes has been demonstrated to play a significant role in epigenetic regulation in mammals. We found that Tet enzymes also possess the activity of catalyzing the formation of 5-hydroxymethylcytidine (5-hmrC) in RNA in vitro. In addition, the catalytic domains of all three Tet enzymes as well as full-length Tet3 could induce the formation of 5-hmrC in human cells. Moreover, 5-hmrC was present at appreciable levels (∼1 per 5000 5-methylcytidine) in RNA of mammalian cells and tissues. Our results suggest the involvement of this oxidation in RNA biology. American Chemical Society 2014-07-29 2014-08-20 /pmc/articles/PMC4140497/ /pubmed/25073028 http://dx.doi.org/10.1021/ja505305z Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Fu, Lijuan
Guerrero, Candace R.
Zhong, Na
Amato, Nicholas J.
Liu, Yunhua
Liu, Shuo
Cai, Qian
Ji, Debin
Jin, Seung-Gi
Niedernhofer, Laura J.
Pfeifer, Gerd P.
Xu, Guo-Liang
Wang, Yinsheng
Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA
title Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA
title_full Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA
title_fullStr Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA
title_full_unstemmed Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA
title_short Tet-Mediated Formation of 5-Hydroxymethylcytosine in RNA
title_sort tet-mediated formation of 5-hydroxymethylcytosine in rna
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140497/
https://www.ncbi.nlm.nih.gov/pubmed/25073028
http://dx.doi.org/10.1021/ja505305z
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