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Potential of Fluorescence Imaging Techniques To Monitor Mutagenic PAH Uptake by Microalga

[Image: see text] Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH), is one of the major environmental pollutants that causes mutagenesis and cancer. BaP has been shown to accumulate in phytoplankton and zooplankton. We have studied the localization and aggregation of BaP in Chlorella sp...

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Autores principales: Subashchandrabose, Suresh Ramraj, Krishnan, Kannan, Gratton, Enrico, Megharaj, Mallavarapu, Naidu, Ravi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140530/
https://www.ncbi.nlm.nih.gov/pubmed/25020149
http://dx.doi.org/10.1021/es500387v
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author Subashchandrabose, Suresh Ramraj
Krishnan, Kannan
Gratton, Enrico
Megharaj, Mallavarapu
Naidu, Ravi
author_facet Subashchandrabose, Suresh Ramraj
Krishnan, Kannan
Gratton, Enrico
Megharaj, Mallavarapu
Naidu, Ravi
author_sort Subashchandrabose, Suresh Ramraj
collection PubMed
description [Image: see text] Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH), is one of the major environmental pollutants that causes mutagenesis and cancer. BaP has been shown to accumulate in phytoplankton and zooplankton. We have studied the localization and aggregation of BaP in Chlorella sp., a microalga that is one of the primary producers in the food chain, using fluorescence confocal microscopy and fluorescence lifetime imaging microscopy with the phasor approach to characterize the location and the aggregation of BaP in the cell. Our results show that BaP accumulates in the lipid bodies of Chlorella sp. and that there is Förster resonance energy transfer between BaP and photosystems of Chlorella sp., indicating the close proximity of the two molecular systems. The lifetime of BaP fluorescence was measured to be 14 ns in N,N-dimethylformamide, an average of 7 ns in Bold’s basal medium, and 8 ns in Chlorella cells. Number and brightness analysis suggests that BaP does not aggregate inside Chlorella sp. (average brightness = 5.330), while it aggregates in the supernatant. In Chlorella grown in sediments spiked with BaP, in 12 h the BaP uptake could be visualized using fluorescence microscopy.
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spelling pubmed-41405302015-07-14 Potential of Fluorescence Imaging Techniques To Monitor Mutagenic PAH Uptake by Microalga Subashchandrabose, Suresh Ramraj Krishnan, Kannan Gratton, Enrico Megharaj, Mallavarapu Naidu, Ravi Environ Sci Technol [Image: see text] Benzo[a]pyrene (BaP), a polycyclic aromatic hydrocarbon (PAH), is one of the major environmental pollutants that causes mutagenesis and cancer. BaP has been shown to accumulate in phytoplankton and zooplankton. We have studied the localization and aggregation of BaP in Chlorella sp., a microalga that is one of the primary producers in the food chain, using fluorescence confocal microscopy and fluorescence lifetime imaging microscopy with the phasor approach to characterize the location and the aggregation of BaP in the cell. Our results show that BaP accumulates in the lipid bodies of Chlorella sp. and that there is Förster resonance energy transfer between BaP and photosystems of Chlorella sp., indicating the close proximity of the two molecular systems. The lifetime of BaP fluorescence was measured to be 14 ns in N,N-dimethylformamide, an average of 7 ns in Bold’s basal medium, and 8 ns in Chlorella cells. Number and brightness analysis suggests that BaP does not aggregate inside Chlorella sp. (average brightness = 5.330), while it aggregates in the supernatant. In Chlorella grown in sediments spiked with BaP, in 12 h the BaP uptake could be visualized using fluorescence microscopy. American Chemical Society 2014-07-14 2014-08-19 /pmc/articles/PMC4140530/ /pubmed/25020149 http://dx.doi.org/10.1021/es500387v Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Subashchandrabose, Suresh Ramraj
Krishnan, Kannan
Gratton, Enrico
Megharaj, Mallavarapu
Naidu, Ravi
Potential of Fluorescence Imaging Techniques To Monitor Mutagenic PAH Uptake by Microalga
title Potential of Fluorescence Imaging Techniques To Monitor Mutagenic PAH Uptake by Microalga
title_full Potential of Fluorescence Imaging Techniques To Monitor Mutagenic PAH Uptake by Microalga
title_fullStr Potential of Fluorescence Imaging Techniques To Monitor Mutagenic PAH Uptake by Microalga
title_full_unstemmed Potential of Fluorescence Imaging Techniques To Monitor Mutagenic PAH Uptake by Microalga
title_short Potential of Fluorescence Imaging Techniques To Monitor Mutagenic PAH Uptake by Microalga
title_sort potential of fluorescence imaging techniques to monitor mutagenic pah uptake by microalga
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140530/
https://www.ncbi.nlm.nih.gov/pubmed/25020149
http://dx.doi.org/10.1021/es500387v
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