Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Light sheet microscopy is the ideal imaging technique to study zebrafish embryonic development. Due to minimal photo-toxicity and bleaching, it is particularly suited for long-term time-lapse imaging over many hours up to several days. However, an appropriate sample mounting strategy is needed that...

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Detalles Bibliográficos
Autores principales: Weber, Michael, Mickoleit, Michaela, Huisken, Jan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2014
Materias:
Developmental Biology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140555/
https://www.ncbi.nlm.nih.gov/pubmed/24637614
http://dx.doi.org/10.3791/51119
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author Weber, Michael
Mickoleit, Michaela
Huisken, Jan
author_facet Weber, Michael
Mickoleit, Michaela
Huisken, Jan
author_sort Weber, Michael
collection PubMed
description Light sheet microscopy is the ideal imaging technique to study zebrafish embryonic development. Due to minimal photo-toxicity and bleaching, it is particularly suited for long-term time-lapse imaging over many hours up to several days. However, an appropriate sample mounting strategy is needed that offers both confinement and normal development of the sample. Multilayer mounting, a new embedding technique using low-concentration agarose in optically clear tubes, now overcomes this limitation and unleashes the full potential of light sheet microscopy for real-time developmental biology.
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spelling pubmed-41405552014-09-02 Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish Weber, Michael Mickoleit, Michaela Huisken, Jan J Vis Exp Developmental Biology Light sheet microscopy is the ideal imaging technique to study zebrafish embryonic development. Due to minimal photo-toxicity and bleaching, it is particularly suited for long-term time-lapse imaging over many hours up to several days. However, an appropriate sample mounting strategy is needed that offers both confinement and normal development of the sample. Multilayer mounting, a new embedding technique using low-concentration agarose in optically clear tubes, now overcomes this limitation and unleashes the full potential of light sheet microscopy for real-time developmental biology. MyJove Corporation 2014-02-27 /pmc/articles/PMC4140555/ /pubmed/24637614 http://dx.doi.org/10.3791/51119 Text en Copyright © 2014, Journal of Visualized Experiments http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. To view a copy of this license, visithttp://creativecommons.org/licenses/by-nc-nd/3.0/
spellingShingle Developmental Biology
Weber, Michael
Mickoleit, Michaela
Huisken, Jan
Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
title Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
title_full Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
title_fullStr Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
title_full_unstemmed Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
title_short Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
title_sort multilayer mounting for long-term light sheet microscopy of zebrafish
topic Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4140555/
https://www.ncbi.nlm.nih.gov/pubmed/24637614
http://dx.doi.org/10.3791/51119
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