Demethionylation of Pro-1 variants of 4-oxalocrotonate tautomerase in Escherichia coli by co-expression with an engineered methionine aminopeptidase

4-Oxalocrotonate tautomerase (4-OT) catalyzes the enol-keto tautomerization of 2-hydroxymuconate, utilizing its N-terminal proline (Pro-1) as general base catalyst. Substituting Pro-1 with bulky or charged residues will result in poor or no post-translational removal of the translation-initiating me...

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Detalles Bibliográficos
Autores principales: Baas, Bert-Jan, Zandvoort, Ellen, Wasiel, Anna A., Poelarends, Gerrit J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141196/
https://www.ncbi.nlm.nih.gov/pubmed/25161874
http://dx.doi.org/10.1016/j.fob.2014.07.003

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141196/
https://www.ncbi.nlm.nih.gov/pubmed/25161874
http://dx.doi.org/10.1016/j.fob.2014.07.003

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