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Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea

Dopamine (DA) receptor (DR) type 1 (D1R) has been found to be expressed in luteal cells of various species, but the intrinsic role of the DA/DRs system on corpora lutea (CL) function is still unclear. Experiments were devised to characterize the expression of DR types and the presence of DA, as well...

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Autores principales: Parillo, Francesco, Maranesi, Margherita, Mignini, Fiorenzo, Marinelli, Lisa, Di Stefano, Antonio, Boiti, Cristiano, Zerani, Massimo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141718/
https://www.ncbi.nlm.nih.gov/pubmed/25148384
http://dx.doi.org/10.1371/journal.pone.0104797
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author Parillo, Francesco
Maranesi, Margherita
Mignini, Fiorenzo
Marinelli, Lisa
Di Stefano, Antonio
Boiti, Cristiano
Zerani, Massimo
author_facet Parillo, Francesco
Maranesi, Margherita
Mignini, Fiorenzo
Marinelli, Lisa
Di Stefano, Antonio
Boiti, Cristiano
Zerani, Massimo
author_sort Parillo, Francesco
collection PubMed
description Dopamine (DA) receptor (DR) type 1 (D1R) has been found to be expressed in luteal cells of various species, but the intrinsic role of the DA/DRs system on corpora lutea (CL) function is still unclear. Experiments were devised to characterize the expression of DR types and the presence of DA, as well as the in vitro effects of DA on hormone productions by CL in pseudopregnant rabbits. Immunoreactivity and gene expression for D1R decreased while that for D3R increased in luteal and blood vessel cells from early to late pseudopregnant stages. DA immunopositivity was evidenced only in luteal cells. The DA and D1R agonist increased in vitro release of progesterone and prostaglandin E2 (PGE2) by early CL, whereas the DA and D3R agonist decreased progesterone and increased PGF2α in vitro release by mid- and late CL. These results provide evidence that the DA/DR system exerts a dual modulatory function in the lifespan of CL: the DA/D1R is luteotropic while the DA/D3R is luteolytic. The present data shed new light on the physiological mechanisms regulating luteal activity that might improve our ability to optimize reproductive efficiency in mammal species, including humans.
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spelling pubmed-41417182014-08-25 Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea Parillo, Francesco Maranesi, Margherita Mignini, Fiorenzo Marinelli, Lisa Di Stefano, Antonio Boiti, Cristiano Zerani, Massimo PLoS One Research Article Dopamine (DA) receptor (DR) type 1 (D1R) has been found to be expressed in luteal cells of various species, but the intrinsic role of the DA/DRs system on corpora lutea (CL) function is still unclear. Experiments were devised to characterize the expression of DR types and the presence of DA, as well as the in vitro effects of DA on hormone productions by CL in pseudopregnant rabbits. Immunoreactivity and gene expression for D1R decreased while that for D3R increased in luteal and blood vessel cells from early to late pseudopregnant stages. DA immunopositivity was evidenced only in luteal cells. The DA and D1R agonist increased in vitro release of progesterone and prostaglandin E2 (PGE2) by early CL, whereas the DA and D3R agonist decreased progesterone and increased PGF2α in vitro release by mid- and late CL. These results provide evidence that the DA/DR system exerts a dual modulatory function in the lifespan of CL: the DA/D1R is luteotropic while the DA/D3R is luteolytic. The present data shed new light on the physiological mechanisms regulating luteal activity that might improve our ability to optimize reproductive efficiency in mammal species, including humans. Public Library of Science 2014-08-22 /pmc/articles/PMC4141718/ /pubmed/25148384 http://dx.doi.org/10.1371/journal.pone.0104797 Text en © 2014 Parillo et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Parillo, Francesco
Maranesi, Margherita
Mignini, Fiorenzo
Marinelli, Lisa
Di Stefano, Antonio
Boiti, Cristiano
Zerani, Massimo
Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea
title Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea
title_full Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea
title_fullStr Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea
title_full_unstemmed Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea
title_short Evidence for a Dopamine Intrinsic Direct Role in the Regulation of the Ovary Reproductive Function: In Vitro Study on Rabbit Corpora Lutea
title_sort evidence for a dopamine intrinsic direct role in the regulation of the ovary reproductive function: in vitro study on rabbit corpora lutea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141718/
https://www.ncbi.nlm.nih.gov/pubmed/25148384
http://dx.doi.org/10.1371/journal.pone.0104797
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