Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas

Despite its many favorable properties as a sample support for biological electron microscopy, graphene is not widely used because its hydrophobicity precludes reliable protein deposition. We describe a method to modify graphene using a low-energy hydrogen plasma, which reduces hydrophobicity without...

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Detalles Bibliográficos
Autores principales: Russo, Christopher J., Passmore, Lori A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2014
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141966/
https://www.ncbi.nlm.nih.gov/pubmed/24747813
http://dx.doi.org/10.1038/nmeth.2931
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author Russo, Christopher J.
Passmore, Lori A.
author_facet Russo, Christopher J.
Passmore, Lori A.
author_sort Russo, Christopher J.
collection PubMed
description Despite its many favorable properties as a sample support for biological electron microscopy, graphene is not widely used because its hydrophobicity precludes reliable protein deposition. We describe a method to modify graphene using a low-energy hydrogen plasma, which reduces hydrophobicity without degrading the graphene lattice. We show that the use of plasma-treated graphene enables better control of protein distribution in ice for electron cryo-microscopy and improved image quality by reducing radiation-induced sample motion.
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spelling pubmed-41419662014-12-01 Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas Russo, Christopher J. Passmore, Lori A. Nat Methods Article Despite its many favorable properties as a sample support for biological electron microscopy, graphene is not widely used because its hydrophobicity precludes reliable protein deposition. We describe a method to modify graphene using a low-energy hydrogen plasma, which reduces hydrophobicity without degrading the graphene lattice. We show that the use of plasma-treated graphene enables better control of protein distribution in ice for electron cryo-microscopy and improved image quality by reducing radiation-induced sample motion. 2014-04-20 2014-06 /pmc/articles/PMC4141966/ /pubmed/24747813 http://dx.doi.org/10.1038/nmeth.2931 Text en Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Russo, Christopher J.
Passmore, Lori A.
Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas
title Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas
title_full Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas
title_fullStr Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas
title_full_unstemmed Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas
title_short Controlling protein adsorption on graphene for cryo-EM using low-energy hydrogen plasmas
title_sort controlling protein adsorption on graphene for cryo-em using low-energy hydrogen plasmas
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4141966/
https://www.ncbi.nlm.nih.gov/pubmed/24747813
http://dx.doi.org/10.1038/nmeth.2931
work_keys_str_mv AT russochristopherj controllingproteinadsorptionongrapheneforcryoemusinglowenergyhydrogenplasmas
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