Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water

We isolated a bacterial isolate (F7) from potable water. The strain was identified as Mesorhizobium sp. by 16S rDNA gene phylogenetic analysis and screened for N-acyl homoserine lactone (AHL) production by an AHL biosensor. The AHL profile of the isolate was further analyzed using high resolution tr...

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Detalles Bibliográficos
Autores principales: Yong, Pei-Ling, Chan, Kok-Gan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142172/
https://www.ncbi.nlm.nih.gov/pubmed/25177734
http://dx.doi.org/10.1155/2014/874764
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author Yong, Pei-Ling
Chan, Kok-Gan
author_facet Yong, Pei-Ling
Chan, Kok-Gan
author_sort Yong, Pei-Ling
collection PubMed
description We isolated a bacterial isolate (F7) from potable water. The strain was identified as Mesorhizobium sp. by 16S rDNA gene phylogenetic analysis and screened for N-acyl homoserine lactone (AHL) production by an AHL biosensor. The AHL profile of the isolate was further analyzed using high resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) which confirmed the production of multiple AHLs, namely, N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8-HSL) and N-3-oxo-decanoyl-L-homoserine lactone (3-oxo-C10-HSL). These findings will open the perspective to study the function of these AHLs in plant-microbe interactions.
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spelling pubmed-41421722014-08-31 Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water Yong, Pei-Ling Chan, Kok-Gan ScientificWorldJournal Research Article We isolated a bacterial isolate (F7) from potable water. The strain was identified as Mesorhizobium sp. by 16S rDNA gene phylogenetic analysis and screened for N-acyl homoserine lactone (AHL) production by an AHL biosensor. The AHL profile of the isolate was further analyzed using high resolution triple quadrupole liquid chromatography mass spectrometry (LC/MS) which confirmed the production of multiple AHLs, namely, N-3-oxo-octanoyl-L-homoserine lactone (3-oxo-C8-HSL) and N-3-oxo-decanoyl-L-homoserine lactone (3-oxo-C10-HSL). These findings will open the perspective to study the function of these AHLs in plant-microbe interactions. Hindawi Publishing Corporation 2014 2014-08-06 /pmc/articles/PMC4142172/ /pubmed/25177734 http://dx.doi.org/10.1155/2014/874764 Text en Copyright © 2014 P.-L. Yong and K.-G. Chan. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Yong, Pei-Ling
Chan, Kok-Gan
Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
title Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
title_full Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
title_fullStr Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
title_full_unstemmed Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
title_short Quorum Sensing Activity of Mesorhizobium sp. F7 Isolated from Potable Water
title_sort quorum sensing activity of mesorhizobium sp. f7 isolated from potable water
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142172/
https://www.ncbi.nlm.nih.gov/pubmed/25177734
http://dx.doi.org/10.1155/2014/874764
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