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P2Y(12) Receptor on the Verge of a Neuroinflammatory Breakdown

In the CNS, neuroinflammation occurring during pathologies as amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS) is the consequence of an intricate interplay orchestrated by various cell phenotypes. Among the molecular cues having a role in this process, extracellular nucleotides are re...

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Autores principales: Amadio, Susanna, Parisi, Chiara, Montilli, Cinzia, Carrubba, Alberto Savio, Apolloni, Savina, Volonté, Cinzia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142314/
https://www.ncbi.nlm.nih.gov/pubmed/25180027
http://dx.doi.org/10.1155/2014/975849
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author Amadio, Susanna
Parisi, Chiara
Montilli, Cinzia
Carrubba, Alberto Savio
Apolloni, Savina
Volonté, Cinzia
author_facet Amadio, Susanna
Parisi, Chiara
Montilli, Cinzia
Carrubba, Alberto Savio
Apolloni, Savina
Volonté, Cinzia
author_sort Amadio, Susanna
collection PubMed
description In the CNS, neuroinflammation occurring during pathologies as amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS) is the consequence of an intricate interplay orchestrated by various cell phenotypes. Among the molecular cues having a role in this process, extracellular nucleotides are responsible for intercellular communication and propagation of inflammatory stimuli. This occurs by binding to several receptor subtypes, defined P2X/P2Y, which are widespread in different tissues and simultaneously localized on multiple cells. For instance, the metabotropic P2Y(12) subtype is found in the CNS on microglia, affecting activation and chemotaxis, on oligodendrocytes, possessing a hypothesized role in myelination, and on astrocytes. By comparative analysis, we have established here that P2Y(12) receptor immunolabelled by antibodies against C-terminus or second intracellular loop, is, respectively, distributed and modulated under neuroinflammatory conditions on ramified microglia or myelinated fibers, in primary organotypic cerebellar cultures, tissue slices from rat striatum and cerebellum, spinal cord sections from symptomatic/end stage SOD1-G93A ALS mice, and finally autoptic cortical tissue from progressive MS donors. We suggest that modulation of P2Y(12) expression might play a dual role as analytic marker of branched/surveillant microglia and demyelinating lesions, thus potentially acquiring a predictive value under neuroinflammatory conditions as those found in ALS and MS.
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spelling pubmed-41423142014-09-01 P2Y(12) Receptor on the Verge of a Neuroinflammatory Breakdown Amadio, Susanna Parisi, Chiara Montilli, Cinzia Carrubba, Alberto Savio Apolloni, Savina Volonté, Cinzia Mediators Inflamm Research Article In the CNS, neuroinflammation occurring during pathologies as amyotrophic lateral sclerosis (ALS) and multiple sclerosis (MS) is the consequence of an intricate interplay orchestrated by various cell phenotypes. Among the molecular cues having a role in this process, extracellular nucleotides are responsible for intercellular communication and propagation of inflammatory stimuli. This occurs by binding to several receptor subtypes, defined P2X/P2Y, which are widespread in different tissues and simultaneously localized on multiple cells. For instance, the metabotropic P2Y(12) subtype is found in the CNS on microglia, affecting activation and chemotaxis, on oligodendrocytes, possessing a hypothesized role in myelination, and on astrocytes. By comparative analysis, we have established here that P2Y(12) receptor immunolabelled by antibodies against C-terminus or second intracellular loop, is, respectively, distributed and modulated under neuroinflammatory conditions on ramified microglia or myelinated fibers, in primary organotypic cerebellar cultures, tissue slices from rat striatum and cerebellum, spinal cord sections from symptomatic/end stage SOD1-G93A ALS mice, and finally autoptic cortical tissue from progressive MS donors. We suggest that modulation of P2Y(12) expression might play a dual role as analytic marker of branched/surveillant microglia and demyelinating lesions, thus potentially acquiring a predictive value under neuroinflammatory conditions as those found in ALS and MS. Hindawi Publishing Corporation 2014 2014-08-07 /pmc/articles/PMC4142314/ /pubmed/25180027 http://dx.doi.org/10.1155/2014/975849 Text en Copyright © 2014 Susanna Amadio et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Amadio, Susanna
Parisi, Chiara
Montilli, Cinzia
Carrubba, Alberto Savio
Apolloni, Savina
Volonté, Cinzia
P2Y(12) Receptor on the Verge of a Neuroinflammatory Breakdown
title P2Y(12) Receptor on the Verge of a Neuroinflammatory Breakdown
title_full P2Y(12) Receptor on the Verge of a Neuroinflammatory Breakdown
title_fullStr P2Y(12) Receptor on the Verge of a Neuroinflammatory Breakdown
title_full_unstemmed P2Y(12) Receptor on the Verge of a Neuroinflammatory Breakdown
title_short P2Y(12) Receptor on the Verge of a Neuroinflammatory Breakdown
title_sort p2y(12) receptor on the verge of a neuroinflammatory breakdown
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142314/
https://www.ncbi.nlm.nih.gov/pubmed/25180027
http://dx.doi.org/10.1155/2014/975849
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