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Mathematical model with spatially uniform regulation explains long-range bidirectional transport of early endosomes in fungal hyphae

In many cellular contexts, cargo is transported bidirectionally along microtubule bundles by dynein and kinesin-family motors. Upstream factors influence how individual cargoes are locally regulated, as well as how long-range transport is regulated at the whole-cell scale. Although the details of lo...

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Detalles Bibliográficos
Autores principales: Gou, Jia, Edelstein-Keshet, Leah, Allard, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142613/
https://www.ncbi.nlm.nih.gov/pubmed/24943842
http://dx.doi.org/10.1091/mbc.E14-03-0826
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author Gou, Jia
Edelstein-Keshet, Leah
Allard, Jun
author_facet Gou, Jia
Edelstein-Keshet, Leah
Allard, Jun
author_sort Gou, Jia
collection PubMed
description In many cellular contexts, cargo is transported bidirectionally along microtubule bundles by dynein and kinesin-family motors. Upstream factors influence how individual cargoes are locally regulated, as well as how long-range transport is regulated at the whole-cell scale. Although the details of local, single-cargo bidirectional switching have been extensively studied, it remains to be elucidated how this results in cell-scale spatial organization. Here we develop a mathematical model of early endosome transport in Ustilago maydis. We demonstrate that spatiotemporally uniform regulation, with constant transition rates, results in cargo dynamics that is consistent with experimental data, including data from motor mutants. We find that microtubule arrays can be symmetric in plus-end distribution but asymmetric in binding-site distribution in a manner that affects cargo dynamics and that cargo can travel past microtubule ends in microtubule bundles. Our model makes several testable predictions, including secondary features of dynein and cargo distributions.
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spelling pubmed-41426132014-10-30 Mathematical model with spatially uniform regulation explains long-range bidirectional transport of early endosomes in fungal hyphae Gou, Jia Edelstein-Keshet, Leah Allard, Jun Mol Biol Cell Articles In many cellular contexts, cargo is transported bidirectionally along microtubule bundles by dynein and kinesin-family motors. Upstream factors influence how individual cargoes are locally regulated, as well as how long-range transport is regulated at the whole-cell scale. Although the details of local, single-cargo bidirectional switching have been extensively studied, it remains to be elucidated how this results in cell-scale spatial organization. Here we develop a mathematical model of early endosome transport in Ustilago maydis. We demonstrate that spatiotemporally uniform regulation, with constant transition rates, results in cargo dynamics that is consistent with experimental data, including data from motor mutants. We find that microtubule arrays can be symmetric in plus-end distribution but asymmetric in binding-site distribution in a manner that affects cargo dynamics and that cargo can travel past microtubule ends in microtubule bundles. Our model makes several testable predictions, including secondary features of dynein and cargo distributions. The American Society for Cell Biology 2014-08-15 /pmc/articles/PMC4142613/ /pubmed/24943842 http://dx.doi.org/10.1091/mbc.E14-03-0826 Text en © 2014 Gou et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Gou, Jia
Edelstein-Keshet, Leah
Allard, Jun
Mathematical model with spatially uniform regulation explains long-range bidirectional transport of early endosomes in fungal hyphae
title Mathematical model with spatially uniform regulation explains long-range bidirectional transport of early endosomes in fungal hyphae
title_full Mathematical model with spatially uniform regulation explains long-range bidirectional transport of early endosomes in fungal hyphae
title_fullStr Mathematical model with spatially uniform regulation explains long-range bidirectional transport of early endosomes in fungal hyphae
title_full_unstemmed Mathematical model with spatially uniform regulation explains long-range bidirectional transport of early endosomes in fungal hyphae
title_short Mathematical model with spatially uniform regulation explains long-range bidirectional transport of early endosomes in fungal hyphae
title_sort mathematical model with spatially uniform regulation explains long-range bidirectional transport of early endosomes in fungal hyphae
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142613/
https://www.ncbi.nlm.nih.gov/pubmed/24943842
http://dx.doi.org/10.1091/mbc.E14-03-0826
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