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Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line
BACKGROUND: Adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines. It has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer. This...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cancer Research Center, Shahid Beheshti University of Medical Sciences
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142930/ https://www.ncbi.nlm.nih.gov/pubmed/25250128 |
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author | Gorgian Mohammadi, Masumeh Hedayati, Mehdi Zarghami, Nosratollah Ghaemmaghami, Sara Mohaddes, Mojtaba |
author_facet | Gorgian Mohammadi, Masumeh Hedayati, Mehdi Zarghami, Nosratollah Ghaemmaghami, Sara Mohaddes, Mojtaba |
author_sort | Gorgian Mohammadi, Masumeh |
collection | PubMed |
description | BACKGROUND: Adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines. It has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer. This study has managed to investigate endogenous and/or exogenous expression of Visfatin and Resistin in gastric cancer cell line. METHODS: Cell culture and semi-quantitative reverse transcription polymerase chain reaction has performed to measure mRNA and protein expression of Resistin and Visfatin in gastric cancer cell lines. ELISA test has performed for cell lysate and supernatant of cell culture to measure Resistin and Visfatin protein expression and secretion. RESULTS: Human gastric cancer cell line (AGS cell line) has found to express Visfatin mRNA and protein but Resistin mRNA and protein has not expressed. CONCLUSION: Visfatin has expressed endogenously in AGS human gastric cancer cells. Conversely Resistin has no expression. The results of this study has suggested that expression of adipocytokine proteins in real samples, could be a biomarker for gastric cancer. |
format | Online Article Text |
id | pubmed-4142930 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Cancer Research Center, Shahid Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-41429302014-09-23 Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line Gorgian Mohammadi, Masumeh Hedayati, Mehdi Zarghami, Nosratollah Ghaemmaghami, Sara Mohaddes, Mojtaba Iran J Cancer Prev Original Article BACKGROUND: Adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines. It has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer. This study has managed to investigate endogenous and/or exogenous expression of Visfatin and Resistin in gastric cancer cell line. METHODS: Cell culture and semi-quantitative reverse transcription polymerase chain reaction has performed to measure mRNA and protein expression of Resistin and Visfatin in gastric cancer cell lines. ELISA test has performed for cell lysate and supernatant of cell culture to measure Resistin and Visfatin protein expression and secretion. RESULTS: Human gastric cancer cell line (AGS cell line) has found to express Visfatin mRNA and protein but Resistin mRNA and protein has not expressed. CONCLUSION: Visfatin has expressed endogenously in AGS human gastric cancer cells. Conversely Resistin has no expression. The results of this study has suggested that expression of adipocytokine proteins in real samples, could be a biomarker for gastric cancer. Cancer Research Center, Shahid Beheshti University of Medical Sciences 2013 /pmc/articles/PMC4142930/ /pubmed/25250128 Text en © 2014 Cancer Research Center, Shahid Beheshti University of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0/ This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. |
spellingShingle | Original Article Gorgian Mohammadi, Masumeh Hedayati, Mehdi Zarghami, Nosratollah Ghaemmaghami, Sara Mohaddes, Mojtaba Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line |
title | Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line |
title_full | Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line |
title_fullStr | Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line |
title_full_unstemmed | Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line |
title_short | Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line |
title_sort | adipocyte derived hormones gene expression, resistin and visfatin, in ags gastric cancer cell line |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4142930/ https://www.ncbi.nlm.nih.gov/pubmed/25250128 |
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