Cargando…

Cell Patterning on Photolithographically Defined Parylene-C: SiO(2) Substrates

Cell patterning platforms support broad research goals, such as construction of predefined in vitro neuronal networks and the exploration of certain central aspects of cellular physiology. To easily combine cell patterning with Multi-Electrode Arrays (MEAs) and silicon-based ‘lab on a chip’ technolo...

Descripción completa

Detalles Bibliográficos
Autores principales: Hughes, Mark A., Brennan, Paul M., Bunting, Andrew S., Shipston, Mike J., Murray, Alan F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MyJove Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143168/
https://www.ncbi.nlm.nih.gov/pubmed/24637580
http://dx.doi.org/10.3791/50929
_version_ 1782331857855053824
author Hughes, Mark A.
Brennan, Paul M.
Bunting, Andrew S.
Shipston, Mike J.
Murray, Alan F.
author_facet Hughes, Mark A.
Brennan, Paul M.
Bunting, Andrew S.
Shipston, Mike J.
Murray, Alan F.
author_sort Hughes, Mark A.
collection PubMed
description Cell patterning platforms support broad research goals, such as construction of predefined in vitro neuronal networks and the exploration of certain central aspects of cellular physiology. To easily combine cell patterning with Multi-Electrode Arrays (MEAs) and silicon-based ‘lab on a chip’ technologies, a microfabrication-compatible protocol is required. We describe a method that utilizes deposition of the polymer parylene-C on SiO(2 )wafers. Photolithography enables accurate and reliable patterning of parylene-C at micron-level resolution. Subsequent activation by immersion in fetal bovine serum (or another specific activation solution) results in a substrate in which cultured cells adhere to, or are repulsed by, parylene or SiO(2) regions respectively. This technique has allowed patterning of a broad range of cell types (including primary murine hippocampal cells, HEK 293 cell line, human neuron-like teratocarcinoma cell line, primary murine cerebellar granule cells, and primary human glioma-derived stem-like cells). Interestingly, however, the platform is not universal; reflecting the importance of cell-specific adhesion molecules. This cell patterning process is cost effective, reliable, and importantly can be incorporated into standard microfabrication (chip manufacturing) protocols, paving the way for integration of microelectronic technology.
format Online
Article
Text
id pubmed-4143168
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher MyJove Corporation
record_format MEDLINE/PubMed
spelling pubmed-41431682014-08-27 Cell Patterning on Photolithographically Defined Parylene-C: SiO(2) Substrates Hughes, Mark A. Brennan, Paul M. Bunting, Andrew S. Shipston, Mike J. Murray, Alan F. J Vis Exp Bioengineering Cell patterning platforms support broad research goals, such as construction of predefined in vitro neuronal networks and the exploration of certain central aspects of cellular physiology. To easily combine cell patterning with Multi-Electrode Arrays (MEAs) and silicon-based ‘lab on a chip’ technologies, a microfabrication-compatible protocol is required. We describe a method that utilizes deposition of the polymer parylene-C on SiO(2 )wafers. Photolithography enables accurate and reliable patterning of parylene-C at micron-level resolution. Subsequent activation by immersion in fetal bovine serum (or another specific activation solution) results in a substrate in which cultured cells adhere to, or are repulsed by, parylene or SiO(2) regions respectively. This technique has allowed patterning of a broad range of cell types (including primary murine hippocampal cells, HEK 293 cell line, human neuron-like teratocarcinoma cell line, primary murine cerebellar granule cells, and primary human glioma-derived stem-like cells). Interestingly, however, the platform is not universal; reflecting the importance of cell-specific adhesion molecules. This cell patterning process is cost effective, reliable, and importantly can be incorporated into standard microfabrication (chip manufacturing) protocols, paving the way for integration of microelectronic technology. MyJove Corporation 2014-03-07 /pmc/articles/PMC4143168/ /pubmed/24637580 http://dx.doi.org/10.3791/50929 Text en Copyright © 2014, Journal of Visualized Experiments http://creativecommons.org/licenses/by/3.0/us/ This is an open-access article distributed under the terms of the Creative Commons Attribution 3.0 License. To view a copy of this license, visithttp://creativecommons.org/licenses/by/3.0/us/
spellingShingle Bioengineering
Hughes, Mark A.
Brennan, Paul M.
Bunting, Andrew S.
Shipston, Mike J.
Murray, Alan F.
Cell Patterning on Photolithographically Defined Parylene-C: SiO(2) Substrates
title Cell Patterning on Photolithographically Defined Parylene-C: SiO(2) Substrates
title_full Cell Patterning on Photolithographically Defined Parylene-C: SiO(2) Substrates
title_fullStr Cell Patterning on Photolithographically Defined Parylene-C: SiO(2) Substrates
title_full_unstemmed Cell Patterning on Photolithographically Defined Parylene-C: SiO(2) Substrates
title_short Cell Patterning on Photolithographically Defined Parylene-C: SiO(2) Substrates
title_sort cell patterning on photolithographically defined parylene-c: sio(2) substrates
topic Bioengineering
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143168/
https://www.ncbi.nlm.nih.gov/pubmed/24637580
http://dx.doi.org/10.3791/50929
work_keys_str_mv AT hughesmarka cellpatterningonphotolithographicallydefinedparylenecsio2substrates
AT brennanpaulm cellpatterningonphotolithographicallydefinedparylenecsio2substrates
AT buntingandrews cellpatterningonphotolithographicallydefinedparylenecsio2substrates
AT shipstonmikej cellpatterningonphotolithographicallydefinedparylenecsio2substrates
AT murrayalanf cellpatterningonphotolithographicallydefinedparylenecsio2substrates