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Immunogenicity of an Electron Beam Inactivated Rhodococcus equi Vaccine in Neonatal Foals
Rhodococcus equi is an important pathogen of foals that causes severe pneumonia. To date, there is no licensed vaccine effective against R. equi pneumonia of foals. The objectives of our study were to develop an electron beam (eBeam) inactivated vaccine against R. equi and evaluate its immunogenicit...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143214/ https://www.ncbi.nlm.nih.gov/pubmed/25153708 http://dx.doi.org/10.1371/journal.pone.0105367 |
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author | Bordin, Angela I. Pillai, Suresh D. Brake, Courtney Bagley, Kaytee B. Bourquin, Jessica R. Coleman, Michelle Oliveira, Fabiano N. Mwangi, Waithaka McMurray, David N. Love, Charles C. Felippe, Maria Julia B. Cohen, Noah D. |
author_facet | Bordin, Angela I. Pillai, Suresh D. Brake, Courtney Bagley, Kaytee B. Bourquin, Jessica R. Coleman, Michelle Oliveira, Fabiano N. Mwangi, Waithaka McMurray, David N. Love, Charles C. Felippe, Maria Julia B. Cohen, Noah D. |
author_sort | Bordin, Angela I. |
collection | PubMed |
description | Rhodococcus equi is an important pathogen of foals that causes severe pneumonia. To date, there is no licensed vaccine effective against R. equi pneumonia of foals. The objectives of our study were to develop an electron beam (eBeam) inactivated vaccine against R. equi and evaluate its immunogenicity. A dose of eBeam irradiation that inactivated replication of R. equi while maintaining outer cell wall integrity was identified. Enteral administration of eBeam inactivated R. equi increased interferon-γ production by peripheral blood mononuclear cells in response to stimulation with virulent R. equi and generated naso-pharyngeal R. equi-specific IgA in newborn foals. Our results indicate that eBeam irradiated R. equi administered enterally produce cell-mediated and upper respiratory mucosal immune responses, in the face of passively transferred maternal antibodies, similar to those produced in response to enteral administration of live organisms (a strategy which previously has been documented to protect foals against intrabronchial infection with virulent R. equi). No evidence of adverse effects was noted among vaccinated foals. |
format | Online Article Text |
id | pubmed-4143214 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-41432142014-08-27 Immunogenicity of an Electron Beam Inactivated Rhodococcus equi Vaccine in Neonatal Foals Bordin, Angela I. Pillai, Suresh D. Brake, Courtney Bagley, Kaytee B. Bourquin, Jessica R. Coleman, Michelle Oliveira, Fabiano N. Mwangi, Waithaka McMurray, David N. Love, Charles C. Felippe, Maria Julia B. Cohen, Noah D. PLoS One Research Article Rhodococcus equi is an important pathogen of foals that causes severe pneumonia. To date, there is no licensed vaccine effective against R. equi pneumonia of foals. The objectives of our study were to develop an electron beam (eBeam) inactivated vaccine against R. equi and evaluate its immunogenicity. A dose of eBeam irradiation that inactivated replication of R. equi while maintaining outer cell wall integrity was identified. Enteral administration of eBeam inactivated R. equi increased interferon-γ production by peripheral blood mononuclear cells in response to stimulation with virulent R. equi and generated naso-pharyngeal R. equi-specific IgA in newborn foals. Our results indicate that eBeam irradiated R. equi administered enterally produce cell-mediated and upper respiratory mucosal immune responses, in the face of passively transferred maternal antibodies, similar to those produced in response to enteral administration of live organisms (a strategy which previously has been documented to protect foals against intrabronchial infection with virulent R. equi). No evidence of adverse effects was noted among vaccinated foals. Public Library of Science 2014-08-25 /pmc/articles/PMC4143214/ /pubmed/25153708 http://dx.doi.org/10.1371/journal.pone.0105367 Text en © 2014 Bordin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Bordin, Angela I. Pillai, Suresh D. Brake, Courtney Bagley, Kaytee B. Bourquin, Jessica R. Coleman, Michelle Oliveira, Fabiano N. Mwangi, Waithaka McMurray, David N. Love, Charles C. Felippe, Maria Julia B. Cohen, Noah D. Immunogenicity of an Electron Beam Inactivated Rhodococcus equi Vaccine in Neonatal Foals |
title | Immunogenicity of an Electron Beam Inactivated Rhodococcus equi Vaccine in Neonatal Foals |
title_full | Immunogenicity of an Electron Beam Inactivated Rhodococcus equi Vaccine in Neonatal Foals |
title_fullStr | Immunogenicity of an Electron Beam Inactivated Rhodococcus equi Vaccine in Neonatal Foals |
title_full_unstemmed | Immunogenicity of an Electron Beam Inactivated Rhodococcus equi Vaccine in Neonatal Foals |
title_short | Immunogenicity of an Electron Beam Inactivated Rhodococcus equi Vaccine in Neonatal Foals |
title_sort | immunogenicity of an electron beam inactivated rhodococcus equi vaccine in neonatal foals |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143214/ https://www.ncbi.nlm.nih.gov/pubmed/25153708 http://dx.doi.org/10.1371/journal.pone.0105367 |
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