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Metallization and Biopatterning on Ultra-Flexible Substrates via Dextran Sacrificial Layers

Micro-patterning tools adopted from the semiconductor industry have mostly been optimized to pattern features onto rigid silicon and glass substrates, however, recently the need to pattern on soft substrates has been identified in simulating cellular environments or developing flexible biosensors. W...

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Detalles Bibliográficos
Autores principales: Tseng, Peter, Pushkarsky, Ivan, Di Carlo, Dino
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143360/
https://www.ncbi.nlm.nih.gov/pubmed/25153326
http://dx.doi.org/10.1371/journal.pone.0106091
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author Tseng, Peter
Pushkarsky, Ivan
Di Carlo, Dino
author_facet Tseng, Peter
Pushkarsky, Ivan
Di Carlo, Dino
author_sort Tseng, Peter
collection PubMed
description Micro-patterning tools adopted from the semiconductor industry have mostly been optimized to pattern features onto rigid silicon and glass substrates, however, recently the need to pattern on soft substrates has been identified in simulating cellular environments or developing flexible biosensors. We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films. Dextran films provided a stable template for photolithography, metal deposition, particle adsorption, and protein stamping. These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water. We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives. This method is compatible over the complete moduli range of PDMS, and potentially generalizable over a host of additional micro- and nano-structures and materials.
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spelling pubmed-41433602014-08-27 Metallization and Biopatterning on Ultra-Flexible Substrates via Dextran Sacrificial Layers Tseng, Peter Pushkarsky, Ivan Di Carlo, Dino PLoS One Research Article Micro-patterning tools adopted from the semiconductor industry have mostly been optimized to pattern features onto rigid silicon and glass substrates, however, recently the need to pattern on soft substrates has been identified in simulating cellular environments or developing flexible biosensors. We present a simple method of introducing a variety of patterned materials and structures into ultra-flexible polydimethylsiloxane (PDMS) layers (elastic moduli down to 3 kPa) utilizing water-soluble dextran sacrificial thin films. Dextran films provided a stable template for photolithography, metal deposition, particle adsorption, and protein stamping. These materials and structures (including dextran itself) were then readily transferrable to an elastomer surface following PDMS (10 to 70∶1 base to crosslinker ratios) curing over the patterned dextran layer and after sacrificial etch of the dextran in water. We demonstrate that this simple and straightforward approach can controllably manipulate surface wetting and protein adsorption characteristics of PDMS, covalently link protein patterns for stable cell patterning, generate composite structures of epoxy or particles for study of cell mechanical response, and stably integrate certain metals with use of vinyl molecular adhesives. This method is compatible over the complete moduli range of PDMS, and potentially generalizable over a host of additional micro- and nano-structures and materials. Public Library of Science 2014-08-25 /pmc/articles/PMC4143360/ /pubmed/25153326 http://dx.doi.org/10.1371/journal.pone.0106091 Text en © 2014 Tseng et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tseng, Peter
Pushkarsky, Ivan
Di Carlo, Dino
Metallization and Biopatterning on Ultra-Flexible Substrates via Dextran Sacrificial Layers
title Metallization and Biopatterning on Ultra-Flexible Substrates via Dextran Sacrificial Layers
title_full Metallization and Biopatterning on Ultra-Flexible Substrates via Dextran Sacrificial Layers
title_fullStr Metallization and Biopatterning on Ultra-Flexible Substrates via Dextran Sacrificial Layers
title_full_unstemmed Metallization and Biopatterning on Ultra-Flexible Substrates via Dextran Sacrificial Layers
title_short Metallization and Biopatterning on Ultra-Flexible Substrates via Dextran Sacrificial Layers
title_sort metallization and biopatterning on ultra-flexible substrates via dextran sacrificial layers
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143360/
https://www.ncbi.nlm.nih.gov/pubmed/25153326
http://dx.doi.org/10.1371/journal.pone.0106091
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