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Quantitative and pattern recognition analyses of magnoflorine, spinosin, 6′′′-feruloyl spinosin and jujuboside A by HPLC in Zizyphi Semen
Two rapid and simple HPLC methods with UV detector to determine three main compounds (magnoflorine, spinosin and 6′′′-feruloyl spinosin) and evaporative light scattering detector (ELSD) to determine jujuboside A were developed for the chemical analyses of Zizyphi Semen. Magnoflorine, spinosin, and 6...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Pharmaceutical Society of Korea
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143599/ https://www.ncbi.nlm.nih.gov/pubmed/24310099 http://dx.doi.org/10.1007/s12272-013-0295-z |
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author | Kim, Won Il Zhao, Bing Tian Zhang, Hai Yan Lee, Je Hyun Son, Jong Keun Woo, Mi Hee |
author_facet | Kim, Won Il Zhao, Bing Tian Zhang, Hai Yan Lee, Je Hyun Son, Jong Keun Woo, Mi Hee |
author_sort | Kim, Won Il |
collection | PubMed |
description | Two rapid and simple HPLC methods with UV detector to determine three main compounds (magnoflorine, spinosin and 6′′′-feruloyl spinosin) and evaporative light scattering detector (ELSD) to determine jujuboside A were developed for the chemical analyses of Zizyphi Semen. Magnoflorine, spinosin, and 6′′′-feruloyl spinosin were separated with an YMC J’sphere ODS-H80 column (250 mm × 4.6 mm, 4 μm) by the gradient elution followed by the isocratic elution using methanol with 0.1 % formic acid and water with 0.1 % formic acid as the mobile phase. The flow rate was 1.0 mL/min. Jujuboside A was separated by HPLC–ELSD with YoungJinBioChrom Aegispak C18-L column (250 mm × 4.6 mm, 5 μm) column in a gradient elution using methanol with 0.1 % formic acid (A) and water with 0.1 % formic acid as the mobile phase. These two methods were fully validated with respect to linearity, precision, accuracy, stability, and robustness. These HPLC methods were applied successfully to quantify four compounds in a Zizyphi Semen extract. The HPLC analytical methods were validated for pattern recognition analysis by repeated analysis of 91 seed samples corresponding to 48 Zizyphus jujuba var. spinosa (J01–J48) and 43 Zizyphus mauritiana (M01–M43). The results indicate that these methods are suitable for a quality evaluation of Zizyphi Semen. |
format | Online Article Text |
id | pubmed-4143599 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Pharmaceutical Society of Korea |
record_format | MEDLINE/PubMed |
spelling | pubmed-41435992014-08-26 Quantitative and pattern recognition analyses of magnoflorine, spinosin, 6′′′-feruloyl spinosin and jujuboside A by HPLC in Zizyphi Semen Kim, Won Il Zhao, Bing Tian Zhang, Hai Yan Lee, Je Hyun Son, Jong Keun Woo, Mi Hee Arch Pharm Res Research Article Two rapid and simple HPLC methods with UV detector to determine three main compounds (magnoflorine, spinosin and 6′′′-feruloyl spinosin) and evaporative light scattering detector (ELSD) to determine jujuboside A were developed for the chemical analyses of Zizyphi Semen. Magnoflorine, spinosin, and 6′′′-feruloyl spinosin were separated with an YMC J’sphere ODS-H80 column (250 mm × 4.6 mm, 4 μm) by the gradient elution followed by the isocratic elution using methanol with 0.1 % formic acid and water with 0.1 % formic acid as the mobile phase. The flow rate was 1.0 mL/min. Jujuboside A was separated by HPLC–ELSD with YoungJinBioChrom Aegispak C18-L column (250 mm × 4.6 mm, 5 μm) column in a gradient elution using methanol with 0.1 % formic acid (A) and water with 0.1 % formic acid as the mobile phase. These two methods were fully validated with respect to linearity, precision, accuracy, stability, and robustness. These HPLC methods were applied successfully to quantify four compounds in a Zizyphi Semen extract. The HPLC analytical methods were validated for pattern recognition analysis by repeated analysis of 91 seed samples corresponding to 48 Zizyphus jujuba var. spinosa (J01–J48) and 43 Zizyphus mauritiana (M01–M43). The results indicate that these methods are suitable for a quality evaluation of Zizyphi Semen. Pharmaceutical Society of Korea 2013-12-06 2014 /pmc/articles/PMC4143599/ /pubmed/24310099 http://dx.doi.org/10.1007/s12272-013-0295-z Text en © The Author(s) 2013 https://creativecommons.org/licenses/by/2.0/ Open AccessThis article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Research Article Kim, Won Il Zhao, Bing Tian Zhang, Hai Yan Lee, Je Hyun Son, Jong Keun Woo, Mi Hee Quantitative and pattern recognition analyses of magnoflorine, spinosin, 6′′′-feruloyl spinosin and jujuboside A by HPLC in Zizyphi Semen |
title | Quantitative and pattern recognition analyses of magnoflorine, spinosin, 6′′′-feruloyl spinosin and jujuboside A by HPLC in Zizyphi Semen |
title_full | Quantitative and pattern recognition analyses of magnoflorine, spinosin, 6′′′-feruloyl spinosin and jujuboside A by HPLC in Zizyphi Semen |
title_fullStr | Quantitative and pattern recognition analyses of magnoflorine, spinosin, 6′′′-feruloyl spinosin and jujuboside A by HPLC in Zizyphi Semen |
title_full_unstemmed | Quantitative and pattern recognition analyses of magnoflorine, spinosin, 6′′′-feruloyl spinosin and jujuboside A by HPLC in Zizyphi Semen |
title_short | Quantitative and pattern recognition analyses of magnoflorine, spinosin, 6′′′-feruloyl spinosin and jujuboside A by HPLC in Zizyphi Semen |
title_sort | quantitative and pattern recognition analyses of magnoflorine, spinosin, 6′′′-feruloyl spinosin and jujuboside a by hplc in zizyphi semen |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143599/ https://www.ncbi.nlm.nih.gov/pubmed/24310099 http://dx.doi.org/10.1007/s12272-013-0295-z |
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