High Genetic Diversity of Anaplasma marginale Detected from Philippine Cattle

A total of 658 cattle in 6 provinces in the Philippines were screened for Anaplasma marginale infection by using a diagnostic heat-shock operon (groEL) gene-PCR assay. The screening-positive samples were further tested using the major surface antigen protein 1a (Msp1a) gene-PCR assay. Screening PCR...

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Detalles Bibliográficos
Autores principales: YBAÑEZ, Adrian Patalinghug, YBAÑEZ, Rochelle Haidee D., CLAVERIA, Florencia G., CRUZ-FLORES, Mary Jane, XUENAN, Xuen, YOKOYAMA, Naoaki, INOKUMA, Hisashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2014
Materias:
Bacteriology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143641/
https://www.ncbi.nlm.nih.gov/pubmed/24717413
http://dx.doi.org/10.1292/jvms.13-0405
Descripción
Sumario:A total of 658 cattle in 6 provinces in the Philippines were screened for Anaplasma marginale infection by using a diagnostic heat-shock operon (groEL) gene-PCR assay. The screening-positive samples were further tested using the major surface antigen protein 1a (Msp1a) gene-PCR assay. Screening PCR results showed 130 cattle (19.8%) were positive for the A. marginale infection. Subsequent amplification using the Msp1a gene only showed 93 samples (14.1%) to be positive. In addition, 37 tandem-repeat structures, including 20 novel structures, and 41 distinct genotypes were identified. Interestingly, multiple infections of 4 different genotypes were also observed in A. marginale-infected cattle. The present study demonstrated the prevalence and characterization of diverse genotypes of A. marginale in the Philippine cattle.

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