IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa

The aim of study was to evaluate the effects of insulin-like growth factor I (IGF-I) on canine sperm function during cooled and freeze-thaw storage. Extenders supplemented with different IGF-I concentrations (0, 100 and 200 ng/ml) were added to canine spermatozoa, and the sperm samples were stored a...

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Detalles Bibliográficos
Autores principales: SHIN, Sang-Min, KIM, Suhee, HONG, Jin-Gi, KIM, Yong-Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2014
Materias:
Theriogenology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143651/
https://www.ncbi.nlm.nih.gov/pubmed/24717414
http://dx.doi.org/10.1292/jvms.14-0049
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author SHIN, Sang-Min
KIM, Suhee
HONG, Jin-Gi
KIM, Yong-Jun
author_facet SHIN, Sang-Min
KIM, Suhee
HONG, Jin-Gi
KIM, Yong-Jun
author_sort SHIN, Sang-Min
collection PubMed
description The aim of study was to evaluate the effects of insulin-like growth factor I (IGF-I) on canine sperm function during cooled and freeze-thaw storage. Extenders supplemented with different IGF-I concentrations (0, 100 and 200 ng/ml) were added to canine spermatozoa, and the sperm samples were stored at 4°C for 48 hr or freeze-thawed. Sperm motility, morphology, plasma-membrane integrity (PMI) and mitochondrial membrane potential (MMP) were evaluated. IGF-I had no effect on PMI or morphology during cooling and freeze-thawing. However, IGF-I alleviated the reduction in progressive motility and MMP caused by cooled storage and led to an improvement in MMP after freeze-thawing. In conclusion, IGF-I can be helpful to maintain progressive motility of canine spermatozoa during hypothermic storage via increased MMP.
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spelling pubmed-41436512014-08-26 IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa SHIN, Sang-Min KIM, Suhee HONG, Jin-Gi KIM, Yong-Jun J Vet Med Sci Theriogenology The aim of study was to evaluate the effects of insulin-like growth factor I (IGF-I) on canine sperm function during cooled and freeze-thaw storage. Extenders supplemented with different IGF-I concentrations (0, 100 and 200 ng/ml) were added to canine spermatozoa, and the sperm samples were stored at 4°C for 48 hr or freeze-thawed. Sperm motility, morphology, plasma-membrane integrity (PMI) and mitochondrial membrane potential (MMP) were evaluated. IGF-I had no effect on PMI or morphology during cooling and freeze-thawing. However, IGF-I alleviated the reduction in progressive motility and MMP caused by cooled storage and led to an improvement in MMP after freeze-thawing. In conclusion, IGF-I can be helpful to maintain progressive motility of canine spermatozoa during hypothermic storage via increased MMP. The Japanese Society of Veterinary Science 2014-04-09 2014-07 /pmc/articles/PMC4143651/ /pubmed/24717414 http://dx.doi.org/10.1292/jvms.14-0049 Text en ©2014 The Japanese Society of Veterinary Science http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License.
spellingShingle Theriogenology
SHIN, Sang-Min
KIM, Suhee
HONG, Jin-Gi
KIM, Yong-Jun
IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa
title IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa
title_full IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa
title_fullStr IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa
title_full_unstemmed IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa
title_short IGF-I Improves Mitochondrial Membrane Potential during Hypothermic Storage of Canine Spermatozoa
title_sort igf-i improves mitochondrial membrane potential during hypothermic storage of canine spermatozoa
topic Theriogenology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143651/
https://www.ncbi.nlm.nih.gov/pubmed/24717414
http://dx.doi.org/10.1292/jvms.14-0049
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AT kimyongjun igfiimprovesmitochondrialmembranepotentialduringhypothermicstorageofcaninespermatozoa

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