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Mis-splicing of the ABCC2 gene linked with Bt toxin resistance in Helicoverpa armigera

Toxins from the bacterium Bacillus thuringiensis (Bt) are used widely for insect control in sprays and transgenic plants, but their efficacy is reduced when pests evolve resistance. Previous work showed that mutations in a gene encoding the transporter protein ABCC2 are linked with resistance to Bt...

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Autores principales: Xiao, Yutao, Zhang, Tao, Liu, Chenxi, Heckel, David G., Li, Xianchun, Tabashnik, Bruce E., Wu, Kongming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143771/
https://www.ncbi.nlm.nih.gov/pubmed/25154974
http://dx.doi.org/10.1038/srep06184
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author Xiao, Yutao
Zhang, Tao
Liu, Chenxi
Heckel, David G.
Li, Xianchun
Tabashnik, Bruce E.
Wu, Kongming
author_facet Xiao, Yutao
Zhang, Tao
Liu, Chenxi
Heckel, David G.
Li, Xianchun
Tabashnik, Bruce E.
Wu, Kongming
author_sort Xiao, Yutao
collection PubMed
description Toxins from the bacterium Bacillus thuringiensis (Bt) are used widely for insect control in sprays and transgenic plants, but their efficacy is reduced when pests evolve resistance. Previous work showed that mutations in a gene encoding the transporter protein ABCC2 are linked with resistance to Bt toxins Cry1Ab, Cry1Ac or both in four species of Lepidoptera. Here we compared the ABCC2 gene of Helicoverpa armigera (HaABCC2) between susceptible strains and a laboratory-selected strain with >1,000-fold resistance to Cry1Ac relative its susceptible parent strain. We discovered a 73-base pair (bp) insertion in the cDNA of the resistant strain that generates a premature stop codon expected to yield a truncated ABCC2 protein. Sequencing of genomic DNA revealed that this insertion is an intron that is not spliced out because of a 6-bp deletion at its splicing site. Analysis of progeny from crosses revealed tight genetic linkage between HaABCC2 and resistance to Cry1Ac. These results provide the first evidence that mis-splicing of a gene encoding an ABCC2 protein confers resistance to a Bt toxin.
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spelling pubmed-41437712014-08-27 Mis-splicing of the ABCC2 gene linked with Bt toxin resistance in Helicoverpa armigera Xiao, Yutao Zhang, Tao Liu, Chenxi Heckel, David G. Li, Xianchun Tabashnik, Bruce E. Wu, Kongming Sci Rep Article Toxins from the bacterium Bacillus thuringiensis (Bt) are used widely for insect control in sprays and transgenic plants, but their efficacy is reduced when pests evolve resistance. Previous work showed that mutations in a gene encoding the transporter protein ABCC2 are linked with resistance to Bt toxins Cry1Ab, Cry1Ac or both in four species of Lepidoptera. Here we compared the ABCC2 gene of Helicoverpa armigera (HaABCC2) between susceptible strains and a laboratory-selected strain with >1,000-fold resistance to Cry1Ac relative its susceptible parent strain. We discovered a 73-base pair (bp) insertion in the cDNA of the resistant strain that generates a premature stop codon expected to yield a truncated ABCC2 protein. Sequencing of genomic DNA revealed that this insertion is an intron that is not spliced out because of a 6-bp deletion at its splicing site. Analysis of progeny from crosses revealed tight genetic linkage between HaABCC2 and resistance to Cry1Ac. These results provide the first evidence that mis-splicing of a gene encoding an ABCC2 protein confers resistance to a Bt toxin. Nature Publishing Group 2014-08-26 /pmc/articles/PMC4143771/ /pubmed/25154974 http://dx.doi.org/10.1038/srep06184 Text en Copyright © 2014, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/
spellingShingle Article
Xiao, Yutao
Zhang, Tao
Liu, Chenxi
Heckel, David G.
Li, Xianchun
Tabashnik, Bruce E.
Wu, Kongming
Mis-splicing of the ABCC2 gene linked with Bt toxin resistance in Helicoverpa armigera
title Mis-splicing of the ABCC2 gene linked with Bt toxin resistance in Helicoverpa armigera
title_full Mis-splicing of the ABCC2 gene linked with Bt toxin resistance in Helicoverpa armigera
title_fullStr Mis-splicing of the ABCC2 gene linked with Bt toxin resistance in Helicoverpa armigera
title_full_unstemmed Mis-splicing of the ABCC2 gene linked with Bt toxin resistance in Helicoverpa armigera
title_short Mis-splicing of the ABCC2 gene linked with Bt toxin resistance in Helicoverpa armigera
title_sort mis-splicing of the abcc2 gene linked with bt toxin resistance in helicoverpa armigera
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143771/
https://www.ncbi.nlm.nih.gov/pubmed/25154974
http://dx.doi.org/10.1038/srep06184
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