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Nanoscale Label-free Bioprobes to Detect Intracellular Proteins in Single Living Cells

Fluorescent labeling techniques have been widely used in live cell studies; however, the labeling processes can be laborious and challenging for use in non-transfectable cells, and labels can interfere with protein functions. While label-free biosensors have been realized by nanofabrication, a metho...

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Detalles Bibliográficos
Autores principales: Hong, Wooyoung, Liang, Feng, Schaak, Diane, Loncar, Marko, Quan, Qimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143788/
https://www.ncbi.nlm.nih.gov/pubmed/25154394
http://dx.doi.org/10.1038/srep06179
Descripción
Sumario:Fluorescent labeling techniques have been widely used in live cell studies; however, the labeling processes can be laborious and challenging for use in non-transfectable cells, and labels can interfere with protein functions. While label-free biosensors have been realized by nanofabrication, a method to track intracellular protein dynamics in real-time, in situ and in living cells has not been found. Here we present the first demonstration of label-free detection of intracellular p53 protein dynamics through a nanoscale surface plasmon-polariton fiber-tip-probe (FTP).