Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts

Leptospirosis is an important waterborne zoonotic disease caused by pathogenic Leptospira. The pathogen is maintained in a population due to chronic colonization and shedding from renal tubules of domestic and wild animals. Humans and other animals become infected when they come in contact with urin...

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Autores principales: Rawlins, Julienne, Portanova, Alexandra, Zuckerman, Ilana, Loftis, Amanda, Ceccato, Pietro, Willingham, Arve Lee, Verma, Ashutosh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143842/
https://www.ncbi.nlm.nih.gov/pubmed/25105546
http://dx.doi.org/10.3390/ijerph110807953
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author Rawlins, Julienne
Portanova, Alexandra
Zuckerman, Ilana
Loftis, Amanda
Ceccato, Pietro
Willingham, Arve Lee
Verma, Ashutosh
author_facet Rawlins, Julienne
Portanova, Alexandra
Zuckerman, Ilana
Loftis, Amanda
Ceccato, Pietro
Willingham, Arve Lee
Verma, Ashutosh
author_sort Rawlins, Julienne
collection PubMed
description Leptospirosis is an important waterborne zoonotic disease caused by pathogenic Leptospira. The pathogen is maintained in a population due to chronic colonization and shedding from renal tubules of domestic and wild animals. Humans and other animals become infected when they come in contact with urine from infected animals, either directly or through urine-contaminated surface water. In this study, we screened environmental water on the island of St. Kitts by using a TaqMan based real time quantitative polymerase chain reaction (qPCR) targeting a pathogen specific leptospiral gene, lipl32. Our results indicate that around one-fifth of tested water sources have detectable leptospiral DNA.
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spelling pubmed-41438422014-08-26 Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts Rawlins, Julienne Portanova, Alexandra Zuckerman, Ilana Loftis, Amanda Ceccato, Pietro Willingham, Arve Lee Verma, Ashutosh Int J Environ Res Public Health Communication Leptospirosis is an important waterborne zoonotic disease caused by pathogenic Leptospira. The pathogen is maintained in a population due to chronic colonization and shedding from renal tubules of domestic and wild animals. Humans and other animals become infected when they come in contact with urine from infected animals, either directly or through urine-contaminated surface water. In this study, we screened environmental water on the island of St. Kitts by using a TaqMan based real time quantitative polymerase chain reaction (qPCR) targeting a pathogen specific leptospiral gene, lipl32. Our results indicate that around one-fifth of tested water sources have detectable leptospiral DNA. MDPI 2014-08-07 2014-08 /pmc/articles/PMC4143842/ /pubmed/25105546 http://dx.doi.org/10.3390/ijerph110807953 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Communication
Rawlins, Julienne
Portanova, Alexandra
Zuckerman, Ilana
Loftis, Amanda
Ceccato, Pietro
Willingham, Arve Lee
Verma, Ashutosh
Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts
title Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts
title_full Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts
title_fullStr Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts
title_full_unstemmed Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts
title_short Molecular Detection of Leptospiral DNA in Environmental Water on St. Kitts
title_sort molecular detection of leptospiral dna in environmental water on st. kitts
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4143842/
https://www.ncbi.nlm.nih.gov/pubmed/25105546
http://dx.doi.org/10.3390/ijerph110807953
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