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Isolation and Characterisation of PRSV-P Resistance Genes in Carica and Vasconcellea

Papaya (Carica papaya L.) is one of the major tropical fruit crops worldwide, but it is limited throughout its range by papaya ringspot virus type P (PRSV-P). Previous genetic studies identified a functional PRSV-P resistance marker in a mapping population of F(2) plants of Vasconcellea pubescens (r...

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Autores principales: Razean Haireen, M. R., Drew, R. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4144303/
https://www.ncbi.nlm.nih.gov/pubmed/25184131
http://dx.doi.org/10.1155/2014/145403
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author Razean Haireen, M. R.
Drew, R. A.
author_facet Razean Haireen, M. R.
Drew, R. A.
author_sort Razean Haireen, M. R.
collection PubMed
description Papaya (Carica papaya L.) is one of the major tropical fruit crops worldwide, but it is limited throughout its range by papaya ringspot virus type P (PRSV-P). Previous genetic studies identified a functional PRSV-P resistance marker in a mapping population of F(2) plants of Vasconcellea pubescens (resistant to PRSV-P) × Vasconcellea parviflora (susceptible to PRSV-P) and showed that the marker exhibited homology to a serine threonine protein kinase (STK) gene. Full length cDNAs of putative PRSV-P resistance genes designated CP_STK from C. papaya and VP_STK1 and VP_STK2 from V. pubescens were cloned by rapid amplification of cDNA ends (RACE). Due to a frame-shift mutation, the two homologous sequences are transcribed and edited differently such that the gene product in V. pubescens is two separate transcripts, whereas in C. papaya they are fused into a single message. A peroxisomal targeting signal (PTS2) present in VP_STK2 but absent in the other transcripts may be the functional source of PRSV resistance in V. pubescens. The STK gene from V. pubescens may have been derived from an alternative splicing to confer resistance. The putative resistance gene, VP_STK2, that was identified in this study is a potential new source of PRSV-P resistance for papaya genotypes.
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spelling pubmed-41443032014-09-02 Isolation and Characterisation of PRSV-P Resistance Genes in Carica and Vasconcellea Razean Haireen, M. R. Drew, R. A. Int J Genomics Research Article Papaya (Carica papaya L.) is one of the major tropical fruit crops worldwide, but it is limited throughout its range by papaya ringspot virus type P (PRSV-P). Previous genetic studies identified a functional PRSV-P resistance marker in a mapping population of F(2) plants of Vasconcellea pubescens (resistant to PRSV-P) × Vasconcellea parviflora (susceptible to PRSV-P) and showed that the marker exhibited homology to a serine threonine protein kinase (STK) gene. Full length cDNAs of putative PRSV-P resistance genes designated CP_STK from C. papaya and VP_STK1 and VP_STK2 from V. pubescens were cloned by rapid amplification of cDNA ends (RACE). Due to a frame-shift mutation, the two homologous sequences are transcribed and edited differently such that the gene product in V. pubescens is two separate transcripts, whereas in C. papaya they are fused into a single message. A peroxisomal targeting signal (PTS2) present in VP_STK2 but absent in the other transcripts may be the functional source of PRSV resistance in V. pubescens. The STK gene from V. pubescens may have been derived from an alternative splicing to confer resistance. The putative resistance gene, VP_STK2, that was identified in this study is a potential new source of PRSV-P resistance for papaya genotypes. Hindawi Publishing Corporation 2014 2014-08-11 /pmc/articles/PMC4144303/ /pubmed/25184131 http://dx.doi.org/10.1155/2014/145403 Text en Copyright © 2014 M. R. Razean Haireen and R. A. Drew. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Razean Haireen, M. R.
Drew, R. A.
Isolation and Characterisation of PRSV-P Resistance Genes in Carica and Vasconcellea
title Isolation and Characterisation of PRSV-P Resistance Genes in Carica and Vasconcellea
title_full Isolation and Characterisation of PRSV-P Resistance Genes in Carica and Vasconcellea
title_fullStr Isolation and Characterisation of PRSV-P Resistance Genes in Carica and Vasconcellea
title_full_unstemmed Isolation and Characterisation of PRSV-P Resistance Genes in Carica and Vasconcellea
title_short Isolation and Characterisation of PRSV-P Resistance Genes in Carica and Vasconcellea
title_sort isolation and characterisation of prsv-p resistance genes in carica and vasconcellea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4144303/
https://www.ncbi.nlm.nih.gov/pubmed/25184131
http://dx.doi.org/10.1155/2014/145403
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