A Novel CXCR4-Selective High-Affinity Fluorescent Probe and Its Application in Competitive Binding Assays

[Image: see text] We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, wi...

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Autores principales: Yang, Yilei, Zhang, Qinghao, Gao, Mei, Yang, Xiaohong, Huang, Ziwei, An, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2014
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4144712/
https://www.ncbi.nlm.nih.gov/pubmed/25058910
http://dx.doi.org/10.1021/bi500500h
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author Yang, Yilei
Zhang, Qinghao
Gao, Mei
Yang, Xiaohong
Huang, Ziwei
An, Jing
author_facet Yang, Yilei
Zhang, Qinghao
Gao, Mei
Yang, Xiaohong
Huang, Ziwei
An, Jing
author_sort Yang, Yilei
collection PubMed
description [Image: see text] We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that seen with a conventional CXCR4 antibody. This nonradioactive, direct, and CXCR4-specific high-affinity screening system provides a new platform for CXCR4-targeted drug screening, as well as for the development of new probes for other GPCRs.
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spelling pubmed-41447122015-07-24 A Novel CXCR4-Selective High-Affinity Fluorescent Probe and Its Application in Competitive Binding Assays Yang, Yilei Zhang, Qinghao Gao, Mei Yang, Xiaohong Huang, Ziwei An, Jing Biochemistry [Image: see text] We recently developed a new, rapid, and specific bioassay system that employs a fluorescent probe fabricated from our discovered CXCR4-specific ligand DV1. This new probe sensitively and selectively blocks the binding of native and synthetic ligands to CXCR4 at nanomolar levels, with a capability comparable to that seen with a conventional CXCR4 antibody. This nonradioactive, direct, and CXCR4-specific high-affinity screening system provides a new platform for CXCR4-targeted drug screening, as well as for the development of new probes for other GPCRs. American Chemical Society 2014-07-24 2014-08-05 /pmc/articles/PMC4144712/ /pubmed/25058910 http://dx.doi.org/10.1021/bi500500h Text en Copyright © 2014 American Chemical Society Terms of Use (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html)
spellingShingle Yang, Yilei
Zhang, Qinghao
Gao, Mei
Yang, Xiaohong
Huang, Ziwei
An, Jing
A Novel CXCR4-Selective High-Affinity Fluorescent Probe and Its Application in Competitive Binding Assays
title A Novel CXCR4-Selective High-Affinity Fluorescent Probe and Its Application in Competitive Binding Assays
title_full A Novel CXCR4-Selective High-Affinity Fluorescent Probe and Its Application in Competitive Binding Assays
title_fullStr A Novel CXCR4-Selective High-Affinity Fluorescent Probe and Its Application in Competitive Binding Assays
title_full_unstemmed A Novel CXCR4-Selective High-Affinity Fluorescent Probe and Its Application in Competitive Binding Assays
title_short A Novel CXCR4-Selective High-Affinity Fluorescent Probe and Its Application in Competitive Binding Assays
title_sort novel cxcr4-selective high-affinity fluorescent probe and its application in competitive binding assays
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4144712/
https://www.ncbi.nlm.nih.gov/pubmed/25058910
http://dx.doi.org/10.1021/bi500500h
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