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Detecting Kinase Activities from Single Cell Lysate Using Concentration-Enhanced Mobility Shift Assay

[Image: see text] Electrokinetic preconcentration coupled with mobility shift assays can give rise to very high detection sensitivities. We describe a microfluidic device that utilizes this principle to detect cellular kinase activities by simultaneously concentrating and separating substrate peptid...

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Detalles Bibliográficos
Autores principales:	Cheow, Lih Feng, Sarkar, Aniruddh, Kolitz, Sarah, Lauffenburger, Douglas, Han, Jongyoon
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2014
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4144746/ https://www.ncbi.nlm.nih.gov/pubmed/25025773 http://dx.doi.org/10.1021/ac502185v

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