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Vascular endothelial growth factor genetic polymorphisms and susceptibility to age-related macular degeneration in Tunisian population
PURPOSE: Three VEGF SNPs (−2578) C/A, (+405) G/C and (+936) C/T were investigated in Tunisian exudative AMD patients in order to determine their association with the disease susceptibility and their influence to intravitreal bevacizumab therapy response. METHODS: 145 AMD patients and 207 age-matched...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145361/ https://www.ncbi.nlm.nih.gov/pubmed/25165559 http://dx.doi.org/10.1186/2050-7771-2-15 |
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author | Habibi, Imen Sfar, Imen Chebil, Ahmed Kort, Fedra Bouraoui, Rim Jendoubi-Ayed, Salwa Makhlouf, Mouna Abdallah, Taïeb Ben El Matri, Leila Gorgi, Yousr |
author_facet | Habibi, Imen Sfar, Imen Chebil, Ahmed Kort, Fedra Bouraoui, Rim Jendoubi-Ayed, Salwa Makhlouf, Mouna Abdallah, Taïeb Ben El Matri, Leila Gorgi, Yousr |
author_sort | Habibi, Imen |
collection | PubMed |
description | PURPOSE: Three VEGF SNPs (−2578) C/A, (+405) G/C and (+936) C/T were investigated in Tunisian exudative AMD patients in order to determine their association with the disease susceptibility and their influence to intravitreal bevacizumab therapy response. METHODS: 145 AMD patients and 207 age-matched controls were included. 68 patients were treated with intravitreal bevacizumab. SNPs genotyping were performed using direct sequencing. The serum VEGF was assayed by ELISA (R&D). RESULTS: The (+405) CC and (+936) TT genotypes were higher in AMD patients than in controls (p = 5 × 10(−6) and p = 0.021, respectively). The mean plasma levels of VEGF were statistically higher in AMD patients (84.22 pg/ml) than in controls (15 pg/ml). Three months after bevacizumab treatment, 52 patients (85.6%) were classified as good responders (GR) and 16 (14.4%) as poor responders (PR). The mean plasmatic-VEGF levels in GR patients was higher (86.61 ± 80.30 pg/ml) than in PR patients (47.12 ± 45.74 pg/ml) (p = 0.086). The patients with genotype homozygous TT (+936) would be PR compared to those carrying CT and CC genotypes. Whereas, those with AA (−2578) genotype would be GR compared with others genotypes (p = 0.014; p = 0.042 respectively). CONCLUSIONS: Our results show that VEGF genetic variants may contribute to the susceptibility to neovascular AMD in Tunisian patients. |
format | Online Article Text |
id | pubmed-4145361 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-41453612014-08-28 Vascular endothelial growth factor genetic polymorphisms and susceptibility to age-related macular degeneration in Tunisian population Habibi, Imen Sfar, Imen Chebil, Ahmed Kort, Fedra Bouraoui, Rim Jendoubi-Ayed, Salwa Makhlouf, Mouna Abdallah, Taïeb Ben El Matri, Leila Gorgi, Yousr Biomark Res Research PURPOSE: Three VEGF SNPs (−2578) C/A, (+405) G/C and (+936) C/T were investigated in Tunisian exudative AMD patients in order to determine their association with the disease susceptibility and their influence to intravitreal bevacizumab therapy response. METHODS: 145 AMD patients and 207 age-matched controls were included. 68 patients were treated with intravitreal bevacizumab. SNPs genotyping were performed using direct sequencing. The serum VEGF was assayed by ELISA (R&D). RESULTS: The (+405) CC and (+936) TT genotypes were higher in AMD patients than in controls (p = 5 × 10(−6) and p = 0.021, respectively). The mean plasma levels of VEGF were statistically higher in AMD patients (84.22 pg/ml) than in controls (15 pg/ml). Three months after bevacizumab treatment, 52 patients (85.6%) were classified as good responders (GR) and 16 (14.4%) as poor responders (PR). The mean plasmatic-VEGF levels in GR patients was higher (86.61 ± 80.30 pg/ml) than in PR patients (47.12 ± 45.74 pg/ml) (p = 0.086). The patients with genotype homozygous TT (+936) would be PR compared to those carrying CT and CC genotypes. Whereas, those with AA (−2578) genotype would be GR compared with others genotypes (p = 0.014; p = 0.042 respectively). CONCLUSIONS: Our results show that VEGF genetic variants may contribute to the susceptibility to neovascular AMD in Tunisian patients. BioMed Central 2014-08-18 /pmc/articles/PMC4145361/ /pubmed/25165559 http://dx.doi.org/10.1186/2050-7771-2-15 Text en Copyright © 2014 Habibi et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Habibi, Imen Sfar, Imen Chebil, Ahmed Kort, Fedra Bouraoui, Rim Jendoubi-Ayed, Salwa Makhlouf, Mouna Abdallah, Taïeb Ben El Matri, Leila Gorgi, Yousr Vascular endothelial growth factor genetic polymorphisms and susceptibility to age-related macular degeneration in Tunisian population |
title | Vascular endothelial growth factor genetic polymorphisms and susceptibility to age-related macular degeneration in Tunisian population |
title_full | Vascular endothelial growth factor genetic polymorphisms and susceptibility to age-related macular degeneration in Tunisian population |
title_fullStr | Vascular endothelial growth factor genetic polymorphisms and susceptibility to age-related macular degeneration in Tunisian population |
title_full_unstemmed | Vascular endothelial growth factor genetic polymorphisms and susceptibility to age-related macular degeneration in Tunisian population |
title_short | Vascular endothelial growth factor genetic polymorphisms and susceptibility to age-related macular degeneration in Tunisian population |
title_sort | vascular endothelial growth factor genetic polymorphisms and susceptibility to age-related macular degeneration in tunisian population |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145361/ https://www.ncbi.nlm.nih.gov/pubmed/25165559 http://dx.doi.org/10.1186/2050-7771-2-15 |
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