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A long non-coding RNA is required for targeting centromeric protein A to the human centromere

The centromere is a specialized chromatin region marked by the histone H3 variant CENP-A. Although active centromeric transcription has been documented for over a decade, the role of centromeric transcription or transcripts has been elusive. Here, we report that centromeric α-satellite transcription...

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Detalles Bibliográficos
Autores principales: Quénet, Delphine, Dalal, Yamini
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145801/
https://www.ncbi.nlm.nih.gov/pubmed/25117489
http://dx.doi.org/10.7554/eLife.03254
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author Quénet, Delphine
Dalal, Yamini
author_facet Quénet, Delphine
Dalal, Yamini
author_sort Quénet, Delphine
collection PubMed
description The centromere is a specialized chromatin region marked by the histone H3 variant CENP-A. Although active centromeric transcription has been documented for over a decade, the role of centromeric transcription or transcripts has been elusive. Here, we report that centromeric α-satellite transcription is dependent on RNA Polymerase II and occurs at late mitosis into early G1, concurrent with the timing of new CENP-A assembly. Inhibition of RNA Polymerase II-dependent transcription abrogates the recruitment of CENP-A and its chaperone HJURP to native human centromeres. Biochemical characterization of CENP-A associated RNAs reveals a 1.3 kb molecule that originates from centromeres, which physically interacts with the soluble pre-assembly HJURP/CENP-A complex in vivo, and whose down-regulation leads to the loss of CENP-A and HJURP at centromeres. This study describes a novel function for human centromeric long non-coding RNAs in the recruitment of HJURP and CENP-A, implicating RNA-based chaperone targeting in histone variant assembly.
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spelling pubmed-41458012014-10-17 A long non-coding RNA is required for targeting centromeric protein A to the human centromere Quénet, Delphine Dalal, Yamini eLife Neuroscience The centromere is a specialized chromatin region marked by the histone H3 variant CENP-A. Although active centromeric transcription has been documented for over a decade, the role of centromeric transcription or transcripts has been elusive. Here, we report that centromeric α-satellite transcription is dependent on RNA Polymerase II and occurs at late mitosis into early G1, concurrent with the timing of new CENP-A assembly. Inhibition of RNA Polymerase II-dependent transcription abrogates the recruitment of CENP-A and its chaperone HJURP to native human centromeres. Biochemical characterization of CENP-A associated RNAs reveals a 1.3 kb molecule that originates from centromeres, which physically interacts with the soluble pre-assembly HJURP/CENP-A complex in vivo, and whose down-regulation leads to the loss of CENP-A and HJURP at centromeres. This study describes a novel function for human centromeric long non-coding RNAs in the recruitment of HJURP and CENP-A, implicating RNA-based chaperone targeting in histone variant assembly. eLife Sciences Publications, Ltd 2014-08-12 /pmc/articles/PMC4145801/ /pubmed/25117489 http://dx.doi.org/10.7554/eLife.03254 Text en © 2014, Quénet et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Quénet, Delphine
Dalal, Yamini
A long non-coding RNA is required for targeting centromeric protein A to the human centromere
title A long non-coding RNA is required for targeting centromeric protein A to the human centromere
title_full A long non-coding RNA is required for targeting centromeric protein A to the human centromere
title_fullStr A long non-coding RNA is required for targeting centromeric protein A to the human centromere
title_full_unstemmed A long non-coding RNA is required for targeting centromeric protein A to the human centromere
title_short A long non-coding RNA is required for targeting centromeric protein A to the human centromere
title_sort long non-coding rna is required for targeting centromeric protein a to the human centromere
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145801/
https://www.ncbi.nlm.nih.gov/pubmed/25117489
http://dx.doi.org/10.7554/eLife.03254
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