Cargando…
A long non-coding RNA is required for targeting centromeric protein A to the human centromere
The centromere is a specialized chromatin region marked by the histone H3 variant CENP-A. Although active centromeric transcription has been documented for over a decade, the role of centromeric transcription or transcripts has been elusive. Here, we report that centromeric α-satellite transcription...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145801/ https://www.ncbi.nlm.nih.gov/pubmed/25117489 http://dx.doi.org/10.7554/eLife.03254 |
_version_ | 1782332224250576896 |
---|---|
author | Quénet, Delphine Dalal, Yamini |
author_facet | Quénet, Delphine Dalal, Yamini |
author_sort | Quénet, Delphine |
collection | PubMed |
description | The centromere is a specialized chromatin region marked by the histone H3 variant CENP-A. Although active centromeric transcription has been documented for over a decade, the role of centromeric transcription or transcripts has been elusive. Here, we report that centromeric α-satellite transcription is dependent on RNA Polymerase II and occurs at late mitosis into early G1, concurrent with the timing of new CENP-A assembly. Inhibition of RNA Polymerase II-dependent transcription abrogates the recruitment of CENP-A and its chaperone HJURP to native human centromeres. Biochemical characterization of CENP-A associated RNAs reveals a 1.3 kb molecule that originates from centromeres, which physically interacts with the soluble pre-assembly HJURP/CENP-A complex in vivo, and whose down-regulation leads to the loss of CENP-A and HJURP at centromeres. This study describes a novel function for human centromeric long non-coding RNAs in the recruitment of HJURP and CENP-A, implicating RNA-based chaperone targeting in histone variant assembly. |
format | Online Article Text |
id | pubmed-4145801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-41458012014-10-17 A long non-coding RNA is required for targeting centromeric protein A to the human centromere Quénet, Delphine Dalal, Yamini eLife Neuroscience The centromere is a specialized chromatin region marked by the histone H3 variant CENP-A. Although active centromeric transcription has been documented for over a decade, the role of centromeric transcription or transcripts has been elusive. Here, we report that centromeric α-satellite transcription is dependent on RNA Polymerase II and occurs at late mitosis into early G1, concurrent with the timing of new CENP-A assembly. Inhibition of RNA Polymerase II-dependent transcription abrogates the recruitment of CENP-A and its chaperone HJURP to native human centromeres. Biochemical characterization of CENP-A associated RNAs reveals a 1.3 kb molecule that originates from centromeres, which physically interacts with the soluble pre-assembly HJURP/CENP-A complex in vivo, and whose down-regulation leads to the loss of CENP-A and HJURP at centromeres. This study describes a novel function for human centromeric long non-coding RNAs in the recruitment of HJURP and CENP-A, implicating RNA-based chaperone targeting in histone variant assembly. eLife Sciences Publications, Ltd 2014-08-12 /pmc/articles/PMC4145801/ /pubmed/25117489 http://dx.doi.org/10.7554/eLife.03254 Text en © 2014, Quénet et al http://creativecommons.org/licenses/by/4.0/ http://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Neuroscience Quénet, Delphine Dalal, Yamini A long non-coding RNA is required for targeting centromeric protein A to the human centromere |
title | A long non-coding RNA is required for targeting centromeric protein A to the human centromere |
title_full | A long non-coding RNA is required for targeting centromeric protein A to the human centromere |
title_fullStr | A long non-coding RNA is required for targeting centromeric protein A to the human centromere |
title_full_unstemmed | A long non-coding RNA is required for targeting centromeric protein A to the human centromere |
title_short | A long non-coding RNA is required for targeting centromeric protein A to the human centromere |
title_sort | long non-coding rna is required for targeting centromeric protein a to the human centromere |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145801/ https://www.ncbi.nlm.nih.gov/pubmed/25117489 http://dx.doi.org/10.7554/eLife.03254 |
work_keys_str_mv | AT quenetdelphine alongnoncodingrnaisrequiredfortargetingcentromericproteinatothehumancentromere AT dalalyamini alongnoncodingrnaisrequiredfortargetingcentromericproteinatothehumancentromere AT quenetdelphine longnoncodingrnaisrequiredfortargetingcentromericproteinatothehumancentromere AT dalalyamini longnoncodingrnaisrequiredfortargetingcentromericproteinatothehumancentromere |