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Morphological and physiological species-dependent characteristics of the rodent Grueneberg ganglion

In the mouse, the Grueneberg ganglion (GG) is an olfactory subsystem implicated both in chemo- and thermo-sensing. It is specifically involved in the recognition of volatile danger cues such as alarm pheromones and structurally-related predator scents. No evidence for these GG sensory functions has...

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Autores principales: Brechbühl, Julien, Klaey, Magali, Moine, Fabian, Bovay, Esther, Hurni, Nicolas, Nenniger-Tosato, Monique, Broillet, Marie-Christine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145810/
https://www.ncbi.nlm.nih.gov/pubmed/25221478
http://dx.doi.org/10.3389/fnana.2014.00087
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author Brechbühl, Julien
Klaey, Magali
Moine, Fabian
Bovay, Esther
Hurni, Nicolas
Nenniger-Tosato, Monique
Broillet, Marie-Christine
author_facet Brechbühl, Julien
Klaey, Magali
Moine, Fabian
Bovay, Esther
Hurni, Nicolas
Nenniger-Tosato, Monique
Broillet, Marie-Christine
author_sort Brechbühl, Julien
collection PubMed
description In the mouse, the Grueneberg ganglion (GG) is an olfactory subsystem implicated both in chemo- and thermo-sensing. It is specifically involved in the recognition of volatile danger cues such as alarm pheromones and structurally-related predator scents. No evidence for these GG sensory functions has been reported yet in other rodent species. In this study, we used a combination of histological and physiological techniques to verify the presence of a GG and investigate its function in the rat, hamster, and gerbil comparing with the mouse. By scanning electron microscopy (SEM) and transmitted electron microscopy (TEM), we found isolated or groups of large GG cells of different shapes that in spite of their gross anatomical similarities, display important structural differences between species. We performed a comparative and morphological study focusing on the conserved olfactory features of these cells. We found fine ciliary processes, mostly wrapped in ensheating glial cells, in variable number of clusters deeply invaginated in the neuronal soma. Interestingly, the glial wrapping, the amount of microtubules and their distribution in the ciliary processes were different between rodents. Using immunohistochemistry, we were able to detect the expression of known GG proteins, such as the membrane guanylyl cyclase G and the cyclic nucleotide-gated channel A3. Both the expression and the subcellular localization of these signaling proteins were found to be species-dependent. Calcium imaging experiments on acute tissue slice preparations from rodent GG demonstrated that the chemo- and thermo-evoked neuronal responses were different between species. Thus, GG neurons from mice and rats displayed both chemo- and thermo-sensing, while hamsters and gerbils showed profound differences in their sensitivities. We suggest that the integrative comparison between the structural morphologies, the sensory properties, and the ethological contexts supports species-dependent GG features prompted by the environmental pressure.
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spelling pubmed-41458102014-09-12 Morphological and physiological species-dependent characteristics of the rodent Grueneberg ganglion Brechbühl, Julien Klaey, Magali Moine, Fabian Bovay, Esther Hurni, Nicolas Nenniger-Tosato, Monique Broillet, Marie-Christine Front Neuroanat Neuroscience In the mouse, the Grueneberg ganglion (GG) is an olfactory subsystem implicated both in chemo- and thermo-sensing. It is specifically involved in the recognition of volatile danger cues such as alarm pheromones and structurally-related predator scents. No evidence for these GG sensory functions has been reported yet in other rodent species. In this study, we used a combination of histological and physiological techniques to verify the presence of a GG and investigate its function in the rat, hamster, and gerbil comparing with the mouse. By scanning electron microscopy (SEM) and transmitted electron microscopy (TEM), we found isolated or groups of large GG cells of different shapes that in spite of their gross anatomical similarities, display important structural differences between species. We performed a comparative and morphological study focusing on the conserved olfactory features of these cells. We found fine ciliary processes, mostly wrapped in ensheating glial cells, in variable number of clusters deeply invaginated in the neuronal soma. Interestingly, the glial wrapping, the amount of microtubules and their distribution in the ciliary processes were different between rodents. Using immunohistochemistry, we were able to detect the expression of known GG proteins, such as the membrane guanylyl cyclase G and the cyclic nucleotide-gated channel A3. Both the expression and the subcellular localization of these signaling proteins were found to be species-dependent. Calcium imaging experiments on acute tissue slice preparations from rodent GG demonstrated that the chemo- and thermo-evoked neuronal responses were different between species. Thus, GG neurons from mice and rats displayed both chemo- and thermo-sensing, while hamsters and gerbils showed profound differences in their sensitivities. We suggest that the integrative comparison between the structural morphologies, the sensory properties, and the ethological contexts supports species-dependent GG features prompted by the environmental pressure. Frontiers Media S.A. 2014-08-27 /pmc/articles/PMC4145810/ /pubmed/25221478 http://dx.doi.org/10.3389/fnana.2014.00087 Text en Copyright © 2014 Brechbühl, Klaey, Moine, Bovay, Hurni, Nenniger-Tosato and Broillet. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Brechbühl, Julien
Klaey, Magali
Moine, Fabian
Bovay, Esther
Hurni, Nicolas
Nenniger-Tosato, Monique
Broillet, Marie-Christine
Morphological and physiological species-dependent characteristics of the rodent Grueneberg ganglion
title Morphological and physiological species-dependent characteristics of the rodent Grueneberg ganglion
title_full Morphological and physiological species-dependent characteristics of the rodent Grueneberg ganglion
title_fullStr Morphological and physiological species-dependent characteristics of the rodent Grueneberg ganglion
title_full_unstemmed Morphological and physiological species-dependent characteristics of the rodent Grueneberg ganglion
title_short Morphological and physiological species-dependent characteristics of the rodent Grueneberg ganglion
title_sort morphological and physiological species-dependent characteristics of the rodent grueneberg ganglion
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145810/
https://www.ncbi.nlm.nih.gov/pubmed/25221478
http://dx.doi.org/10.3389/fnana.2014.00087
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