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Local delivery of minocycline-loaded PEG-PLA nanoparticles for the enhanced treatment of periodontitis in dogs

BACKGROUND: Rapid local drug clearance of antimicrobials is a major drawback for the treatment of chronic periodontitis. In the study reported here, minocycline-loaded poly(ethylene glycol)-poly(lactic acid) nanoparticles were prepared and administered locally for long drug retention and enhanced tr...

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Autores principales: Yao, Wenxin, Xu, Peicheng, Pang, Zhiqing, Zhao, Jingjing, Chai, Zhilan, Li, Xiaoxia, Li, Huan, Jiang, Menglin, Cheng, Hongbo, Zhang, Bo, Cheng, Nengneng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145825/
https://www.ncbi.nlm.nih.gov/pubmed/25170266
http://dx.doi.org/10.2147/IJN.S67521
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author Yao, Wenxin
Xu, Peicheng
Pang, Zhiqing
Zhao, Jingjing
Chai, Zhilan
Li, Xiaoxia
Li, Huan
Jiang, Menglin
Cheng, Hongbo
Zhang, Bo
Cheng, Nengneng
author_facet Yao, Wenxin
Xu, Peicheng
Pang, Zhiqing
Zhao, Jingjing
Chai, Zhilan
Li, Xiaoxia
Li, Huan
Jiang, Menglin
Cheng, Hongbo
Zhang, Bo
Cheng, Nengneng
author_sort Yao, Wenxin
collection PubMed
description BACKGROUND: Rapid local drug clearance of antimicrobials is a major drawback for the treatment of chronic periodontitis. In the study reported here, minocycline-loaded poly(ethylene glycol)-poly(lactic acid) nanoparticles were prepared and administered locally for long drug retention and enhanced treatment of periodontitis in dogs. METHODS: Biodegradable poly(ethylene glycol)-poly(lactic acid) was synthesized to prepare nanoparticles using an emulsion/solvent evaporation technique. The particle size and zeta potential of the minocycline-loaded nanoparticles (MIN-NPs) were determined by dynamic light scattering and the morphology of the nanoparticles was observed by transmission electron microscopy. The in vitro release of minocycline from MIN-NPs and in vivo pharmacokinetics of minocycline in gingival crevice fluid, after local administration of MIN-NPs in the periodontal pockets of beagle dogs with periodontitis, were investigated. The anti-periodontitis effects of MIN-NPs on periodontitis-bearing dogs were finally evaluated. RESULTS: Transmission electron microscopy examination and dynamic light scattering results revealed that the MIN-NPs had a round shape, with a mean diameter around 100 nm. The in vitro release of minocycline from MIN-NPs showed a remarkably sustained releasing characteristic. After local administration of the MIN-NPs, minocycline concentration in gingival crevice fluid decreased slowly and retained an effective drug concentration for a longer time (12 days) than Periocline(®). Anti-periodontitis effects demonstrated that MIN-NPs could significantly decrease symptoms of periodontitis compared with Periocline and minocycline solution. These findings suggest that MIN-NPs might have great potential in the treatment of periodontitis.
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spelling pubmed-41458252014-08-28 Local delivery of minocycline-loaded PEG-PLA nanoparticles for the enhanced treatment of periodontitis in dogs Yao, Wenxin Xu, Peicheng Pang, Zhiqing Zhao, Jingjing Chai, Zhilan Li, Xiaoxia Li, Huan Jiang, Menglin Cheng, Hongbo Zhang, Bo Cheng, Nengneng Int J Nanomedicine Original Research BACKGROUND: Rapid local drug clearance of antimicrobials is a major drawback for the treatment of chronic periodontitis. In the study reported here, minocycline-loaded poly(ethylene glycol)-poly(lactic acid) nanoparticles were prepared and administered locally for long drug retention and enhanced treatment of periodontitis in dogs. METHODS: Biodegradable poly(ethylene glycol)-poly(lactic acid) was synthesized to prepare nanoparticles using an emulsion/solvent evaporation technique. The particle size and zeta potential of the minocycline-loaded nanoparticles (MIN-NPs) were determined by dynamic light scattering and the morphology of the nanoparticles was observed by transmission electron microscopy. The in vitro release of minocycline from MIN-NPs and in vivo pharmacokinetics of minocycline in gingival crevice fluid, after local administration of MIN-NPs in the periodontal pockets of beagle dogs with periodontitis, were investigated. The anti-periodontitis effects of MIN-NPs on periodontitis-bearing dogs were finally evaluated. RESULTS: Transmission electron microscopy examination and dynamic light scattering results revealed that the MIN-NPs had a round shape, with a mean diameter around 100 nm. The in vitro release of minocycline from MIN-NPs showed a remarkably sustained releasing characteristic. After local administration of the MIN-NPs, minocycline concentration in gingival crevice fluid decreased slowly and retained an effective drug concentration for a longer time (12 days) than Periocline(®). Anti-periodontitis effects demonstrated that MIN-NPs could significantly decrease symptoms of periodontitis compared with Periocline and minocycline solution. These findings suggest that MIN-NPs might have great potential in the treatment of periodontitis. Dove Medical Press 2014-08-18 /pmc/articles/PMC4145825/ /pubmed/25170266 http://dx.doi.org/10.2147/IJN.S67521 Text en © 2014 Yao et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Yao, Wenxin
Xu, Peicheng
Pang, Zhiqing
Zhao, Jingjing
Chai, Zhilan
Li, Xiaoxia
Li, Huan
Jiang, Menglin
Cheng, Hongbo
Zhang, Bo
Cheng, Nengneng
Local delivery of minocycline-loaded PEG-PLA nanoparticles for the enhanced treatment of periodontitis in dogs
title Local delivery of minocycline-loaded PEG-PLA nanoparticles for the enhanced treatment of periodontitis in dogs
title_full Local delivery of minocycline-loaded PEG-PLA nanoparticles for the enhanced treatment of periodontitis in dogs
title_fullStr Local delivery of minocycline-loaded PEG-PLA nanoparticles for the enhanced treatment of periodontitis in dogs
title_full_unstemmed Local delivery of minocycline-loaded PEG-PLA nanoparticles for the enhanced treatment of periodontitis in dogs
title_short Local delivery of minocycline-loaded PEG-PLA nanoparticles for the enhanced treatment of periodontitis in dogs
title_sort local delivery of minocycline-loaded peg-pla nanoparticles for the enhanced treatment of periodontitis in dogs
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145825/
https://www.ncbi.nlm.nih.gov/pubmed/25170266
http://dx.doi.org/10.2147/IJN.S67521
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