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Activin A prevents neuron-like PC12 cell apoptosis after oxygen-glucose deprivation☆
In this study, PC12 cells were induced to differentiate into neuron-like cells using nerve growth factor, and were subjected to oxygen-glucose deprivation. Cells were treated with 0, 10, 20, 30, 50, 100 ng/mL exogenous Activin A. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assa...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145885/ https://www.ncbi.nlm.nih.gov/pubmed/25206395 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.11.007 |
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author | Xu, Guihua He, Jinting Guo, Hongliang Mei, Chunli Wang, Jiaoqi Li, Zhongshu Chen, Han Mang, Jing Yang, Hong Xu, Zhongxin |
author_facet | Xu, Guihua He, Jinting Guo, Hongliang Mei, Chunli Wang, Jiaoqi Li, Zhongshu Chen, Han Mang, Jing Yang, Hong Xu, Zhongxin |
author_sort | Xu, Guihua |
collection | PubMed |
description | In this study, PC12 cells were induced to differentiate into neuron-like cells using nerve growth factor, and were subjected to oxygen-glucose deprivation. Cells were treated with 0, 10, 20, 30, 50, 100 ng/mL exogenous Activin A. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay and Hoechst 33324 staining showed that the survival percentage of PC12 cells significantly decreased and the rate of apoptosis significantly increased after oxygen-glucose deprivation. Exogenous Activin A significantly increased the survival percentage of PC12 cells in a dose-dependent manner. Reverse transcription-PCR results revealed a significant increase in Activin receptor IIA, Smad3 and Smad4 mRNA levels, which are key sites in the Activin A/Smads signaling pathway, in neuron-like cells subjected to oxygen-glucose deprivation, while mRNA expression of the apoptosis-regulation gene caspase-3 decreased. Our experimental findings indicate that exogenous Activin A plays an anti-apoptotic role and protects neurons by means of activating the Activin A/Smads signaling pathway. |
format | Online Article Text |
id | pubmed-4145885 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-41458852014-09-09 Activin A prevents neuron-like PC12 cell apoptosis after oxygen-glucose deprivation☆ Xu, Guihua He, Jinting Guo, Hongliang Mei, Chunli Wang, Jiaoqi Li, Zhongshu Chen, Han Mang, Jing Yang, Hong Xu, Zhongxin Neural Regen Res Neurodegenerative Disease and Neural Regeneration In this study, PC12 cells were induced to differentiate into neuron-like cells using nerve growth factor, and were subjected to oxygen-glucose deprivation. Cells were treated with 0, 10, 20, 30, 50, 100 ng/mL exogenous Activin A. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl tetrazolium bromide assay and Hoechst 33324 staining showed that the survival percentage of PC12 cells significantly decreased and the rate of apoptosis significantly increased after oxygen-glucose deprivation. Exogenous Activin A significantly increased the survival percentage of PC12 cells in a dose-dependent manner. Reverse transcription-PCR results revealed a significant increase in Activin receptor IIA, Smad3 and Smad4 mRNA levels, which are key sites in the Activin A/Smads signaling pathway, in neuron-like cells subjected to oxygen-glucose deprivation, while mRNA expression of the apoptosis-regulation gene caspase-3 decreased. Our experimental findings indicate that exogenous Activin A plays an anti-apoptotic role and protects neurons by means of activating the Activin A/Smads signaling pathway. Medknow Publications & Media Pvt Ltd 2013-04-15 /pmc/articles/PMC4145885/ /pubmed/25206395 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.11.007 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Neurodegenerative Disease and Neural Regeneration Xu, Guihua He, Jinting Guo, Hongliang Mei, Chunli Wang, Jiaoqi Li, Zhongshu Chen, Han Mang, Jing Yang, Hong Xu, Zhongxin Activin A prevents neuron-like PC12 cell apoptosis after oxygen-glucose deprivation☆ |
title | Activin A prevents neuron-like PC12 cell apoptosis after oxygen-glucose deprivation☆ |
title_full | Activin A prevents neuron-like PC12 cell apoptosis after oxygen-glucose deprivation☆ |
title_fullStr | Activin A prevents neuron-like PC12 cell apoptosis after oxygen-glucose deprivation☆ |
title_full_unstemmed | Activin A prevents neuron-like PC12 cell apoptosis after oxygen-glucose deprivation☆ |
title_short | Activin A prevents neuron-like PC12 cell apoptosis after oxygen-glucose deprivation☆ |
title_sort | activin a prevents neuron-like pc12 cell apoptosis after oxygen-glucose deprivation☆ |
topic | Neurodegenerative Disease and Neural Regeneration |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145885/ https://www.ncbi.nlm.nih.gov/pubmed/25206395 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.11.007 |
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