Cargando…
Ketamine induces tau hyperphosphorylation at serine 404 in the hippocampus of neonatal rats
Male Wistar 7-day-old rats were injected with 40 mg/kg ketamine intraperitoneally, followed by three additional injections of 20 mg/kg ketamine each upon restoration of the righting reflex. Neonatal rats injected with equivalent volumes of saline served as controls. Hippocampal samples were collecte...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145967/ https://www.ncbi.nlm.nih.gov/pubmed/25206455 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.17.007 |
_version_ | 1782332252119629824 |
---|---|
author | Jin, Haiyan Hu, Zhiyong Dong, Mengjie Wu, Yidong Zhu, Zhirui Xu, Lili |
author_facet | Jin, Haiyan Hu, Zhiyong Dong, Mengjie Wu, Yidong Zhu, Zhirui Xu, Lili |
author_sort | Jin, Haiyan |
collection | PubMed |
description | Male Wistar 7-day-old rats were injected with 40 mg/kg ketamine intraperitoneally, followed by three additional injections of 20 mg/kg ketamine each upon restoration of the righting reflex. Neonatal rats injected with equivalent volumes of saline served as controls. Hippocampal samples were collected at 1, 7 or 14 days following administration. Electron microscopy showed that neuronal structure changed noticeably following ketamine treatment. Specifically, microtubular structure became irregular and disorganized. Quantitative real time-PCR revealed that phosphorylated tau mRNA was upregulated after ketamine. Western blot analysis demonstrated that phosphorylated tau levels at serine 396 initially decreased at 1 day after ketamine injection, and then gradually returned to control values. At 14 days after injection, levels of phosphorylated tau were higher in the ketamine group than in the control group. Tau protein phosphorylated at serine 404 significantly increased after ketamine injection, and then gradually decreased with time. However, the levels of tau protein at serine 404 were significantly greater in the ketamine group than in the control group until 14 days. The present results indicate that ketamine induces an increase of phosphorylated tau mRNA and excessive phosphorylation of tau protein at serine 404, causing disruption of microtubules in the neonatal rat hippocampus and potentially resulting in damage to hippocampal neurons. |
format | Online Article Text |
id | pubmed-4145967 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-41459672014-09-09 Ketamine induces tau hyperphosphorylation at serine 404 in the hippocampus of neonatal rats Jin, Haiyan Hu, Zhiyong Dong, Mengjie Wu, Yidong Zhu, Zhirui Xu, Lili Neural Regen Res Neurodegenerative Disease and Neural Regeneration Male Wistar 7-day-old rats were injected with 40 mg/kg ketamine intraperitoneally, followed by three additional injections of 20 mg/kg ketamine each upon restoration of the righting reflex. Neonatal rats injected with equivalent volumes of saline served as controls. Hippocampal samples were collected at 1, 7 or 14 days following administration. Electron microscopy showed that neuronal structure changed noticeably following ketamine treatment. Specifically, microtubular structure became irregular and disorganized. Quantitative real time-PCR revealed that phosphorylated tau mRNA was upregulated after ketamine. Western blot analysis demonstrated that phosphorylated tau levels at serine 396 initially decreased at 1 day after ketamine injection, and then gradually returned to control values. At 14 days after injection, levels of phosphorylated tau were higher in the ketamine group than in the control group. Tau protein phosphorylated at serine 404 significantly increased after ketamine injection, and then gradually decreased with time. However, the levels of tau protein at serine 404 were significantly greater in the ketamine group than in the control group until 14 days. The present results indicate that ketamine induces an increase of phosphorylated tau mRNA and excessive phosphorylation of tau protein at serine 404, causing disruption of microtubules in the neonatal rat hippocampus and potentially resulting in damage to hippocampal neurons. Medknow Publications & Media Pvt Ltd 2013-06-15 /pmc/articles/PMC4145967/ /pubmed/25206455 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.17.007 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Neurodegenerative Disease and Neural Regeneration Jin, Haiyan Hu, Zhiyong Dong, Mengjie Wu, Yidong Zhu, Zhirui Xu, Lili Ketamine induces tau hyperphosphorylation at serine 404 in the hippocampus of neonatal rats |
title | Ketamine induces tau hyperphosphorylation at serine 404 in the hippocampus of neonatal rats |
title_full | Ketamine induces tau hyperphosphorylation at serine 404 in the hippocampus of neonatal rats |
title_fullStr | Ketamine induces tau hyperphosphorylation at serine 404 in the hippocampus of neonatal rats |
title_full_unstemmed | Ketamine induces tau hyperphosphorylation at serine 404 in the hippocampus of neonatal rats |
title_short | Ketamine induces tau hyperphosphorylation at serine 404 in the hippocampus of neonatal rats |
title_sort | ketamine induces tau hyperphosphorylation at serine 404 in the hippocampus of neonatal rats |
topic | Neurodegenerative Disease and Neural Regeneration |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4145967/ https://www.ncbi.nlm.nih.gov/pubmed/25206455 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.17.007 |
work_keys_str_mv | AT jinhaiyan ketamineinducestauhyperphosphorylationatserine404inthehippocampusofneonatalrats AT huzhiyong ketamineinducestauhyperphosphorylationatserine404inthehippocampusofneonatalrats AT dongmengjie ketamineinducestauhyperphosphorylationatserine404inthehippocampusofneonatalrats AT wuyidong ketamineinducestauhyperphosphorylationatserine404inthehippocampusofneonatalrats AT zhuzhirui ketamineinducestauhyperphosphorylationatserine404inthehippocampusofneonatalrats AT xulili ketamineinducestauhyperphosphorylationatserine404inthehippocampusofneonatalrats |