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Rat hair follicle stem cells differentiate and promote recovery following spinal cord injury

Emerging studies of treating spinal cord injury (SCI) with adult stem cells led us to evaluate the effects of transplantation of hair follicle stem cells in rats with a compression-induced spinal cord lesion. Here, we proposed a hypothesis that rat hair follicle stem cell transplantation can promote...

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Autores principales: Najafzadeh, Nowruz, Nobakht, Maliheh, Pourheydar, Bagher, Golmohammadi, Mohammad Ghasem
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146002/
https://www.ncbi.nlm.nih.gov/pubmed/25206658
http://dx.doi.org/10.3969/j.issn.1673-5374.2013.36.001
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author Najafzadeh, Nowruz
Nobakht, Maliheh
Pourheydar, Bagher
Golmohammadi, Mohammad Ghasem
author_facet Najafzadeh, Nowruz
Nobakht, Maliheh
Pourheydar, Bagher
Golmohammadi, Mohammad Ghasem
author_sort Najafzadeh, Nowruz
collection PubMed
description Emerging studies of treating spinal cord injury (SCI) with adult stem cells led us to evaluate the effects of transplantation of hair follicle stem cells in rats with a compression-induced spinal cord lesion. Here, we proposed a hypothesis that rat hair follicle stem cell transplantation can promote the recovery of injured spinal cord. Compression-induced spinal cord injury was induced in Wistar rats in this study. The bulge area of the rat vibrissa follicles was isolated, cultivated and characterized with nestin as a stem cell marker. 5-Bromo-2′-deoxyuridine (BrdU) labeled bulge stem cells were transplanted into rats with spinal cord injury. Immunohistochemical staining results showed that some of the grafted cells could survive and differentiate into oligodendrocytes (receptor-interacting protein positive cells) and neuronal-like cells (βIII-tubulin positive cells) at 3 weeks after transplantation. In addition, recovery of hind limb locomotor function in spinal cord injury rats at 8 weeks following cell transplantation was assessed using the Basso, Beattie and Bresnahan (BBB) locomotor rating scale. The results demonstrate that the grafted hair follicle stem cells can survive for a long time period in vivo and differentiate into neuronal- and glial-like cells. These results suggest that hair follicle stem cells can promote the recovery of spinal cord injury.
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spelling pubmed-41460022014-09-09 Rat hair follicle stem cells differentiate and promote recovery following spinal cord injury Najafzadeh, Nowruz Nobakht, Maliheh Pourheydar, Bagher Golmohammadi, Mohammad Ghasem Neural Regen Res Research and Report Article: Spinal Cord Injury and Neural Regeneration Emerging studies of treating spinal cord injury (SCI) with adult stem cells led us to evaluate the effects of transplantation of hair follicle stem cells in rats with a compression-induced spinal cord lesion. Here, we proposed a hypothesis that rat hair follicle stem cell transplantation can promote the recovery of injured spinal cord. Compression-induced spinal cord injury was induced in Wistar rats in this study. The bulge area of the rat vibrissa follicles was isolated, cultivated and characterized with nestin as a stem cell marker. 5-Bromo-2′-deoxyuridine (BrdU) labeled bulge stem cells were transplanted into rats with spinal cord injury. Immunohistochemical staining results showed that some of the grafted cells could survive and differentiate into oligodendrocytes (receptor-interacting protein positive cells) and neuronal-like cells (βIII-tubulin positive cells) at 3 weeks after transplantation. In addition, recovery of hind limb locomotor function in spinal cord injury rats at 8 weeks following cell transplantation was assessed using the Basso, Beattie and Bresnahan (BBB) locomotor rating scale. The results demonstrate that the grafted hair follicle stem cells can survive for a long time period in vivo and differentiate into neuronal- and glial-like cells. These results suggest that hair follicle stem cells can promote the recovery of spinal cord injury. Medknow Publications & Media Pvt Ltd 2013-12-25 /pmc/articles/PMC4146002/ /pubmed/25206658 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.36.001 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report Article: Spinal Cord Injury and Neural Regeneration
Najafzadeh, Nowruz
Nobakht, Maliheh
Pourheydar, Bagher
Golmohammadi, Mohammad Ghasem
Rat hair follicle stem cells differentiate and promote recovery following spinal cord injury
title Rat hair follicle stem cells differentiate and promote recovery following spinal cord injury
title_full Rat hair follicle stem cells differentiate and promote recovery following spinal cord injury
title_fullStr Rat hair follicle stem cells differentiate and promote recovery following spinal cord injury
title_full_unstemmed Rat hair follicle stem cells differentiate and promote recovery following spinal cord injury
title_short Rat hair follicle stem cells differentiate and promote recovery following spinal cord injury
title_sort rat hair follicle stem cells differentiate and promote recovery following spinal cord injury
topic Research and Report Article: Spinal Cord Injury and Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146002/
https://www.ncbi.nlm.nih.gov/pubmed/25206658
http://dx.doi.org/10.3969/j.issn.1673-5374.2013.36.001
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