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Hypothyroidism affects astrocyte and microglial morphology in type 2 diabetes

In the present study, we investigated the effects of hypothyroidism on the morphology of astrocytes and microglia in the hippocampus of Zucker diabetic fatty rats and Zucker lean control rats. To induce hypothyroidism, Zucker lean control and Zucker diabetic fatty rats at 7 weeks of age orally recei...

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Autores principales: Nam, Sung Min, Kim, Yo Na, Yoo, Dae Young, Yi, Sun Shin, Choi, Jung Hoon, Hwang, In Koo, Seong, Je Kyung, Yoon, Yeo Sung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146114/
https://www.ncbi.nlm.nih.gov/pubmed/25206556
http://dx.doi.org/10.3969/j.issn.1673-5374.2013.26.007
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author Nam, Sung Min
Kim, Yo Na
Yoo, Dae Young
Yi, Sun Shin
Choi, Jung Hoon
Hwang, In Koo
Seong, Je Kyung
Yoon, Yeo Sung
author_facet Nam, Sung Min
Kim, Yo Na
Yoo, Dae Young
Yi, Sun Shin
Choi, Jung Hoon
Hwang, In Koo
Seong, Je Kyung
Yoon, Yeo Sung
author_sort Nam, Sung Min
collection PubMed
description In the present study, we investigated the effects of hypothyroidism on the morphology of astrocytes and microglia in the hippocampus of Zucker diabetic fatty rats and Zucker lean control rats. To induce hypothyroidism, Zucker lean control and Zucker diabetic fatty rats at 7 weeks of age orally received the vehicle or methimazole, an anti-thyroid drug, treatment for 5 weeks and were sacrificed at 12 weeks of age in all groups for blood chemistry and immunohistochemical staining. In the methimazole-treated Zucker lean control and Zucker diabetic fatty rats, the serum circulating thyronine (T(3)) and thyroxine (T(4)) levels were significantly decreased compared to levels observed in the vehicle-treated Zucker lean control or Zucker diabetic fatty rats. This reduction was more prominent in the methimazole-treated Zucker diabetic fatty group. Glial fibrillary acidic protein immunoreactive astrocytes and ionized calcium-binding adapter molecule 1 (Iba-1)-immunoreactive microglia in the Zucker lean control and Zucker diabetic fatty group were diffusely detected in the hippocampal CA1 region and dentate gyrus. There were no significant differences in the glial fibrillary acidic protein and Iba-1 immunoreactivity in the CA1 region and dentate gyrus between Zucker lean control and Zucker diabetic fatty groups. However, in the methimazole-treated Zucker lean control and Zucker diabetic fatty groups, the processes of glial fibrillary acidic protein tive astrocytes and Iba-1 immunoreactive microglia, were significantly decreased in both the CA1 region and dentate gyrus compared to that in the vehicle-treated Zucker lean control and Zucker diabetic fatty groups. These results suggest that diabetes has no effect on the morphology of astrocytes and microglia and that hypothyroidism during the onset of diabetes prominently reduces the processes of astrocytes and microglia.
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spelling pubmed-41461142014-09-09 Hypothyroidism affects astrocyte and microglial morphology in type 2 diabetes Nam, Sung Min Kim, Yo Na Yoo, Dae Young Yi, Sun Shin Choi, Jung Hoon Hwang, In Koo Seong, Je Kyung Yoon, Yeo Sung Neural Regen Res Technique and Method Article: Basic Research in Neural Regeneration In the present study, we investigated the effects of hypothyroidism on the morphology of astrocytes and microglia in the hippocampus of Zucker diabetic fatty rats and Zucker lean control rats. To induce hypothyroidism, Zucker lean control and Zucker diabetic fatty rats at 7 weeks of age orally received the vehicle or methimazole, an anti-thyroid drug, treatment for 5 weeks and were sacrificed at 12 weeks of age in all groups for blood chemistry and immunohistochemical staining. In the methimazole-treated Zucker lean control and Zucker diabetic fatty rats, the serum circulating thyronine (T(3)) and thyroxine (T(4)) levels were significantly decreased compared to levels observed in the vehicle-treated Zucker lean control or Zucker diabetic fatty rats. This reduction was more prominent in the methimazole-treated Zucker diabetic fatty group. Glial fibrillary acidic protein immunoreactive astrocytes and ionized calcium-binding adapter molecule 1 (Iba-1)-immunoreactive microglia in the Zucker lean control and Zucker diabetic fatty group were diffusely detected in the hippocampal CA1 region and dentate gyrus. There were no significant differences in the glial fibrillary acidic protein and Iba-1 immunoreactivity in the CA1 region and dentate gyrus between Zucker lean control and Zucker diabetic fatty groups. However, in the methimazole-treated Zucker lean control and Zucker diabetic fatty groups, the processes of glial fibrillary acidic protein tive astrocytes and Iba-1 immunoreactive microglia, were significantly decreased in both the CA1 region and dentate gyrus compared to that in the vehicle-treated Zucker lean control and Zucker diabetic fatty groups. These results suggest that diabetes has no effect on the morphology of astrocytes and microglia and that hypothyroidism during the onset of diabetes prominently reduces the processes of astrocytes and microglia. Medknow Publications & Media Pvt Ltd 2013-09-15 /pmc/articles/PMC4146114/ /pubmed/25206556 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.26.007 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technique and Method Article: Basic Research in Neural Regeneration
Nam, Sung Min
Kim, Yo Na
Yoo, Dae Young
Yi, Sun Shin
Choi, Jung Hoon
Hwang, In Koo
Seong, Je Kyung
Yoon, Yeo Sung
Hypothyroidism affects astrocyte and microglial morphology in type 2 diabetes
title Hypothyroidism affects astrocyte and microglial morphology in type 2 diabetes
title_full Hypothyroidism affects astrocyte and microglial morphology in type 2 diabetes
title_fullStr Hypothyroidism affects astrocyte and microglial morphology in type 2 diabetes
title_full_unstemmed Hypothyroidism affects astrocyte and microglial morphology in type 2 diabetes
title_short Hypothyroidism affects astrocyte and microglial morphology in type 2 diabetes
title_sort hypothyroidism affects astrocyte and microglial morphology in type 2 diabetes
topic Technique and Method Article: Basic Research in Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146114/
https://www.ncbi.nlm.nih.gov/pubmed/25206556
http://dx.doi.org/10.3969/j.issn.1673-5374.2013.26.007
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