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Noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells
The difference between Noggin and basic fibroblast growth factor for the neural precursor differentiation from human embryonic stem cells has not been studied. In this study, 100 μg/L Noggin or 20 μg/L basic fibroblast growth factor in serum-free neural induction medium was used to differentiate hum...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2013
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146116/ https://www.ncbi.nlm.nih.gov/pubmed/25206526 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.23.007 |
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author | Zhang, Yan Zhou, Junmei Fang, Zhenfu Jiang, Manxi Chen, Xuejin |
author_facet | Zhang, Yan Zhou, Junmei Fang, Zhenfu Jiang, Manxi Chen, Xuejin |
author_sort | Zhang, Yan |
collection | PubMed |
description | The difference between Noggin and basic fibroblast growth factor for the neural precursor differentiation from human embryonic stem cells has not been studied. In this study, 100 μg/L Noggin or 20 μg/L basic fibroblast growth factor in serum-free neural induction medium was used to differentiate human embryonic stem cells H14 into neural precursors using monolayer differentiation. Two weeks after induction, significantly higher numbers of neural rosettes formed in the Noggin-induced group than the basic fibroblast growth factor-induced group, as detected by phase contrast microscope. Immunofluorescence staining revealed expression levels of Nestin, β-III Tubulin and Sox-1 were higher in the induced cells and reverse-transcription PCR showed induced cells expressed Nestin, Sox-1 and Neurofilament mRNA. Protein and mRNA expression in the Noggin-induced group was increased compared with the basic fibroblast growth factor-induced group. Noggin has a greater effect than basic fibroblast growth factor on the induction of human embryonic stem cell differentiation into neural precursors by monolayer differentiation, as Noggin accelerates and increases the differentiation of neural precursors. |
format | Online Article Text |
id | pubmed-4146116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-41461162014-09-09 Noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells Zhang, Yan Zhou, Junmei Fang, Zhenfu Jiang, Manxi Chen, Xuejin Neural Regen Res Research and Report Article: Basic Research in Neural Regeneration The difference between Noggin and basic fibroblast growth factor for the neural precursor differentiation from human embryonic stem cells has not been studied. In this study, 100 μg/L Noggin or 20 μg/L basic fibroblast growth factor in serum-free neural induction medium was used to differentiate human embryonic stem cells H14 into neural precursors using monolayer differentiation. Two weeks after induction, significantly higher numbers of neural rosettes formed in the Noggin-induced group than the basic fibroblast growth factor-induced group, as detected by phase contrast microscope. Immunofluorescence staining revealed expression levels of Nestin, β-III Tubulin and Sox-1 were higher in the induced cells and reverse-transcription PCR showed induced cells expressed Nestin, Sox-1 and Neurofilament mRNA. Protein and mRNA expression in the Noggin-induced group was increased compared with the basic fibroblast growth factor-induced group. Noggin has a greater effect than basic fibroblast growth factor on the induction of human embryonic stem cell differentiation into neural precursors by monolayer differentiation, as Noggin accelerates and increases the differentiation of neural precursors. Medknow Publications & Media Pvt Ltd 2013-08-15 /pmc/articles/PMC4146116/ /pubmed/25206526 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.23.007 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research and Report Article: Basic Research in Neural Regeneration Zhang, Yan Zhou, Junmei Fang, Zhenfu Jiang, Manxi Chen, Xuejin Noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells |
title | Noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells |
title_full | Noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells |
title_fullStr | Noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells |
title_full_unstemmed | Noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells |
title_short | Noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells |
title_sort | noggin versus basic fibroblast growth factor on the differentiation of human embryonic stem cells |
topic | Research and Report Article: Basic Research in Neural Regeneration |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146116/ https://www.ncbi.nlm.nih.gov/pubmed/25206526 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.23.007 |
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