Which form of collagen is suitable for nerve cell culture?

In this study, we investigated the effects of hydrolyzed and non-hydrolyzed collagen and two-dimensional and three-dimensional collagen matrices on cell survival, attachment and neurite outgrowth of primary cultured nerve cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT)...

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Detalles Bibliográficos
Autores principales: Najafi, Mohsen Fathi, Zahri, Saber, Vahedi, Fatemeh, Toosi, Leila Esmaililian, Ariaee, Nazila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Research and Report Article: Basic Research in Neural Regeneration
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146121/
https://www.ncbi.nlm.nih.gov/pubmed/25206525
http://dx.doi.org/10.3969/j.issn.1673-5374.2013.23.006
Descripción
Sumario:In this study, we investigated the effects of hydrolyzed and non-hydrolyzed collagen and two-dimensional and three-dimensional collagen matrices on cell survival, attachment and neurite outgrowth of primary cultured nerve cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay and inverted microscopy. Hydrolyzed collagen facilitated nerve cell survival and neurite outgrowth, but it had no obvious influences on cell attachment. In contrast, non-hydrolyzed two-dimensional collagen matrix had no obvious effects on neurite outgrowth. These findings suggest that hydrolyzed collagen is an ideal nerve cell culture media.

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