Which form of collagen is suitable for nerve cell culture?

In this study, we investigated the effects of hydrolyzed and non-hydrolyzed collagen and two-dimensional and three-dimensional collagen matrices on cell survival, attachment and neurite outgrowth of primary cultured nerve cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT)...

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Autores principales: Najafi, Mohsen Fathi, Zahri, Saber, Vahedi, Fatemeh, Toosi, Leila Esmaililian, Ariaee, Nazila
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Research and Report Article: Basic Research in Neural Regeneration
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146121/
https://www.ncbi.nlm.nih.gov/pubmed/25206525
http://dx.doi.org/10.3969/j.issn.1673-5374.2013.23.006
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author Najafi, Mohsen Fathi
Zahri, Saber
Vahedi, Fatemeh
Toosi, Leila Esmaililian
Ariaee, Nazila
author_facet Najafi, Mohsen Fathi
Zahri, Saber
Vahedi, Fatemeh
Toosi, Leila Esmaililian
Ariaee, Nazila
author_sort Najafi, Mohsen Fathi
collection PubMed
description In this study, we investigated the effects of hydrolyzed and non-hydrolyzed collagen and two-dimensional and three-dimensional collagen matrices on cell survival, attachment and neurite outgrowth of primary cultured nerve cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay and inverted microscopy. Hydrolyzed collagen facilitated nerve cell survival and neurite outgrowth, but it had no obvious influences on cell attachment. In contrast, non-hydrolyzed two-dimensional collagen matrix had no obvious effects on neurite outgrowth. These findings suggest that hydrolyzed collagen is an ideal nerve cell culture media.
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spelling pubmed-41461212014-09-09 Which form of collagen is suitable for nerve cell culture? Najafi, Mohsen Fathi Zahri, Saber Vahedi, Fatemeh Toosi, Leila Esmaililian Ariaee, Nazila Neural Regen Res Research and Report Article: Basic Research in Neural Regeneration In this study, we investigated the effects of hydrolyzed and non-hydrolyzed collagen and two-dimensional and three-dimensional collagen matrices on cell survival, attachment and neurite outgrowth of primary cultured nerve cells using 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) colorimetric assay and inverted microscopy. Hydrolyzed collagen facilitated nerve cell survival and neurite outgrowth, but it had no obvious influences on cell attachment. In contrast, non-hydrolyzed two-dimensional collagen matrix had no obvious effects on neurite outgrowth. These findings suggest that hydrolyzed collagen is an ideal nerve cell culture media. Medknow Publications & Media Pvt Ltd 2013-08-15 /pmc/articles/PMC4146121/ /pubmed/25206525 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.23.006 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research and Report Article: Basic Research in Neural Regeneration
Najafi, Mohsen Fathi
Zahri, Saber
Vahedi, Fatemeh
Toosi, Leila Esmaililian
Ariaee, Nazila
Which form of collagen is suitable for nerve cell culture?
title Which form of collagen is suitable for nerve cell culture?
title_full Which form of collagen is suitable for nerve cell culture?
title_fullStr Which form of collagen is suitable for nerve cell culture?
title_full_unstemmed Which form of collagen is suitable for nerve cell culture?
title_short Which form of collagen is suitable for nerve cell culture?
title_sort which form of collagen is suitable for nerve cell culture?
topic Research and Report Article: Basic Research in Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146121/
https://www.ncbi.nlm.nih.gov/pubmed/25206525
http://dx.doi.org/10.3969/j.issn.1673-5374.2013.23.006
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