Oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-siRNA: Determination of an optimal transfection sequence

Toll-like receptor 3 protein expression has been shown to be upregulated during cerebral ischemia/reperfusion injury in rats. In this study, rat primary cortical neurons were subjected to oxygen-glucose deprivation to simulate cerebral ischemia/reperfusion injury. Chemically synthesized small interf...

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Autores principales: Cui, Guiyun, Wang, Xiaopeng, Ye, Xinchun, Zu, Jie, Zan, Kun, Hua, Fang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2013
Materias:
Technique and Method Article: Basic Research in Neural Regeneration
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146184/
https://www.ncbi.nlm.nih.gov/pubmed/25206644
http://dx.doi.org/10.3969/j.issn.1673-5374.2013.34.007
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author Cui, Guiyun
Wang, Xiaopeng
Ye, Xinchun
Zu, Jie
Zan, Kun
Hua, Fang
author_facet Cui, Guiyun
Wang, Xiaopeng
Ye, Xinchun
Zu, Jie
Zan, Kun
Hua, Fang
author_sort Cui, Guiyun
collection PubMed
description Toll-like receptor 3 protein expression has been shown to be upregulated during cerebral ischemia/reperfusion injury in rats. In this study, rat primary cortical neurons were subjected to oxygen-glucose deprivation to simulate cerebral ischemia/reperfusion injury. Chemically synthesized small interfering RNA (siRNA)-1280, -1724 and -418 specific to toll-like receptor 3 were transfected into oxygen-glucose deprived cortical neurons to suppress the upregulation of toll-like receptor 3 protein expression. Western blotting demonstrated that after transfection with siRNA, toll-like receptor 3 protein expression reduced, especially in the toll-like receptor 3-1724 group. These results suggested that siRNA-1724 is an optimal sequence for inhibiting toll-like receptor 3 expression in cortical neurons following oxygen-glucose deprivation.
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spelling pubmed-41461842014-09-09 Oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-siRNA: Determination of an optimal transfection sequence Cui, Guiyun Wang, Xiaopeng Ye, Xinchun Zu, Jie Zan, Kun Hua, Fang Neural Regen Res Technique and Method Article: Basic Research in Neural Regeneration Toll-like receptor 3 protein expression has been shown to be upregulated during cerebral ischemia/reperfusion injury in rats. In this study, rat primary cortical neurons were subjected to oxygen-glucose deprivation to simulate cerebral ischemia/reperfusion injury. Chemically synthesized small interfering RNA (siRNA)-1280, -1724 and -418 specific to toll-like receptor 3 were transfected into oxygen-glucose deprived cortical neurons to suppress the upregulation of toll-like receptor 3 protein expression. Western blotting demonstrated that after transfection with siRNA, toll-like receptor 3 protein expression reduced, especially in the toll-like receptor 3-1724 group. These results suggested that siRNA-1724 is an optimal sequence for inhibiting toll-like receptor 3 expression in cortical neurons following oxygen-glucose deprivation. Medknow Publications & Media Pvt Ltd 2013-12-05 /pmc/articles/PMC4146184/ /pubmed/25206644 http://dx.doi.org/10.3969/j.issn.1673-5374.2013.34.007 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technique and Method Article: Basic Research in Neural Regeneration
Cui, Guiyun
Wang, Xiaopeng
Ye, Xinchun
Zu, Jie
Zan, Kun
Hua, Fang
Oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-siRNA: Determination of an optimal transfection sequence
title Oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-siRNA: Determination of an optimal transfection sequence
title_full Oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-siRNA: Determination of an optimal transfection sequence
title_fullStr Oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-siRNA: Determination of an optimal transfection sequence
title_full_unstemmed Oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-siRNA: Determination of an optimal transfection sequence
title_short Oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-siRNA: Determination of an optimal transfection sequence
title_sort oxygen-glucose deprivation of neurons transfected with toll-like receptor 3-sirna: determination of an optimal transfection sequence
topic Technique and Method Article: Basic Research in Neural Regeneration
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146184/
https://www.ncbi.nlm.nih.gov/pubmed/25206644
http://dx.doi.org/10.3969/j.issn.1673-5374.2013.34.007
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