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Quercetin alleviates high glucose-induced Schwann cell damage by autophagy
Quercetin can reverse high glucose-induced inhibition of neural cell proliferation, and therefore may have a neuroprotective effect in diabetic peripheral neuropathy. It is difficult to obtain primary Schwann cells and RSC96 cells could replace primary Schwann cells in studies of the role of autopha...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2014
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146282/ https://www.ncbi.nlm.nih.gov/pubmed/25206782 http://dx.doi.org/10.4103/1673-5374.135328 |
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author | Qu, Ling Liang, Xiaochun Gu, Bei Liu, Wei |
author_facet | Qu, Ling Liang, Xiaochun Gu, Bei Liu, Wei |
author_sort | Qu, Ling |
collection | PubMed |
description | Quercetin can reverse high glucose-induced inhibition of neural cell proliferation, and therefore may have a neuroprotective effect in diabetic peripheral neuropathy. It is difficult to obtain primary Schwann cells and RSC96 cells could replace primary Schwann cells in studies of the role of autophagy in the mechanism underlying diabetic peripheral neuropathy. Here, we show that under high glucose conditions, there are fewer autophagosomes in immortalized rat RSC96 cells and primary rat Schwann cells than under control conditions, the proliferative activity of both cell types is significantly impaired, and the expression of Beclin-1 and LC3, the molecular markers for autophagy, is significantly lower. After intervention with quercetin, the autophagic and proliferative activity of both cell types is rescued. These results suggest that quercetin can alleviate high glucose-induced damage to Schwann cells by autophagy. |
format | Online Article Text |
id | pubmed-4146282 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-41462822014-09-09 Quercetin alleviates high glucose-induced Schwann cell damage by autophagy Qu, Ling Liang, Xiaochun Gu, Bei Liu, Wei Neural Regen Res Research and Report Quercetin can reverse high glucose-induced inhibition of neural cell proliferation, and therefore may have a neuroprotective effect in diabetic peripheral neuropathy. It is difficult to obtain primary Schwann cells and RSC96 cells could replace primary Schwann cells in studies of the role of autophagy in the mechanism underlying diabetic peripheral neuropathy. Here, we show that under high glucose conditions, there are fewer autophagosomes in immortalized rat RSC96 cells and primary rat Schwann cells than under control conditions, the proliferative activity of both cell types is significantly impaired, and the expression of Beclin-1 and LC3, the molecular markers for autophagy, is significantly lower. After intervention with quercetin, the autophagic and proliferative activity of both cell types is rescued. These results suggest that quercetin can alleviate high glucose-induced damage to Schwann cells by autophagy. Medknow Publications & Media Pvt Ltd 2014-06-15 /pmc/articles/PMC4146282/ /pubmed/25206782 http://dx.doi.org/10.4103/1673-5374.135328 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research and Report Qu, Ling Liang, Xiaochun Gu, Bei Liu, Wei Quercetin alleviates high glucose-induced Schwann cell damage by autophagy |
title | Quercetin alleviates high glucose-induced Schwann cell damage by autophagy |
title_full | Quercetin alleviates high glucose-induced Schwann cell damage by autophagy |
title_fullStr | Quercetin alleviates high glucose-induced Schwann cell damage by autophagy |
title_full_unstemmed | Quercetin alleviates high glucose-induced Schwann cell damage by autophagy |
title_short | Quercetin alleviates high glucose-induced Schwann cell damage by autophagy |
title_sort | quercetin alleviates high glucose-induced schwann cell damage by autophagy |
topic | Research and Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146282/ https://www.ncbi.nlm.nih.gov/pubmed/25206782 http://dx.doi.org/10.4103/1673-5374.135328 |
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