A non-invasive, rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms

The apolipoprotein E gene ε4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer's disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was ext...

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Autores principales: Yi, Li, Wu, Ting, Luo, Wenyuan, Zhou, Wen, Wu, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2014
Materias:
Technical Updates
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146311/
https://www.ncbi.nlm.nih.gov/pubmed/25206745
http://dx.doi.org/10.4103/1673-5374.125332
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author Yi, Li
Wu, Ting
Luo, Wenyuan
Zhou, Wen
Wu, Jun
author_facet Yi, Li
Wu, Ting
Luo, Wenyuan
Zhou, Wen
Wu, Jun
author_sort Yi, Li
collection PubMed
description The apolipoprotein E gene ε4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer's disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was extracted using magnetic nanoparticles, and genotyping was performed by real-time PCR using TaqMan-BHQ probes. Genotyping accuracy was validated by DNA sequencing. Our results demonstrate 100% correlation to DNA sequencing, indicating reliability of our protocol. Thus, the method we have developed for apolipoprotein E genotyping is accurate and reliable, and also suitable for genotyping large samples, which may help determine the role of the apolipoprotein E ε4 allele in neural regeneration in late-onset Alzheimer's disease cases.
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spelling pubmed-41463112014-09-09 A non-invasive, rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms Yi, Li Wu, Ting Luo, Wenyuan Zhou, Wen Wu, Jun Neural Regen Res Technical Updates The apolipoprotein E gene ε4 allele is considered a negative factor for neural regeneration in late-onset Alzheimer's disease cases. The aim of this study was to establish a non-invasive, rapid method to genotype apolipoprotein E gene polymorphisms. Genomic DNA from mouth swab specimens was extracted using magnetic nanoparticles, and genotyping was performed by real-time PCR using TaqMan-BHQ probes. Genotyping accuracy was validated by DNA sequencing. Our results demonstrate 100% correlation to DNA sequencing, indicating reliability of our protocol. Thus, the method we have developed for apolipoprotein E genotyping is accurate and reliable, and also suitable for genotyping large samples, which may help determine the role of the apolipoprotein E ε4 allele in neural regeneration in late-onset Alzheimer's disease cases. Medknow Publications & Media Pvt Ltd 2014-01-01 /pmc/articles/PMC4146311/ /pubmed/25206745 http://dx.doi.org/10.4103/1673-5374.125332 Text en Copyright: © Neural Regeneration Research http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Updates
Yi, Li
Wu, Ting
Luo, Wenyuan
Zhou, Wen
Wu, Jun
A non-invasive, rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms
title A non-invasive, rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms
title_full A non-invasive, rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms
title_fullStr A non-invasive, rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms
title_full_unstemmed A non-invasive, rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms
title_short A non-invasive, rapid method to genotype late-onset Alzheimer's disease-related apolipoprotein E gene polymorphisms
title_sort non-invasive, rapid method to genotype late-onset alzheimer's disease-related apolipoprotein e gene polymorphisms
topic Technical Updates
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146311/
https://www.ncbi.nlm.nih.gov/pubmed/25206745
http://dx.doi.org/10.4103/1673-5374.125332
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