Pantoea sp. Isolated from Tropical Fresh Water Exhibiting N-Acyl Homoserine Lactone Production

N-Acyl homoserine lactone (AHL) serves as signaling molecule for quorum sensing (QS) in Gram-negative bacteria to regulate various physiological activities including pathogenicity. With the aim of isolating freshwater-borne bacteria that can cause outbreak of disease in plants and portrayed QS prope...

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Autores principales: Tan, Wen-Si, Muhamad Yunos, Nina Yusrina, Tan, Pui-Wan, Mohamad, Nur Izzati, Adrian, Tan-Guan-Sheng, Yin, Wai-Fong, Chan, Kok-Gan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146356/
https://www.ncbi.nlm.nih.gov/pubmed/25197715
http://dx.doi.org/10.1155/2014/828971
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author Tan, Wen-Si
Muhamad Yunos, Nina Yusrina
Tan, Pui-Wan
Mohamad, Nur Izzati
Adrian, Tan-Guan-Sheng
Yin, Wai-Fong
Chan, Kok-Gan
author_facet Tan, Wen-Si
Muhamad Yunos, Nina Yusrina
Tan, Pui-Wan
Mohamad, Nur Izzati
Adrian, Tan-Guan-Sheng
Yin, Wai-Fong
Chan, Kok-Gan
author_sort Tan, Wen-Si
collection PubMed
description N-Acyl homoserine lactone (AHL) serves as signaling molecule for quorum sensing (QS) in Gram-negative bacteria to regulate various physiological activities including pathogenicity. With the aim of isolating freshwater-borne bacteria that can cause outbreak of disease in plants and portrayed QS properties, environmental water sampling was conducted. Here we report the preliminary screening of AHL production using Chromobacterium violaceum CV026 and Escherichia coli [pSB401] as AHL biosensors. The 16S rDNA gene sequence of isolate M009 showed the highest sequence similarity to Pantoea stewartii S9-116, which is a plant pathogen. The isolated Pantoea sp. was confirmed to produce N-3-oxohexanoyl-L-HSL (3-oxo-C6-HSL) through analysis of high resolution mass tandem mass spectrometry.
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spelling pubmed-41463562014-09-07 Pantoea sp. Isolated from Tropical Fresh Water Exhibiting N-Acyl Homoserine Lactone Production Tan, Wen-Si Muhamad Yunos, Nina Yusrina Tan, Pui-Wan Mohamad, Nur Izzati Adrian, Tan-Guan-Sheng Yin, Wai-Fong Chan, Kok-Gan ScientificWorldJournal Research Article N-Acyl homoserine lactone (AHL) serves as signaling molecule for quorum sensing (QS) in Gram-negative bacteria to regulate various physiological activities including pathogenicity. With the aim of isolating freshwater-borne bacteria that can cause outbreak of disease in plants and portrayed QS properties, environmental water sampling was conducted. Here we report the preliminary screening of AHL production using Chromobacterium violaceum CV026 and Escherichia coli [pSB401] as AHL biosensors. The 16S rDNA gene sequence of isolate M009 showed the highest sequence similarity to Pantoea stewartii S9-116, which is a plant pathogen. The isolated Pantoea sp. was confirmed to produce N-3-oxohexanoyl-L-HSL (3-oxo-C6-HSL) through analysis of high resolution mass tandem mass spectrometry. Hindawi Publishing Corporation 2014 2014-08-12 /pmc/articles/PMC4146356/ /pubmed/25197715 http://dx.doi.org/10.1155/2014/828971 Text en Copyright © 2014 Wen-Si Tan et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tan, Wen-Si
Muhamad Yunos, Nina Yusrina
Tan, Pui-Wan
Mohamad, Nur Izzati
Adrian, Tan-Guan-Sheng
Yin, Wai-Fong
Chan, Kok-Gan
Pantoea sp. Isolated from Tropical Fresh Water Exhibiting N-Acyl Homoserine Lactone Production
title Pantoea sp. Isolated from Tropical Fresh Water Exhibiting N-Acyl Homoserine Lactone Production
title_full Pantoea sp. Isolated from Tropical Fresh Water Exhibiting N-Acyl Homoserine Lactone Production
title_fullStr Pantoea sp. Isolated from Tropical Fresh Water Exhibiting N-Acyl Homoserine Lactone Production
title_full_unstemmed Pantoea sp. Isolated from Tropical Fresh Water Exhibiting N-Acyl Homoserine Lactone Production
title_short Pantoea sp. Isolated from Tropical Fresh Water Exhibiting N-Acyl Homoserine Lactone Production
title_sort pantoea sp. isolated from tropical fresh water exhibiting n-acyl homoserine lactone production
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146356/
https://www.ncbi.nlm.nih.gov/pubmed/25197715
http://dx.doi.org/10.1155/2014/828971
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