Cargando…

Yeast Killer Toxin-Like Candidacidal Ab6 Antibodies Elicited through the Manipulation of the Idiotypic Cascade

A mouse anti-anti-anti-idiotypic (Id) IgM monoclonal antibody (mAb K20, Ab4), functionally mimicking a Wyckerhamomyces anomalus (Pichia anomala) killer toxin (KT) characterized by fungicidal activity against yeasts presenting specific cell wall receptors (KTR) mainly constituted by β-1,3-glucan, was...

Descripción completa

Detalles Bibliográficos
Autores principales: Polonelli, Luciano, Beninati, Concetta, Teti, Giuseppe, Felici, Franco, Ciociola, Tecla, Giovati, Laura, Sperindè, Martina, Passo, Carla Lo, Pernice, Ida, Domina, Maria, Arigò, Milena, Papasergi, Salvatore, Mancuso, Giuseppe, Conti, Stefania, Magliani, Walter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146504/
https://www.ncbi.nlm.nih.gov/pubmed/25162681
http://dx.doi.org/10.1371/journal.pone.0105727
_version_ 1782332352166363136
author Polonelli, Luciano
Beninati, Concetta
Teti, Giuseppe
Felici, Franco
Ciociola, Tecla
Giovati, Laura
Sperindè, Martina
Passo, Carla Lo
Pernice, Ida
Domina, Maria
Arigò, Milena
Papasergi, Salvatore
Mancuso, Giuseppe
Conti, Stefania
Magliani, Walter
author_facet Polonelli, Luciano
Beninati, Concetta
Teti, Giuseppe
Felici, Franco
Ciociola, Tecla
Giovati, Laura
Sperindè, Martina
Passo, Carla Lo
Pernice, Ida
Domina, Maria
Arigò, Milena
Papasergi, Salvatore
Mancuso, Giuseppe
Conti, Stefania
Magliani, Walter
author_sort Polonelli, Luciano
collection PubMed
description A mouse anti-anti-anti-idiotypic (Id) IgM monoclonal antibody (mAb K20, Ab4), functionally mimicking a Wyckerhamomyces anomalus (Pichia anomala) killer toxin (KT) characterized by fungicidal activity against yeasts presenting specific cell wall receptors (KTR) mainly constituted by β-1,3-glucan, was produced from animals presenting anti-KT Abs (Ab3) following immunization with a rat IgM anti-Id KT-like mAb (mAb K10, Ab2). MAb K10 was produced by immunization with a KT-neutralizing mAb (mAb KT4, Ab1) bearing the internal image of KTR. MAb K20, likewise mAb K10, proved to be fungicidal in vitro against KT-sensitive Candida albicans cells, an activity neutralized by mAb KT4, and was capable of binding to β-1,3-glucan. MAb K20 and mAb K10 competed with each other and with KT for binding to C. albicans KTR. MAb K20 was used to identify peptide mimics of KTR by the selection of phage clones from random peptide phage display libraries. Using this strategy, four peptides (TK 1-4) were selected and used as immunogen in mice in the form of either keyhole limpet hemocyanin (KLH) conjugates or peptide-encoding minigenes. Peptide and DNA immunization could induce serum Abs characterized by candidacidal activity, which was inhibited by laminarin, a soluble β-1,3-glucan, but not by pustulan, a β-1,6-glucan. These findings show that the idiotypic cascade can not only overcome the barrier of animal species but also the nature of immunogens and the type of technology adopted.
format Online
Article
Text
id pubmed-4146504
institution National Center for Biotechnology Information
language English
publishDate 2014
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-41465042014-08-29 Yeast Killer Toxin-Like Candidacidal Ab6 Antibodies Elicited through the Manipulation of the Idiotypic Cascade Polonelli, Luciano Beninati, Concetta Teti, Giuseppe Felici, Franco Ciociola, Tecla Giovati, Laura Sperindè, Martina Passo, Carla Lo Pernice, Ida Domina, Maria Arigò, Milena Papasergi, Salvatore Mancuso, Giuseppe Conti, Stefania Magliani, Walter PLoS One Research Article A mouse anti-anti-anti-idiotypic (Id) IgM monoclonal antibody (mAb K20, Ab4), functionally mimicking a Wyckerhamomyces anomalus (Pichia anomala) killer toxin (KT) characterized by fungicidal activity against yeasts presenting specific cell wall receptors (KTR) mainly constituted by β-1,3-glucan, was produced from animals presenting anti-KT Abs (Ab3) following immunization with a rat IgM anti-Id KT-like mAb (mAb K10, Ab2). MAb K10 was produced by immunization with a KT-neutralizing mAb (mAb KT4, Ab1) bearing the internal image of KTR. MAb K20, likewise mAb K10, proved to be fungicidal in vitro against KT-sensitive Candida albicans cells, an activity neutralized by mAb KT4, and was capable of binding to β-1,3-glucan. MAb K20 and mAb K10 competed with each other and with KT for binding to C. albicans KTR. MAb K20 was used to identify peptide mimics of KTR by the selection of phage clones from random peptide phage display libraries. Using this strategy, four peptides (TK 1-4) were selected and used as immunogen in mice in the form of either keyhole limpet hemocyanin (KLH) conjugates or peptide-encoding minigenes. Peptide and DNA immunization could induce serum Abs characterized by candidacidal activity, which was inhibited by laminarin, a soluble β-1,3-glucan, but not by pustulan, a β-1,6-glucan. These findings show that the idiotypic cascade can not only overcome the barrier of animal species but also the nature of immunogens and the type of technology adopted. Public Library of Science 2014-08-27 /pmc/articles/PMC4146504/ /pubmed/25162681 http://dx.doi.org/10.1371/journal.pone.0105727 Text en © 2014 Polonelli et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Polonelli, Luciano
Beninati, Concetta
Teti, Giuseppe
Felici, Franco
Ciociola, Tecla
Giovati, Laura
Sperindè, Martina
Passo, Carla Lo
Pernice, Ida
Domina, Maria
Arigò, Milena
Papasergi, Salvatore
Mancuso, Giuseppe
Conti, Stefania
Magliani, Walter
Yeast Killer Toxin-Like Candidacidal Ab6 Antibodies Elicited through the Manipulation of the Idiotypic Cascade
title Yeast Killer Toxin-Like Candidacidal Ab6 Antibodies Elicited through the Manipulation of the Idiotypic Cascade
title_full Yeast Killer Toxin-Like Candidacidal Ab6 Antibodies Elicited through the Manipulation of the Idiotypic Cascade
title_fullStr Yeast Killer Toxin-Like Candidacidal Ab6 Antibodies Elicited through the Manipulation of the Idiotypic Cascade
title_full_unstemmed Yeast Killer Toxin-Like Candidacidal Ab6 Antibodies Elicited through the Manipulation of the Idiotypic Cascade
title_short Yeast Killer Toxin-Like Candidacidal Ab6 Antibodies Elicited through the Manipulation of the Idiotypic Cascade
title_sort yeast killer toxin-like candidacidal ab6 antibodies elicited through the manipulation of the idiotypic cascade
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4146504/
https://www.ncbi.nlm.nih.gov/pubmed/25162681
http://dx.doi.org/10.1371/journal.pone.0105727
work_keys_str_mv AT polonelliluciano yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT beninaticoncetta yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT tetigiuseppe yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT felicifranco yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT ciociolatecla yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT giovatilaura yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT sperindemartina yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT passocarlalo yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT perniceida yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT dominamaria yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT arigomilena yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT papasergisalvatore yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT mancusogiuseppe yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT contistefania yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade
AT maglianiwalter yeastkillertoxinlikecandidacidalab6antibodieselicitedthroughthemanipulationoftheidiotypiccascade