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The Role of Intrinsic Pathway in Apoptosis Activation and Progression in Peyronie's Disease

Peyronie's disease (PD) is characterized with formation of fibrous plaques which result in penile deformity, pain, and erectile dysfunction. The aim of this study was to investigate the activation of the intrinsic apoptotic pathway in plaques from PD patients. Tunica albuginea from either PD or...

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Autores principales: Loreto, Carla, La Rocca, Giampiero, Anzalone, Rita, Caltabiano, Rosario, Vespasiani, Giuseppe, Castorina, Sergio, Ralph, David J., Cellek, Selim, Musumeci, Giuseppe, Giunta, Salvatore, Djinovic, Rados, Basic, Dragoslav, Sansalone, Salvatore
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147380/
https://www.ncbi.nlm.nih.gov/pubmed/25197653
http://dx.doi.org/10.1155/2014/616149
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author Loreto, Carla
La Rocca, Giampiero
Anzalone, Rita
Caltabiano, Rosario
Vespasiani, Giuseppe
Castorina, Sergio
Ralph, David J.
Cellek, Selim
Musumeci, Giuseppe
Giunta, Salvatore
Djinovic, Rados
Basic, Dragoslav
Sansalone, Salvatore
author_facet Loreto, Carla
La Rocca, Giampiero
Anzalone, Rita
Caltabiano, Rosario
Vespasiani, Giuseppe
Castorina, Sergio
Ralph, David J.
Cellek, Selim
Musumeci, Giuseppe
Giunta, Salvatore
Djinovic, Rados
Basic, Dragoslav
Sansalone, Salvatore
author_sort Loreto, Carla
collection PubMed
description Peyronie's disease (PD) is characterized with formation of fibrous plaques which result in penile deformity, pain, and erectile dysfunction. The aim of this study was to investigate the activation of the intrinsic apoptotic pathway in plaques from PD patients. Tunica albuginea from either PD or control patients was assessed for the expression of bax, bcl-2 and caspases 9 and 3 using immunohistochemistry and by measurement of apoptotic cells using TUNEL assay. Bax overexpression was observed in metaplastic bone tissue, in fibroblasts, and in myofibroblast of plaques from PD patients. Little or no bcl-2 immunostaining was detected in samples from either patients or controls. Caspase 3 immunostaining was very strong in fibrous tissue, in metaplasic bone osteocytes, and in primary ossification center osteoblasts. Moderate caspase 9 immunostaining was seen in fibrous cells plaques and in osteocytes and osteoblasts of primary ossification centers from PD patients. Control samples were negative for caspase 9 immunostaining. In PD patients the TUNEL immunoassay showed intense immunostaining of fibroblasts and myofibroblasts, the absence of apoptotic cells in metaplasic bone tissue and on the border between fibrous and metaplastic bone tissue. Apoptosis occurs in stabilized PD plaques and is partly induced by the intrinsic pathway.
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spelling pubmed-41473802014-09-07 The Role of Intrinsic Pathway in Apoptosis Activation and Progression in Peyronie's Disease Loreto, Carla La Rocca, Giampiero Anzalone, Rita Caltabiano, Rosario Vespasiani, Giuseppe Castorina, Sergio Ralph, David J. Cellek, Selim Musumeci, Giuseppe Giunta, Salvatore Djinovic, Rados Basic, Dragoslav Sansalone, Salvatore Biomed Res Int Research Article Peyronie's disease (PD) is characterized with formation of fibrous plaques which result in penile deformity, pain, and erectile dysfunction. The aim of this study was to investigate the activation of the intrinsic apoptotic pathway in plaques from PD patients. Tunica albuginea from either PD or control patients was assessed for the expression of bax, bcl-2 and caspases 9 and 3 using immunohistochemistry and by measurement of apoptotic cells using TUNEL assay. Bax overexpression was observed in metaplastic bone tissue, in fibroblasts, and in myofibroblast of plaques from PD patients. Little or no bcl-2 immunostaining was detected in samples from either patients or controls. Caspase 3 immunostaining was very strong in fibrous tissue, in metaplasic bone osteocytes, and in primary ossification center osteoblasts. Moderate caspase 9 immunostaining was seen in fibrous cells plaques and in osteocytes and osteoblasts of primary ossification centers from PD patients. Control samples were negative for caspase 9 immunostaining. In PD patients the TUNEL immunoassay showed intense immunostaining of fibroblasts and myofibroblasts, the absence of apoptotic cells in metaplasic bone tissue and on the border between fibrous and metaplastic bone tissue. Apoptosis occurs in stabilized PD plaques and is partly induced by the intrinsic pathway. Hindawi Publishing Corporation 2014 2014-08-13 /pmc/articles/PMC4147380/ /pubmed/25197653 http://dx.doi.org/10.1155/2014/616149 Text en Copyright © 2014 Carla Loreto et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Loreto, Carla
La Rocca, Giampiero
Anzalone, Rita
Caltabiano, Rosario
Vespasiani, Giuseppe
Castorina, Sergio
Ralph, David J.
Cellek, Selim
Musumeci, Giuseppe
Giunta, Salvatore
Djinovic, Rados
Basic, Dragoslav
Sansalone, Salvatore
The Role of Intrinsic Pathway in Apoptosis Activation and Progression in Peyronie's Disease
title The Role of Intrinsic Pathway in Apoptosis Activation and Progression in Peyronie's Disease
title_full The Role of Intrinsic Pathway in Apoptosis Activation and Progression in Peyronie's Disease
title_fullStr The Role of Intrinsic Pathway in Apoptosis Activation and Progression in Peyronie's Disease
title_full_unstemmed The Role of Intrinsic Pathway in Apoptosis Activation and Progression in Peyronie's Disease
title_short The Role of Intrinsic Pathway in Apoptosis Activation and Progression in Peyronie's Disease
title_sort role of intrinsic pathway in apoptosis activation and progression in peyronie's disease
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147380/
https://www.ncbi.nlm.nih.gov/pubmed/25197653
http://dx.doi.org/10.1155/2014/616149
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