PA02.04. A comparative study of loha rasayana prepared by ayaskriti and modified method w.s.r. disintegration of Iron.

PURPOSE: Loha Rasayana one of the important Rasayana referred in Charaka Samhita is known for its Rasayana effect. This approach to the formulations was so rational and perfect that it fulfils the expectation of the scientific masses even also in terms of today's Nanoparticle theory. This technique got improvised with time and experience and is now used to make the Bhasmas. Purpose of present study was to scrutinize the validation of the process referred in Charaka Samhita to render the metal therapeutically suitable to the body and procedure by which the Bhasmas are prepared and to analyse them on ancient and modern parameters. METHOD: •Loha Rasayana was prepared as per Cha.Chi. 13/1523 by using Ayaskriti. •Samples were collected and subjected to analysis at different interval of time to trace the ongoing changes in the medicine. •Loha Rasayana was analytically compared with the Bhasma and also with a sample of Loha Rasayana prepared by using Bhasma on ancient and modern parameters. •The samples were subjected to XRD, ICPAES, SEM, Organoleptic tests, Physico chemical, Assay of Iron Content, Particle size, TLC and Alcohol Content. RESULT: Analytical Tests: LRA LRB Particle Size(200) 68.0% 88%, Particle Size (325) 4% 7%, TLC (Rf) 0.25 0.024, Alcohol content %2.12% 4.28%, ICP AES (Fe) % 66.45% 51.32%, Total Iron Content %36.40 43.50, Ferrous Content %7.23 7.09, Ferric Content % 29.49 37.91, XRD Hematite & Magnetite Hematite CONCLUSION: Submersion of Iron in Acidic media for 12 months reduced Iron to 68% and 88% of the Ayaskriti and Bhasma to pass through 200 mess size. Alcohol content of the samples was well within the limit (2.12% and 4.28) and Ferrous content were found to increase in both samples to 7.23% & 7.09%.