PA02.18. Phytochemical screening and antioxidant activity of Amrutha Choornam

PURPOSE: Background of the study: Diabetes mellitus is a common metabolic disorder characterized by high levels of blood glucose. Many studies have proven that oxidative stress plays an important role in Diabetes Mellitus. The etiology of oxidative stress in diabetes arises from excessive oxygen radical production from auto oxidation of glucose and glycosylation of protein, glycation of antioxidative enzymes. Aim: The present study evaluates Amrutha Choornam for its phytochemical and antioxidant property METHOD: The Sample was collected from The Arya vaidya pharmacy (Coimbatore) Ltd, Factory, Kanjikode. The preliminary phytochemical screening was determined using the methods by Harborne and the antioxidant activity was estimated by DPPH (2’2–diphenyl–1–picryl hydrazine) radical scavenging activity. RESULT: The three extracts of Amrutha choornam namely hexane, ethyl acetate and methanol were subjected to preliminary phytochemical screening. On comparing the results, the phytoconstituents were found to be higher in methanolic extract. Carbohydrates, phenols, tannins, Flavanoids, terpenoids, anthraquinones, and saponins were identified in the methanolic extract. In hexane extract carbohydrate, tannins, terpenoids Anthraquinones were present and in ethyl acetate extract showed the presence of carbohydrate, tannins, and terpenoids. The radical scavenging activity was found to be higher in the Ethyl acetate extract (7.9μg/ ml). The methanol extract possessed a better activity with an effective concentration of (9.8μg/ml). The less activity was found in hexane extract (2800μg/ml). CONCLUSION: The protective and preventive action of Amrutha Choornam in Diabetes mellitus could be due to its pronounced antioxidant activity. The potent phytochemicals in the formulation may be associated with its antidiabetic property.