PA02.20. A comparative study on the total phenolic content and antioxidant property of two Ayurvedic formulations–Indukantham gritham and Indukantham kashayam

PURPOSE: Background–: Indukantham Gritham is a formulation referred in the text of Sahasrayogam in the context of Gritha yogam. This formulation is also used in the water extract mode for treatment purpose. Indukantham combination is a potent formulation which enhances immunity with special indication in respiratory disorders and recurring fevers. Antioxidants play a significant role in improving the immunity and thereby in combating these conditions. Phenolic compounds are the most common water soluble antioxidant compounds found in plants. Objective–: The objective of the present work is to provide a comparison on the phytoconstituents, total phenolic content percentage and antioxidant properties of. Indukantham gritham and Indukantham Kashayam. METHOD: The samples were collected from The Arya Vaidya Pharmacy (Coimbatore) Limited. Qualitative phytochemical screening was carried out using the methods by Harborne. The total phenolic content percentage of these two modes were determined using Folin–ciocalteu's method and the antioxidant activities were estimated by DPPH (2,2–DiPhenyl–1–Picryl Hydrazine) radical scavenging activity. RESULT: Indukantham gritham showed the presence of carbohydrates, phenols, terpenoids and steroids, whereas Indukantham Kashayam showed the presence of carbohydrates, phenols, saponins. Indukantham Kashayam exhibited higher phenolic content and antioxidant activity (% TPC–11.91%,IC50–0.10mg/ml) when compared to Indukantham gritham (0.03%, IC50–106mg/ml). CONCLUSION: The higher phenolic content of Kashayam may be due to the water soluble nature of phenols which in turn imparts higher antioxidant activity. The extraction of fat soluble components along with water soluble components may be a reason for the higher number of phytoconstituents in gritham. The comparative study explores the difference in phytoconstituents and antioxidant properties of the Indukantham combination when formulated in two different modes.