OA02.01. Fibroblast growth inhibitory and antiinflammatory effect of Curcumin extract: A cell culture study

PURPOSE: Curcumin is an active principle obtained from the plant curcuma longa which is not a new therapeutic tool but proved long time back with lots of medicinal values in traditional medicine. This dried rhizome powder extract had been studied extensively in animal models and proved with antiinflammatory activity. Many human studies had proved its antiinflammatory action and its effectiveness in reducing inflammation in osteoarthritis and its benefits in rheumatoid arthritis. Because this extract widely used for antiinflammatory action in arthritis and help in healing a wound we had planned to compare the effectiveness with known antiinflammatory agent methyl prednesolone. METHOD: Curcumin extract, fibroblast cells L6 rat skeletal muscle cell line (NCCS, Pune), Medium DMEM (Dulbecco's Modified Eagles Medium) with 10% fetal bovine serum in 5% CO2 at 37°C, Drug Prednisolone and Curcumin successive extract. Curcumin extract: Locally available Curcumin rhizomes was purchased after confirmation with botanical survey department of India, Coimbatore, allowed drying at shade for three to four months after cleaning with water to remove the mud and dust. After three months Rhizomes were powdered. This powdered cumin were used to prepare successive extract as per the standard technique using chloroform and ethanol with the help of Soxhlet apparatus for cell culture study with the varying doses of 100μg, 300μg, 600μg, 900μg and 1200μg using MIT Assay. RESULT: After 24hrs of the drug treatment, the medium was changed for all the groups and 10 μl of MTT (5 mg/ml stock solution) was added and the plates were incubated for an additional 4 hrs. The medium was discarded and the formazan blue, which was formed in the cells, was dissolved with 50 μl of DMSO. The optical density was measured at 570 nm. The percentage toxicity was calculated & IC 50 was calculated by using Grapad PRISM software tool following formula. CONCLUSION: Curcumin extract has role in chronic inflammation based on the inhibitory role on connective tissue cell fibroblast proliferation when compared with the known established anti-inflammatory agent methyl prednisolone.