Cargando…
Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients
Glioma is the most common and believed to be one of the most aggressive tumors of the central nervous system (CNS) in humans. Very little information is available on the etiology and pathogenesis of these tumors to date. A significant gap remains in our current understanding of the molecular pathway...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147641/ https://www.ncbi.nlm.nih.gov/pubmed/25170298 http://dx.doi.org/10.7150/ijms.9412 |
_version_ | 1782332485078614016 |
---|---|
author | Shamran, Haidar A. Hamza, Subah J. Yaseen, Nahi Y. Al-Juboory, Ahmad A. Taub, Dennis D Price, Robert L. Nagarkatti, Mitzi Nagarkatti, Prakash S. Singh, Udai P. |
author_facet | Shamran, Haidar A. Hamza, Subah J. Yaseen, Nahi Y. Al-Juboory, Ahmad A. Taub, Dennis D Price, Robert L. Nagarkatti, Mitzi Nagarkatti, Prakash S. Singh, Udai P. |
author_sort | Shamran, Haidar A. |
collection | PubMed |
description | Glioma is the most common and believed to be one of the most aggressive tumors of the central nervous system (CNS) in humans. Very little information is available on the etiology and pathogenesis of these tumors to date. A significant gap remains in our current understanding of the molecular pathways involved in the genesis, progression and clinical behavior of these tumors. Recently, several single nucleotide polymorphisms (SNPs) have been identified in cytokine gene sequences, particularly within the promoter region of these genes, and have been shown to be associated with the development of different types of brain tumors. The present study investigates the association of C-33T SNP in the interleukin-4 (IL-4) gene with systemic IL-4 level and the S503P SNP in the IL-4R gene with the incidence of glioma. Blood samples were collected from 100 histologically confirmed adult patients with glioma, and 30 apparently healthy individuals from the same area. DNA was extracted from each blood sample, and the IL-4 and IL-4R genes were amplified using polymerase chain reaction (PCR) with gene-specific primers. Systemic IL-4 concentration was assessed in serum samples from each participant by enzyme-linked immunosorbent assay (ELISA). We observed a negative association between the homozygous genotype (CC) of the SNP C-33T of the IL-4 gene with the incidence of glioma (OR=0.19, 95% CI=0.035-1.02), while the T allele of the SNP demonstrated a significant protective association against glioma. Similarly, the heterozygous (CT) and homozygous mutant (CC) of the SNP S503P of the IL-4R gene demonstrated a significant association with glioma development (OR=0.405, 95% CI=0.17-0.969 and OR=0.147, 95% CI=0.036-0.6 respectively), while the C allele exhibited a highly significant association with protection from glioma formation. These findings suggest that the T allele of the SNP C-33T in the IL-4 gene and the C allele of the SNP S503P in IL-4R may have a protective role against glioma development. |
format | Online Article Text |
id | pubmed-4147641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-41476412014-08-28 Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients Shamran, Haidar A. Hamza, Subah J. Yaseen, Nahi Y. Al-Juboory, Ahmad A. Taub, Dennis D Price, Robert L. Nagarkatti, Mitzi Nagarkatti, Prakash S. Singh, Udai P. Int J Med Sci Research Paper Glioma is the most common and believed to be one of the most aggressive tumors of the central nervous system (CNS) in humans. Very little information is available on the etiology and pathogenesis of these tumors to date. A significant gap remains in our current understanding of the molecular pathways involved in the genesis, progression and clinical behavior of these tumors. Recently, several single nucleotide polymorphisms (SNPs) have been identified in cytokine gene sequences, particularly within the promoter region of these genes, and have been shown to be associated with the development of different types of brain tumors. The present study investigates the association of C-33T SNP in the interleukin-4 (IL-4) gene with systemic IL-4 level and the S503P SNP in the IL-4R gene with the incidence of glioma. Blood samples were collected from 100 histologically confirmed adult patients with glioma, and 30 apparently healthy individuals from the same area. DNA was extracted from each blood sample, and the IL-4 and IL-4R genes were amplified using polymerase chain reaction (PCR) with gene-specific primers. Systemic IL-4 concentration was assessed in serum samples from each participant by enzyme-linked immunosorbent assay (ELISA). We observed a negative association between the homozygous genotype (CC) of the SNP C-33T of the IL-4 gene with the incidence of glioma (OR=0.19, 95% CI=0.035-1.02), while the T allele of the SNP demonstrated a significant protective association against glioma. Similarly, the heterozygous (CT) and homozygous mutant (CC) of the SNP S503P of the IL-4R gene demonstrated a significant association with glioma development (OR=0.405, 95% CI=0.17-0.969 and OR=0.147, 95% CI=0.036-0.6 respectively), while the C allele exhibited a highly significant association with protection from glioma formation. These findings suggest that the T allele of the SNP C-33T in the IL-4 gene and the C allele of the SNP S503P in IL-4R may have a protective role against glioma development. Ivyspring International Publisher 2014-08-22 /pmc/articles/PMC4147641/ /pubmed/25170298 http://dx.doi.org/10.7150/ijms.9412 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
spellingShingle | Research Paper Shamran, Haidar A. Hamza, Subah J. Yaseen, Nahi Y. Al-Juboory, Ahmad A. Taub, Dennis D Price, Robert L. Nagarkatti, Mitzi Nagarkatti, Prakash S. Singh, Udai P. Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients |
title | Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients |
title_full | Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients |
title_fullStr | Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients |
title_full_unstemmed | Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients |
title_short | Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients |
title_sort | impact of single nucleotide polymorphism in il-4, il-4r genes and systemic concentration of il-4 on the incidence of glioma in iraqi patients |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147641/ https://www.ncbi.nlm.nih.gov/pubmed/25170298 http://dx.doi.org/10.7150/ijms.9412 |
work_keys_str_mv | AT shamranhaidara impactofsinglenucleotidepolymorphisminil4il4rgenesandsystemicconcentrationofil4ontheincidenceofgliomainiraqipatients AT hamzasubahj impactofsinglenucleotidepolymorphisminil4il4rgenesandsystemicconcentrationofil4ontheincidenceofgliomainiraqipatients AT yaseennahiy impactofsinglenucleotidepolymorphisminil4il4rgenesandsystemicconcentrationofil4ontheincidenceofgliomainiraqipatients AT aljubooryahmada impactofsinglenucleotidepolymorphisminil4il4rgenesandsystemicconcentrationofil4ontheincidenceofgliomainiraqipatients AT taubdennisd impactofsinglenucleotidepolymorphisminil4il4rgenesandsystemicconcentrationofil4ontheincidenceofgliomainiraqipatients AT pricerobertl impactofsinglenucleotidepolymorphisminil4il4rgenesandsystemicconcentrationofil4ontheincidenceofgliomainiraqipatients AT nagarkattimitzi impactofsinglenucleotidepolymorphisminil4il4rgenesandsystemicconcentrationofil4ontheincidenceofgliomainiraqipatients AT nagarkattiprakashs impactofsinglenucleotidepolymorphisminil4il4rgenesandsystemicconcentrationofil4ontheincidenceofgliomainiraqipatients AT singhudaip impactofsinglenucleotidepolymorphisminil4il4rgenesandsystemicconcentrationofil4ontheincidenceofgliomainiraqipatients |