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Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients

Glioma is the most common and believed to be one of the most aggressive tumors of the central nervous system (CNS) in humans. Very little information is available on the etiology and pathogenesis of these tumors to date. A significant gap remains in our current understanding of the molecular pathway...

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Autores principales: Shamran, Haidar A., Hamza, Subah J., Yaseen, Nahi Y., Al-Juboory, Ahmad A., Taub, Dennis D, Price, Robert L., Nagarkatti, Mitzi, Nagarkatti, Prakash S., Singh, Udai P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147641/
https://www.ncbi.nlm.nih.gov/pubmed/25170298
http://dx.doi.org/10.7150/ijms.9412
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author Shamran, Haidar A.
Hamza, Subah J.
Yaseen, Nahi Y.
Al-Juboory, Ahmad A.
Taub, Dennis D
Price, Robert L.
Nagarkatti, Mitzi
Nagarkatti, Prakash S.
Singh, Udai P.
author_facet Shamran, Haidar A.
Hamza, Subah J.
Yaseen, Nahi Y.
Al-Juboory, Ahmad A.
Taub, Dennis D
Price, Robert L.
Nagarkatti, Mitzi
Nagarkatti, Prakash S.
Singh, Udai P.
author_sort Shamran, Haidar A.
collection PubMed
description Glioma is the most common and believed to be one of the most aggressive tumors of the central nervous system (CNS) in humans. Very little information is available on the etiology and pathogenesis of these tumors to date. A significant gap remains in our current understanding of the molecular pathways involved in the genesis, progression and clinical behavior of these tumors. Recently, several single nucleotide polymorphisms (SNPs) have been identified in cytokine gene sequences, particularly within the promoter region of these genes, and have been shown to be associated with the development of different types of brain tumors. The present study investigates the association of C-33T SNP in the interleukin-4 (IL-4) gene with systemic IL-4 level and the S503P SNP in the IL-4R gene with the incidence of glioma. Blood samples were collected from 100 histologically confirmed adult patients with glioma, and 30 apparently healthy individuals from the same area. DNA was extracted from each blood sample, and the IL-4 and IL-4R genes were amplified using polymerase chain reaction (PCR) with gene-specific primers. Systemic IL-4 concentration was assessed in serum samples from each participant by enzyme-linked immunosorbent assay (ELISA). We observed a negative association between the homozygous genotype (CC) of the SNP C-33T of the IL-4 gene with the incidence of glioma (OR=0.19, 95% CI=0.035-1.02), while the T allele of the SNP demonstrated a significant protective association against glioma. Similarly, the heterozygous (CT) and homozygous mutant (CC) of the SNP S503P of the IL-4R gene demonstrated a significant association with glioma development (OR=0.405, 95% CI=0.17-0.969 and OR=0.147, 95% CI=0.036-0.6 respectively), while the C allele exhibited a highly significant association with protection from glioma formation. These findings suggest that the T allele of the SNP C-33T in the IL-4 gene and the C allele of the SNP S503P in IL-4R may have a protective role against glioma development.
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spelling pubmed-41476412014-08-28 Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients Shamran, Haidar A. Hamza, Subah J. Yaseen, Nahi Y. Al-Juboory, Ahmad A. Taub, Dennis D Price, Robert L. Nagarkatti, Mitzi Nagarkatti, Prakash S. Singh, Udai P. Int J Med Sci Research Paper Glioma is the most common and believed to be one of the most aggressive tumors of the central nervous system (CNS) in humans. Very little information is available on the etiology and pathogenesis of these tumors to date. A significant gap remains in our current understanding of the molecular pathways involved in the genesis, progression and clinical behavior of these tumors. Recently, several single nucleotide polymorphisms (SNPs) have been identified in cytokine gene sequences, particularly within the promoter region of these genes, and have been shown to be associated with the development of different types of brain tumors. The present study investigates the association of C-33T SNP in the interleukin-4 (IL-4) gene with systemic IL-4 level and the S503P SNP in the IL-4R gene with the incidence of glioma. Blood samples were collected from 100 histologically confirmed adult patients with glioma, and 30 apparently healthy individuals from the same area. DNA was extracted from each blood sample, and the IL-4 and IL-4R genes were amplified using polymerase chain reaction (PCR) with gene-specific primers. Systemic IL-4 concentration was assessed in serum samples from each participant by enzyme-linked immunosorbent assay (ELISA). We observed a negative association between the homozygous genotype (CC) of the SNP C-33T of the IL-4 gene with the incidence of glioma (OR=0.19, 95% CI=0.035-1.02), while the T allele of the SNP demonstrated a significant protective association against glioma. Similarly, the heterozygous (CT) and homozygous mutant (CC) of the SNP S503P of the IL-4R gene demonstrated a significant association with glioma development (OR=0.405, 95% CI=0.17-0.969 and OR=0.147, 95% CI=0.036-0.6 respectively), while the C allele exhibited a highly significant association with protection from glioma formation. These findings suggest that the T allele of the SNP C-33T in the IL-4 gene and the C allele of the SNP S503P in IL-4R may have a protective role against glioma development. Ivyspring International Publisher 2014-08-22 /pmc/articles/PMC4147641/ /pubmed/25170298 http://dx.doi.org/10.7150/ijms.9412 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.
spellingShingle Research Paper
Shamran, Haidar A.
Hamza, Subah J.
Yaseen, Nahi Y.
Al-Juboory, Ahmad A.
Taub, Dennis D
Price, Robert L.
Nagarkatti, Mitzi
Nagarkatti, Prakash S.
Singh, Udai P.
Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients
title Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients
title_full Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients
title_fullStr Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients
title_full_unstemmed Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients
title_short Impact of Single Nucleotide Polymorphism in IL-4, IL-4R Genes and Systemic Concentration of IL-4 on the Incidence of Glioma in Iraqi Patients
title_sort impact of single nucleotide polymorphism in il-4, il-4r genes and systemic concentration of il-4 on the incidence of glioma in iraqi patients
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147641/
https://www.ncbi.nlm.nih.gov/pubmed/25170298
http://dx.doi.org/10.7150/ijms.9412
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