Cargando…
Heterogeneity of Osteosarcoma Cell Lines Led to Variable Responses in Reprogramming
Four osteosarcoma cell lines, Saos-2, MG-63, G-292 and U-2 OS, were reprogrammed to pluripotent state using Yamanaka factors retroviral transduction method. Embryonic stem cell (ESC)-like clusters started to appear between 15 to 20 days post transduction. Morphology of the colonies resembled that of...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2014
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147642/ https://www.ncbi.nlm.nih.gov/pubmed/25170299 http://dx.doi.org/10.7150/ijms.8281 |
_version_ | 1782332485310349312 |
---|---|
author | Choong, Pei Feng Teh, Hui Xin Teoh, Hoon Koon Ong, Han Kiat Choo, Kong Bung Sugii, Shigeki Cheong, Soon Keng Kamarul, Tunku |
author_facet | Choong, Pei Feng Teh, Hui Xin Teoh, Hoon Koon Ong, Han Kiat Choo, Kong Bung Sugii, Shigeki Cheong, Soon Keng Kamarul, Tunku |
author_sort | Choong, Pei Feng |
collection | PubMed |
description | Four osteosarcoma cell lines, Saos-2, MG-63, G-292 and U-2 OS, were reprogrammed to pluripotent state using Yamanaka factors retroviral transduction method. Embryonic stem cell (ESC)-like clusters started to appear between 15 to 20 days post transduction. Morphology of the colonies resembled that of ESC colonies with defined border and tightly-packed cells. The reprogrammed sarcomas expressed alkaline phosphatase and pluripotency markers, OCT4, SSEA4, TRA-1-60 and TRA-1-81, as in ESC up to Passage 15. All reprogrammed sarcomas could form embryoid body-like spheres when cultured in suspension in a low attachment dish for up to 10 days. Further testing on the directed differentiation capacity of the reprogrammed sarcomas showed all four reprogrammed sarcoma lines could differentiate into adipocytes while reprogrammed Saos-2-REP, MG-63-REP and G-292-REP could differentiate into osteocytes. Among the 4 osteosarcoma cell lines, U-2 OS reported the highest transduction efficiency but recorded the lowest reprogramming stability under long term culture. Thus, there may be intrinsic differences governing the variable responses of osteosarcoma cell lines towards reprogramming and long term culture effect of the reprogrammed cells. This is a first report to associate intrinsic factors in different osteosarcoma cell lines with variable reprogramming responses and effects on the reprogrammed cells after prolonged culture. |
format | Online Article Text |
id | pubmed-4147642 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2014 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-41476422014-08-28 Heterogeneity of Osteosarcoma Cell Lines Led to Variable Responses in Reprogramming Choong, Pei Feng Teh, Hui Xin Teoh, Hoon Koon Ong, Han Kiat Choo, Kong Bung Sugii, Shigeki Cheong, Soon Keng Kamarul, Tunku Int J Med Sci Short Research Communication Four osteosarcoma cell lines, Saos-2, MG-63, G-292 and U-2 OS, were reprogrammed to pluripotent state using Yamanaka factors retroviral transduction method. Embryonic stem cell (ESC)-like clusters started to appear between 15 to 20 days post transduction. Morphology of the colonies resembled that of ESC colonies with defined border and tightly-packed cells. The reprogrammed sarcomas expressed alkaline phosphatase and pluripotency markers, OCT4, SSEA4, TRA-1-60 and TRA-1-81, as in ESC up to Passage 15. All reprogrammed sarcomas could form embryoid body-like spheres when cultured in suspension in a low attachment dish for up to 10 days. Further testing on the directed differentiation capacity of the reprogrammed sarcomas showed all four reprogrammed sarcoma lines could differentiate into adipocytes while reprogrammed Saos-2-REP, MG-63-REP and G-292-REP could differentiate into osteocytes. Among the 4 osteosarcoma cell lines, U-2 OS reported the highest transduction efficiency but recorded the lowest reprogramming stability under long term culture. Thus, there may be intrinsic differences governing the variable responses of osteosarcoma cell lines towards reprogramming and long term culture effect of the reprogrammed cells. This is a first report to associate intrinsic factors in different osteosarcoma cell lines with variable reprogramming responses and effects on the reprogrammed cells after prolonged culture. Ivyspring International Publisher 2014-08-22 /pmc/articles/PMC4147642/ /pubmed/25170299 http://dx.doi.org/10.7150/ijms.8281 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
spellingShingle | Short Research Communication Choong, Pei Feng Teh, Hui Xin Teoh, Hoon Koon Ong, Han Kiat Choo, Kong Bung Sugii, Shigeki Cheong, Soon Keng Kamarul, Tunku Heterogeneity of Osteosarcoma Cell Lines Led to Variable Responses in Reprogramming |
title | Heterogeneity of Osteosarcoma Cell Lines Led to Variable Responses in Reprogramming |
title_full | Heterogeneity of Osteosarcoma Cell Lines Led to Variable Responses in Reprogramming |
title_fullStr | Heterogeneity of Osteosarcoma Cell Lines Led to Variable Responses in Reprogramming |
title_full_unstemmed | Heterogeneity of Osteosarcoma Cell Lines Led to Variable Responses in Reprogramming |
title_short | Heterogeneity of Osteosarcoma Cell Lines Led to Variable Responses in Reprogramming |
title_sort | heterogeneity of osteosarcoma cell lines led to variable responses in reprogramming |
topic | Short Research Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4147642/ https://www.ncbi.nlm.nih.gov/pubmed/25170299 http://dx.doi.org/10.7150/ijms.8281 |
work_keys_str_mv | AT choongpeifeng heterogeneityofosteosarcomacelllinesledtovariableresponsesinreprogramming AT tehhuixin heterogeneityofosteosarcomacelllinesledtovariableresponsesinreprogramming AT teohhoonkoon heterogeneityofosteosarcomacelllinesledtovariableresponsesinreprogramming AT onghankiat heterogeneityofosteosarcomacelllinesledtovariableresponsesinreprogramming AT chookongbung heterogeneityofosteosarcomacelllinesledtovariableresponsesinreprogramming AT sugiishigeki heterogeneityofosteosarcomacelllinesledtovariableresponsesinreprogramming AT cheongsoonkeng heterogeneityofosteosarcomacelllinesledtovariableresponsesinreprogramming AT kamarultunku heterogeneityofosteosarcomacelllinesledtovariableresponsesinreprogramming |